Effects of topical treatment of foot rot in sheep using ozonated olive ointment

Abstract

Introduction Foot rot in small ruminants is highly contagious, causes severe lameness, and impairs fertility and wool and meat production. It is usually treated with parenteral antibiotics, with attendant antibiotic resistance risk, and with bactericidal footbaths, potentially harmful to humans and the environment. An alternative treatment in sheep is proposed based on repeated topical ozonated ointment application. Its effectiveness and safety were evaluated by estimation of acute-phase response, biochemical indicators of organic damage, and antioxidant/oxidant balance (AOB). Material and Methods The study was conducted on ten sheep with Egerton scale 2–3 lesions. Ozone application was repeated every day for seven days. Blood was drawn first (T0) after foot cleaning and before ozonation, then (T1) seven days after the first ozone application, and finally (T2) four days after the last application. Results High clinical effectiveness was observed, with total recovery by 28 days from the start of treatment. A significant increase in antiradical activity was noted on the basis of a 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assay from 1.16 ± 0.04 μmolTe/mL at T0 to 1.23 ± 0.03 μmolTe/mL at T1, with a slight decrease in oxidative stress. Calculated on the basis of antiradical capacity, AOB was higher at T1 (130 ± 19%) and decreased to 110 ± 16% at T2. Calculated on the basis of reducing power, it was 169 ± 22% at T1 and 131 ± 17% at T2. Conclusion These results indicated that the AOB is efficient enough to prevent oxidative organ injury and the applied doses of ozone are safe for animals.

Ozonioterapia Em Animais Domésticos: Conceitos Básicos E Diretrizes

“…Os estudos de ozonioterapia em animais incluem os efeitos da ozonioterapia local e sistêmica em cães (Han et al, 2007;Moda et al, 2022), bovinos (Zobel et al, 2014;Enginler et al;, equinos (Vendruscolo et al;2018, Ávila et al, 2022, ovinos (Duricic et al, 2016;Szponder et al, 2021) e caprinos Skliarov et al;.…”

A Comparative Study of the Chemical Properties and Antibacterial Activity of Four Different Ozonated Oils for Veterinary Purposes

“…Ozonated olive oil has been used effectively to treat cat ear mites (Otodectes cynotis) [25]. The ozonated oil ointment used in the study by Szponder T. et al proved to be an effective and convenient means for the topical treatment of foot rot in sheep enabling precise administration and causing no undesirable effects [26]. Ozonated oils in liposomes plus hypromellose have been reported to have restorative and regenerative effects in external spontaneous ocular pathologies in animals and humans [27].…”