Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation

Suksai, Manaphat; Dhanaworavibul, Kriengsak

doi:10.31584/jhsmr.201945

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…As these sugars are non-permeable cryoprotectants, they cover the outer layer surface of the sperm membrane, stabilize, and protect the cell membrane structure. Only a few studies have investigated the effects of trehalose on human sperm cryopreservation [ 17 , 20 , 43 ]. Moreover, to the best of our knowledge, no studies have been performed on the impact of gentiobiose on sperm cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…Trehalose might positively affect some sperm parameters, such as sperm count, percent motility, normal morphology, and vitality [ 20 , 43 ]. So far, no study has been observed on the effects of gentiobiose on these parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Also, 0.025, 0.05, 0.1 and 0.2 M gentiobiose solution were separately added to each medium. Then, 200 μM Salidroside was added to the medium and stored at -20°C for the next experiment [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation

et al. 2023

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The protection of human sperm during cryopreservation is of great importance to infertility. Recent studies have shown that this area is still a long way from its ultimate aim of maintaining the maximum viability of sperm in cryopreservation. The present study used trehalose and gentiobiose to prepare the human sperm freezing medium during the freezing-thawing. The freezing medium of sperm was prepared with these sugars, and the sperm were then cryopreserved. The viable cells, sperm motility parameters, sperm morphology, membrane integrity, apoptosis, acrosome integrity, DNA fragmentation, mitochondrial membrane potential, reactive oxygen radicals, and malondialdehyde concentration was evaluated using standard protocols. A higher percentage of the total and progressive motility, rate of viable sperm, cell membrane integrity, DNA and acrosome integrity, and mitochondrial membrane potential were observed in the two frozen treatment groups compared to the frozen control. The cells had less abnormal morphology due to treatment with the new freezing medium than the frozen control. The higher malondialdehyde and DNA fragmentation were significantly observed in the two frozen treatment groups than in the frozen control. According to the results of this study, the use of trehalose and gentiobiose in the sperm freezing medium is a suitable strategy for sperm freezing to improve its motion and cellular parameters.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation

et al. 2023

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Impacts of adding sucrose or trehalose to extenders with different glycerol concentrations on freezablility and fertility of buffalo bull semen

Khalil,

El-Deghaidy,

Sakr

et al. 2024

Vet Res Commun

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This experiment was conducted to determine the most suitable glycerol concentration (3 or 6%) and/or non-penetrating cryoprotectants (trehalose and sucrose) for the cryopreservation of buffalo semen, with the aim of enhancing the cryopreservation protocol. Semen of Egyptian buffalo were pooled and diluted with eight Tris extenders supplemented with either 6% glycerol (control group, GL6), 3% (low level, GL3), sucrose (SU, 50 mM), trehalose (TR, 50 mM), 6% glycerol together with 50 mM of sucrose (GL6SU) or 50 mM of trehalose (GL6TR), and 3% of glycerol together with 50 mM of sucrose (GL3SU) or 50 mM of trehalose (GL3TR), then frozen following the standard protocol. Findings indicated that GL3 extender resulted in the highest values of progressive motility, sperm kinematics, sperm membrane integrity, and viability of post-thawed semen (37 °C for 30 s). On the contrary, the Tris extender enriched only with SU and TR groups had the lowest values of sperm quality compared to the other groups (p < 0.05). All GL supplemented groups showed higher intact acrosome levels and lower detached acrosome and dead sperm with intact acrosome compared to those with TR and SU alone (p < 0.05). A significant increase in viable sperm was observed in the GL3, GL6, and GL3SU groups compared to the other groups (p < 0.05). The Tris extender supplemented with low glycerol (3%) significantly reduced the levels of MDA. In the in vivo fertility trial, it was shown that the pregnancy rate was higher in the GL6SU group (72%) than in the GL3SU group (68%; p > 0.05). Collectively, these results suggest that there is potential in using low glycerol (3%) as a cryoprotective agent in the medium for buffalo sperm cryopreservation without significant adverse effects compared to the addition of 6% glycerol. This study supported the sustainability of materials used in assisted reproductive technology by reducing the glycerol content in the freezing medium. Further research is needed to confirm this hypothesis.

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Tris and Trehalose based modified cryopreservation dilutor in block freezing improves cryo-survivability and functionality of Sahiwal bull semen

Sethi,

Mohanty,

Shah

et al. 2024

Preprint

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Our study aimed to enhance cryoprotection and improve post-thaw sperm quality by using a new freezing protocolthat combines trehalose and glycerol.The semen ejaculate from each bull was collected and split into three aliquots.Split one was the control group (C), in which semen was extended in a tris fructose egg yolk glycerol (TFEG) extender and underwent the conventional freezing protocol.In split two (T1) and split three (T2) groups, the semen was diluted in tris fructose egg yolk extender containing 25% trehalose, with glycerol 5% in both groups. Split two (T1) underwent conventional freezing, whereas split three (T2) underwent aluminium block freezing.After freezing-thawing, the progressive sperm motility,viability, acrosome integrity, sperm velocity and path parameters were significantly (p ≤ 0.05) improved in T2 among groups. Additionally, the degree of oxidative stress was substantially lower in T2 among groups.The present study's findings revealed the promising role of trehalose (25%) and glycerol (5%) in tris-based extender in achieving aluminium block freezing, as it significantly improved the post-thaw sperm survivability, and this method can suitably be used for quality frozen semen production.

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Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation

Cited by 3 publications

References 15 publications

Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation

Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation

Impacts of adding sucrose or trehalose to extenders with different glycerol concentrations on freezablility and fertility of buffalo bull semen

Tris and Trehalose based modified cryopreservation dilutor in block freezing improves cryo-survivability and functionality of Sahiwal bull semen

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