Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria

Becker, Regina; Behrendt, Undine; Hommel, Bernd; Kropf, Siegfried; Ulrich, Andreas

doi:10.1111/j.1574-6941.2008.00562.x

Cited by 41 publications

(27 citation statements)

References 72 publications

(101 reference statements)

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“…The high abundance of Acinetobacter sp. in a potato phytosphere has also been reported in a series of previous studies (3, 43, 52). These studies demonstrated that Acinetobacter spp.…”

Section: Discussionsupporting

confidence: 79%

Molecular Characterization of the Bacterial Community in a Potato Phytosphere

et al. 2013

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The bacterial community of a potato phytosphere at the flowering stage was examined using both culture-dependent and -independent methods. Tissues (leaves, stems, roots and tubers) were sampled from field-grown potato plants (cultivar Matilda), and the clone libraries of 16S rRNA genes and the isolate collections using R2A medium were constructed. By analyzing the combined data set of 16S rRNA gene sequences from both clone libraries and isolate collections, 82 genera from 8 phyla were found and 237 OTUs (≥97% identity) at species level were identified across the potato phytosphere. The statistical analyses of clone libraries suggested that stems harbor the lowest diversity among the tissues examined. The phylogenetic analyses revealed that the most dominant phylum was shown to be Proteobacteria for all tissues (62.0%–89.7% and 57.7%–72.9%, respectively), followed by Actinobacteria (5.0%–10.7% and 14.6%–39.4%, respectively). The results of principal coordinates analyses of both clone libraries and isolate collections indicated that distinct differences were observed between above- and below-ground tissues for bacterial community structures. The results also revealed that leaves harbored highly similar community structures to stems, while the tuber community was shown to be distinctly different from the stem and root communities.

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“…The high abundance of Acinetobacter sp. in a potato phytosphere has also been reported in a series of previous studies (3, 43, 52). These studies demonstrated that Acinetobacter spp.…”

Section: Discussionsupporting

confidence: 79%

Molecular Characterization of the Bacterial Community in a Potato Phytosphere

et al. 2013

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“…Evaluating these effects in the context of natural cultivar variability showed that changes in the composition of bacterial and fungal communities associated with GM plants were not greater than differences among cultivars. Moreover, the GM effects were negligible compared to the effects of environmental factors, such as field site or year, which were far greater than the effects of genetic modification or plant genotype (2,20,30,38).…”

Section: Discussionmentioning

confidence: 96%

“…However, potential effects of GM plants on microbial communities need to be evaluated in light of natural variation among cultivars of the same plant species. Recently, a study of the rhizosphere communities of fructanproducing GM potatoes compared to those of isogenic controls and conventional cultivars failed to show plant genotype effects (2). However, this result was based only on analysis of Bacteria and did not consider potential effects on different microbial groups.…”

mentioning

confidence: 99%

Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Weinert

Meincke

Gottwald

et al. 2009

Appl Environ Microbiol

126

111

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The effects of genetically modified (GM), zeaxanthin-accumulating potato plants on microbial communities in the rhizosphere were compared to the effects of different potato cultivars. Two GM lines and their parental cultivar, as well as four other potato cultivars, were grown in randomized field plots at two sites and in different years. Rhizosphere samples were taken at three developmental stages during plant growth and analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprints of Bacteria, Actinobacteria, Alpha-and Betaproteobacteria, Bacillus, Streptomycetaceae, Pseudomonas, gacA, Fungi, and Ascomycetes. In the bacterial DGGE gels analyzed, significant differences between the parental cultivar and the two GM lines were detected mainly for Actinobacteria but also for Betaproteobacteria and Streptomycetaceae, yet these differences occurred only at one site and in one year. Significant differences occurred more frequently for Fungi, especially Ascomycetes, than for bacteria. When all seven plant genotypes were compared, DGGE analysis revealed that different cultivars had a greater effect on both bacterial and fungal communities than genetic modification. The effects of genetic modification were detected mostly at the senescence developmental stage of the plants. The site was the overriding factor affecting microbial community structure compared to the plant genotype. In general, the fingerprints of the two GM lines were more similar to that of the parental cultivar, and the differences observed did not exceed natural cultivar-dependent variability.

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“…Fortunately, this effect is temporary and its duration is correlated with the duration of γ-lactone assimilation [18,85]. Other changes in the microflora structure have also been recorded [85,89], but they do not appear to be greater than those observed during the phase change associated with plant growth, experimental culture conditions (field and year), climatic variations or cultural practices [56,[109][110][111][112].…”

Section: Discussionmentioning

confidence: 92%

Rhodococcus erythropolis and Its γ-Lactone Catabolic Pathway: An Unusual Biocontrol System That Disrupts Pathogen Quorum Sensing Communication

et al. 2013

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Abstract:Rhodococcus erythropolis is an environmental Gram-positive Actinobacterium with a versatile metabolism involved in various bioconversions and degradations. Rhodococci are best known for their great potential in numerous decontamination and industrial processes. However, they can also prevent plant disease by disrupting quorum sensing-based communication of Gram-negative soft-rot bacteria, by degrading N-acyl-homoserine lactone signaling molecules. Such biocontrol activity results partly from the action of the γ-lactone catabolic pathway. This pathway is responsible for cleaving the lactone bond of a wide range of compounds comprising a γ-butyrolactone ring coupled to an alkyl or acyl chain. The aliphatic products of this hydrolysis are then activated and enter fatty acid metabolism. This short pathway is controlled by the presence of the γ-lactone, presumably sensed by a TetR-like transcriptional regulator, rather than the presence of the pathogen or the plant-host in the environment of the Rhodococci. Both the density and biocontrol activity of R. erythropolis may be boosted in crop systems. Treatment with a cheap γ-lactone stimulator, for example, the food flavoring γ-caprolactone, induces the activity in the biocontrol agent, R. erythropolis, of the pathway degrading signaling molecules; such treatments thus promote plant protection.

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Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria

Cited by 41 publications

References 72 publications

Molecular Characterization of the Bacterial Community in a Potato Phytosphere

Molecular Characterization of the Bacterial Community in a Potato Phytosphere

Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Rhodococcus erythropolis and Its γ-Lactone Catabolic Pathway: An Unusual Biocontrol System That Disrupts Pathogen Quorum Sensing Communication

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