Effects of TRH and TRH-like peptide pGLU-HIS-GLY-NH2 on adrenocortical cell proliferation in rats

Zerek-Mełeń, G; Pawlikowski, Marek

doi:10.1007/bf02738704

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…Relatively high doses of TRH (1 ng/ml) are unable to stimulate DNA synthesis or even to slightly decrease (10 ng/ml TRH) the number of BrdU-labeled cells, although no statistical difference has been noted (P= 0.094). Previous studies have shown that TRH and/or TRH-like peptides can directly or indirectly stimulate the proliferation of thymus cells, anterior pituitary cells, and adrenocortical cells (Pawlikowski and Slowinska-Klencka 1994;Pawlikowski et al 1992;Zerek-Mełeń 1996). Recently, TRH has been found potentially to maintain and stimulate thyrotroph abundance in aggregate cell cultures of postnatal rat pituitary gland (Pals et al 2006).…”

Section: Discussionmentioning

confidence: 95%

Expression of thyrotropin-releasing hormone receptors in rat testis and their role in isolated Leydig cells

et al. 2008

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Thyrotropin-releasing hormone (TRH) was initially discovered as a neuropeptide synthesized in the hypothalamus. Receptors for this hormone include TRH-receptor-1 (TRH-R1) and -2 (TRH-R2). Previous studies have shown that TRH-R1 and TRH-R2 are localized exclusively in adult Leydig cells (ALCs). We have investigated TRH-R1 and TRH-R2 expression in the testes of postnatal 8-, 14-, 21- 35-, 60-, and 90-day-old rats and in ethane dimethane sulfonate (EDS)-treated adult rats by using Western blotting, immunohistochemistry, and immunofluorescence. The effects of TRH on testosterone secretion of primary cultured ALCs from 90-day-old rats and DNA synthesis in Leydig cells from 21-day-old rats have also been examined. Western blotting and immunohistochemistry demonstrated that TRH-R1 and TRH-R2 were expressed in fetal Leydig cells (in 8-day-old rats) and in all stages of adult-type Leydig cells during development. Immunofluorescence double-staining revealed that newly regenerated Leydig cells in post-EDS 21-day rats expressed TRH-R1 and TRH-R2 on their first reappearance. Incubation with various doses of TRH affected testosterone secretion of primary cultured ALCs. Low concentrations of TRH (0.001, 0.01, and 0.1 ng/ml) inhibited basal and human chorionic gonadotrophin (hCG)-stimulated testosterone secretion of isolated ALCs, whereas relatively high doses of TRH (1 and 10 ng/ml) increased hCG-stimulated testosterone secretion. As detected by a 5-bromo-2'-deoxyuridine incorporation test, the DNA synthesis of Leydig cells from 21-day-old rats was promoted by low TRH concentrations. Thus, we have clarified the effect of TRH on testicular function: TRH might regulate the development of Leydig cells before maturation and the secretion of testosterone after maturation.

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Section: Discussionmentioning

confidence: 95%

Expression of thyrotropin-releasing hormone receptors in rat testis and their role in isolated Leydig cells

et al. 2008

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Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat

Joustra¹,

Meijer²,

Heinen³

et al. 2015

Journal of Endocrinology

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Loss-of-function mutations in the immunoglobulin superfamily member 1 (IGSF1) gene cause an X-linked syndrome of central hypothyroidism, macroorchidism, variable prolactin and GH deficiency, delayed pubertal testosterone rise, and obesity. To understand the pathophysiology of this syndrome, knowledge on IGSF1's place in normal development is imperative. Therefore, we investigated spatial and temporal protein and mRNA expression of IGSF1 in rats using immunohistochemistry, real-time quantitative PCR (qPCR), and in situ hybridization. We observed high levels of IGSF1 expression in the brain, specifically the embryonic and adult choroid plexus and hypothalamus (principally in glial cells), and in the pituitary gland (PIT1-lineage of GH, TSH, and PRL-producing cells). IGSF1 is also expressed in the embryonic and adult zona glomerulosa of the adrenal gland, islets of Langerhans of the pancreas, and costameres of the heart and skeletal muscle. IGSF1 is highly expressed in fetal liver, but is absent shortly after birth. In the adult testis, IGSF1 is present in Sertoli cells (epithelial stages XIII-VI), and elongating spermatids (stages X-XII). Specificity of protein expression was corroborated with Igsf1 mRNA expression in all tissues. The expression patterns of IGSF1 in the pituitary gland and testis are consistent with the pituitary hormone deficiencies and macroorchidism observed in patients with IGSF1 deficiency. The expression in the brain, adrenal gland, pancreas, liver, and muscle suggest IGSF1's function in endocrine physiology might be more extensive than previously considered.

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Effects of TRH and TRH-like peptide pGLU-HIS-GLY-NH2 on adrenocortical cell proliferation in rats

Cited by 2 publications

References 17 publications

Expression of thyrotropin-releasing hormone receptors in rat testis and their role in isolated Leydig cells

Expression of thyrotropin-releasing hormone receptors in rat testis and their role in isolated Leydig cells

Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat

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