Effects of topical chondrocyte-derived extracellular matrix treatment on corneal wound healing, following an alkali burn injury

Abstract

Abstract. Numerous treatments have been used in the management of corneal chemical burns; however, no optimal treatment for corneal chemical burns currently exists. The present study investigated the effects of topical chondrocyte-derived extracellular matrix (CD-ECM) treatment on corneal wound healing, using an alkali burn mouse model. Topical treatment with CD-ECM was shown to reduce corneal opacity following an alkali burn. A histological examination observed the presence of regenerated epithelial cells and a small number of inflammatory cells in the corneas of CD-ECM-treated mice. The majority of the inflammatory cells present in the corneas of the phosphate-buffered saline (PBS)-treated mice were neutrophils that expressed matrix metalloproteinase (MMP)-9. The amount of neutrophils was significantly reduced in the corneas of the CD-ECM-treated mice. Furthermore, the expression levels of interleukin (IL)-8 were significantly reduced in the CD-ECM treatment group, but not in the mice that received the PBS treatment. The results of the present study indicate that CD-ECM treatment may accelerate wound healing in a model of alkali burn-induced corneal injury. The therapeutic mechanism may be associated with accelerated reepithelialization and reduced recruitment of MMP-9-expressing neutrophils, through inhibiting the production of IL-8.

Ophthalmic Combination of SurR9-C84A and Trichostatin-A Targeting Molecular Pathogenesis of Alkali Burn

“…Corneal insults induced by the alkali burn represent another complex therapeutic challenge for the ophthalmologist since the injury can progress to an severe corneal ulceration, perforation, opacification, and surface curvature alteration culminating in permanent visual impairment (Okada et al, 2014 ). Numerous studies have focused on the control and prevention of this disease progression with a thorough review of the treatment modalities against the alkali insults (Pattamatta et al, 2013 ; Ambrosone et al, 2014 ; Okada et al, 2014 ; Yang et al, 2015 ). Alkali burns are known to destroy not only the epithelium but also the stroma, marked by the presence of large vacuolated structures in the stroma (Zhang et al, 2005 ).…”

Anti-inflammatory effect of hydroxyproline-GQDGLAGPK in desiccation stress-induced experimental dry eye mouse

“…These components serves an important role in tissue and organ morphogenesis, and induce the surrounding cells to repair the wounded tissue instead of forming scar tissue 24 , 25 . The ECM also prevents the triggering of immune responses that are associated with inflammation 26 – 28 . Our previous reports have shown that chondrocyte-derived ECM (CDECM) suppressed corneal neovascularization (NV) and opacity by modulating the inflammatory reaction in alkaline burn animal models 28 , 29 .…”

“…The ECM also prevents the triggering of immune responses that are associated with inflammation 26 – 28 . Our previous reports have shown that chondrocyte-derived ECM (CDECM) suppressed corneal neovascularization (NV) and opacity by modulating the inflammatory reaction in alkaline burn animal models 28 , 29 . Furthermore, we reported that CDECM has anti-angiogenic effects in pterygium mouse and suture-induced corneal NV rabbits 30 , 31 .…”

Topical Ophthalmic Formulation of Trichostatin A and SurR9-C84A for Quick Recovery Post-alkali Burn of Corneal Haze

“…Several studies have focused on control and prevention of alkali burn as it’s a very common and has severe effects ( Pattamatta et al, 2013 ; Ambrosone et al, 2014 ; Okada et al, 2014 ; Yang et al, 2015 ). Alkali burns can destroy not only the corneal epithelium but also the corneal stroma, by creating large vacuolated structures ( Zhang et al, 2005 ).…”