Effects of Tramadol on the Development of Lucilia sericata (Diptera: Calliphoridae) and Detection of the Drug Concentration in Postmortem Rabbit Tissues and Larvae

El-Samad, Lamia M.; El-Moaty, Zeinab A.; Makemer, Hassan M.

doi:10.3923/je.2011.353.364

Cited by 23 publications

(12 citation statements)

References 7 publications

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“…In our study, tramadol treated carcasses decayed with the same rate as the control carcass except in winter season which slightly delayed. This result was agreeable with El-Samad et al (2011), Ekrakene and Odo (2017) and Hamdy et al (2022). However, in contrast with our result, AbouZied (2016) found that the decomposition process of tramadol treated rabbits was slightly faster than that of control group; however he observed also that the total decomposition rate of the tramadol-treated corpses was not significantly faster than the control ones during his study period in spring season.…”

Section: Discussionsupporting

confidence: 91%

Comparative Study on Forensic Insects encountered on Rabbit Corpses killed with different methods in Upper Egypt

Aly

Osman²,

Ali³

et al. 2023

SVU-International Journal of Veterinary Sciences

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This work aims to compare between the forensic insect species, their succession patterns and decomposition stages of rabbit corpses killed with different methods in Qena city, Egypt. Four experiments were carried out during this study for four successive seasons. Four rabbits were placed in each experiment, the first two rabbits received a double LD50 of tramadol hydrochloride and cypermethrin via ear vein injection. The third rabbit was slaughtered while the fourth rabbit was euthanized with air injection (control). The LD50 of tramadol and cypermethrin in male rabbit was calculated from logarithmic scale and found to be 132 mg/kg and 126 µ|/kg body weight, respectively.Currently, a total of 14271 insect individuals were collected from the screened bodies belonging to three orders, thirteen families and seventeen species. Moreover, corpses were colonized by different insect life stages (11689, 352 and 2230 individuals) included dipteran flies, Coleopteran and Hymenopteran insects, respectively The total number of species collected in summer was lower than in other seasons. Tramadol-treated rabbits decayed with similar rate to the control corpses during all seasons except in winter. As well as, decomposition process was significantly prolonged in cypermethrin killed rabbits during all seasons. However, slaughtered carrions decayed faster than control in winter and spring. Maggots deposited on cypermethrin-intoxicated carrions were very few and have been dead after few days from oviposition. Density of maggots collected from control corpse was loweras compared to the slaughtered one and higher than that on tramadol treated corpse. Therefore, these results are recommended to be taken in criminal investigations.

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Section: Discussionsupporting

confidence: 91%

Comparative Study on Forensic Insects encountered on Rabbit Corpses killed with different methods in Upper Egypt

Aly

Osman²,

Ali³

et al. 2023

SVU-International Journal of Veterinary Sciences

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“…In the lungs, muscles, and fat, the concentration of AlP was lower. Similar results were obtained by El-Samad et al, 2011 31 , who detected tramadol by HPLC in various rabbits' organs experimentally injected with this substance. Importantly, AlP concentration in rabbit tissues was signi cantly higher than in the body of the third instar of C. albiceps.…”

Section: Discussionsupporting

confidence: 89%

“…Toxins also affect the growth rate of y larvae developing on the corpses. Many studies have assessed the impact of drugs/pesticides on the morphometry of y species 35,36 and their development 31,37,38 in terms of forensic entomotoxicology. The vast majority of drugs accelerate the development of larvae, while pesticides tend to delay it.…”

Section: Discussionmentioning

confidence: 99%

“…Elshehaby et al, 2019 46 found that tramadol retards larval development of C. albiceps. Also, El-Samad et al, 2011 31 demonstrated that Lucilia sericata larvae reared on rabbits administered tramadol had a prolonged developmental period. Whereas Ekrakene and Odo (2017) 47 established that the larvae from the tramadol reared group gained body length and weight better than the control groups.…”

Section: Discussionmentioning

confidence: 99%

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Reduced body length and morphological disorders in Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae) larvae reared on aluminum phosphide-treated rabbits

El‐Ashram¹,

Na²,

Ae³

et al. 2021

Preprint

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Assessing the post-mortem interval (PMI) based on the growth and development of insects is a critical task in forensic entomology. The rate of larvae development can be affected by a variety of toxins, including pesticides. Aluminum phosphide (AlP) is a low-cost insecticide that has yet to be entomotoxicologically tested, despite the fact that it is frequently the cause of fatal poisoning. In this study, we measured the body length of Chrysomya albiceps larvae reared on the carcasses of rabbits poisoned with AlP and analyzed the morphological changes of the larvae reared on the carcasses of rabbits poisoned with AlP. The concentration of AlP in the body of the larvae was significantly lower than in rabbit tissues. Insects from the AlP group had a significantly lower gain in body length. Furthermore, deformities in the larvae were found. Small respiratory spiracles were found, as well as a deformed small posterior end with hypogenesis of the posterior respiratory spiracles. Thus, disturbed growth and development of carrion flies found at a crime scene could indicate pesticide poisoning, such as aluminum phosphide.

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“…Tantawi et al (2001) utilized a radioimmunoassay technique to measure the level of morphine in the 3rd instar larvae of blowflies and flesh flies attracted to rabbit carcasses left outdoors. El-Samad et al (2011) employed high-performing liquid chromatography with a UV-visible spectrophotometric detector to identify tramadol in rabbit blood and tissues, along with fly larvae, to assess tramadol's impact on the growth of L. sericata larvae. According to the research, tramadol concentrations in larvae were much lower than those detected in tissues, treated rabbits reached maximum size faster than control rabbits, and the overall developing period rose significantly when the first administered dose was increased.…”

Section: Entomotoxicologymentioning

confidence: 99%

Forensic entomology research in Egypt: a review article

Mashaly

Ibrahim

2022

Egypt J Forensic Sci

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Background Forensic entomology (FE) is widely considered as an important field of forensic science, particularly in the field of estimating postmortem intervals, which has advanced considerably across the world. Main body Researchers used necrophagous insects in twelve Egyptian governorates to look into the variables that impact insect succession and the growth of juvenile stages from infesting animal models and humans, draw toxicological conclusions, and identify insect species using morphological and molecular-based approaches. The study includes analyses of insect abundance and dispersion, along with biochemical and electrophysiological experiments. Comparable data from other Egyptian governorates, on the other side, is currently unavailable. Aspects of FE are also missing, which might be addressed in a future research project. Conclusion This review aims to provide an outline of FE in Egypt, other than to assist existing researchers in identifying research needs and recruiting new researchers into the field by highlighting significant forensic insects that might be employed in criminal prosecutions.

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Effects of Tramadol on the Development of Lucilia sericata (Diptera: Calliphoridae) and Detection of the Drug Concentration in Postmortem Rabbit Tissues and Larvae

Cited by 23 publications

References 7 publications

Comparative Study on Forensic Insects encountered on Rabbit Corpses killed with different methods in Upper Egypt

Comparative Study on Forensic Insects encountered on Rabbit Corpses killed with different methods in Upper Egypt

Reduced body length and morphological disorders in Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae) larvae reared on aluminum phosphide-treated rabbits

Forensic entomology research in Egypt: a review article

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