Effects of toxic Microcystis aeruginosa on the silver carp Hypophthalmichtys molitrix revealed by hepatic RNA-seq and miRNA-seq

Hu, Menghong; Qu, Xiancheng; Pan, Lisha; Fu, Chunxue; Jia, Peixuan; Liu, Qigen; Wang, Youji

doi:10.1038/s41598-017-10335-9

Cited by 8 publications

(3 citation statements)

References 57 publications

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“…PPP1R3G deletion accelerates the metabolic rate and reduces the glycogen level in adipose tissue in mice, indicating that PPP1R3G links glycogen and lipid metabolisms in vivo ( 29 ). In fish (silver carp Hypophthalmichthys molitrix ), a study found that after a treatment with toxic Microcystis aeruginosa , PPP1R3G was down-regulated, indicating that it might play a major role in the detoxifying and antitoxic mechanisms of microcystin in fish ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Wang

Feng

et al. 2021

Front. Immunol.

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Protein phosphatase-1 (PP1) has an important role in many cell functions, such as cell differentiation, development, immune response and tumorigenesis. However, the specific role of PP1 in the antiviral response in fish remains to be elucidated. In this study, the PPP1R3G homolog was identified in the grass carp (Ctenopharyngodon idella) and its role in defence against the GCRV infection was investigated. Phylogenetic analysis demonstrated that CiPPP1R3G clustered with homologues from other teleosts. Temporal expression analysis in vivo revealed that the expression level of CiPPP1R3G was significantly up-regulated in response to GCRV infection in grass carps, especially in the intestine and head-kidney. Cellular distribution analysis revealed that CiPPP1R3G was located in the nucleus and cytoplasm. Overexpression of CiPPP1R3G significantly negatively regulated the expression of CiIRF3, thus inhibiting its activation. In summary, we systematically analyzed the PPP1R3G gene in grass carp and illustrated its function as a negative regulator in the anti-GCRV immune responses.

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Section: Discussionmentioning

confidence: 99%

Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Wang

Feng

et al. 2021

Front. Immunol.

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“…It has been proved that oral administration of MCs to fish led to hepatic necrosis and death (Tencalla et al, 1994). Hu et al (2017) evaluated the effects of M. aeruginosa toxicity on Hypophthalmichtys molitrix (silver carp) by using hepatic RNAseq and miRNA-seq. Furthermore, it has been reported that M. aeruginosa alters some biochemical markers of Oreochromis niloticus (Marzouk et al, 2013).…”

Section: Impacts Of M Aeruginosa On Other Living Organismsmentioning

confidence: 99%

Toxicological Studies on Microcystin Produced by Microcystis aeruginosa : Assessment and Management

Khairy

El‐Sheekh

2019

Egypt. J. Bot.

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ICROCYSTIS aeruginosa is a kind of cyanobacteria microorganism that synthesizes and produces peptides, which could be highly toxic. The most common toxin known as microcystin (MCs) or cyanotoxins, these toxins are heptapeptides produced by cyanobacterial blooms on water surfaces. They potentially lead to acute to chronic health-related problems via increasing reactive oxygen species (ROS) and irreversible inhibition of serine/threonine protein phosphatases. The toxicological studies of MCs on experimental animal and cell models have promise interests; however, few information is known about their impacts on humans due to acute or chronic exposure. This review article aimed to present some of the information about the cyanotoxins structure, occurrence, and distribution of the cyanobacterial toxins especially excreted from M. aeruginosa. Also, toxicological studies and investigations associated with M. aeruginosa producing microcystin, their assessments and control and health problems and cancer risk upon MCs exposure will be discussed. The article will also discuss the mechanism of the toxin and the way for control and degradation.

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“…In addition, another study reported that the biological relationship between PPP1R3G and Sorcin could be related to the calcium, glucose, and lipid homeostasis [ 10 ]. A study also found that the PPP1R3G was downregulated after treatment with toxic Microcystis aeruginosa, suggesting that it plays a critical role in detoxification, along with the antitoxic mechanisms of microcystin in fish [ 11 ]. Researchers found that PPP1R3G was upregulated in transcripts after GCRV infection in grass carp, which indicated that PPP1R3G plays a negative role in anti-GCRV immune responses [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Protein phosphatase 1 regulatory subunit 3G (PPP1R3G) correlates with poor prognosis and immune infiltration in lung adenocarcinoma

et al. 2021

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The protein phosphatase 1 regulatory subunit 3G (PPP1R3G) participates in many tumor biological processes; however, its effects on lung adenocarcinoma (LUAD) have not been clarified. Therefore, this study aimed to explore the correlation between PPP1R3G and the prognosis and immune invasion of LUAD. We evaluated the relationship between PPP1R3G and LUAD using a wide range of databases and analysis tools, including UALCAN, TIMER, miRDB, The Human Protein Atlas and the MethSurv database. First, we explored the mRNA and protein expression levels of PPP1R3G in LUAD, and results were validated using real-time PCR. Next, we explored the relationship between PPP1R3G expression and clinical features. Finally, Kaplan-Meier curves and Cox regression were employed to investigate the prognostic significance of PPP1R3G in LUAD. In addition, we explored the relationship between the expression of PPP1R3G and immune infiltration using the TIMER database. We analyzed the relationship between PPP1R3G and methylation using MethSurv database. Results showed that PPP1R3G expression in LUAD tissues was higher than that in normal tissues, and high expression was suggestive of a poor prognosis. Moreover, PPP1R3G expression was positively correlated with the immune infiltration of CD4+ T cells, macrophages, neutrophils, and dendritic cells. PPP1R3G copy number variations also demonstrated remarkable associations with the levels of B cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells. Finally, a PPP1R3G-associated regulatory network was constructed. Overall, PPP1R3G might be a poor prognostic biomarker for LUAD and is associated with tumor immune cell infiltration.

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Effects of toxic Microcystis aeruginosa on the silver carp Hypophthalmichtys molitrix revealed by hepatic RNA-seq and miRNA-seq

Cited by 8 publications

References 57 publications

Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Toxicological Studies on Microcystin Produced by Microcystis aeruginosa : Assessment and Management

Protein phosphatase 1 regulatory subunit 3G (PPP1R3G) correlates with poor prognosis and immune infiltration in lung adenocarcinoma

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