Effects of triclosan on antioxidant- and apoptosis-related genes expression in the gill and ovary of zebrafish

Wang, Fan; Zheng, Fang; Liu, Fei

doi:10.1538/expanim.19-0115

Cited by 28 publications

(16 citation statements)

References 46 publications

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“…GR plays an important role along with GPx for maintenance of redox potential and is responsible for regeneration of glutathione during detoxi cation of peroxides and free radicals. Decline in the expression of both these genes after 96h exposure is supported by earlier studies which also reported a declined expression of Phospholipid hydroperoxide glutathione peroxidase (PHGPx) in liver of tadpole Bufo gargarizans (Chai et al 2017), GPX4b in male juvenile zebra sh (Wang et al 2019a), GPX1a in gill and ovary of zebra sh (Wang et al 2019b). Signi cant inhibition of GR expression in the TCS exposed larvae in the present study is corroborated by the nding of Falisse et al (2017) and Gyimah et al (2020) who also reported declined GR activity in zebra sh early life stages and in the liver of adult zebra sh, respectively.…”

Section: Discussionsupporting

confidence: 58%

“…At lower levels, ROS have been shown to activate the pathways that reinforce defense responses and enhance survival to subsequent oxidative stress (Sanchez et al 2015 (Song et al 2006;Sun et al 2009). Similar pattern of declined SOD expression has also been reported in male juvenile zebra sh (Wang et al 2019a), gill and ovary of zebra sh (Wang et al 2019b) and liver of tadpole Bufo gargarizans (Chai et al 2017) after TCS exposure. The decrease during exposure in the present study was followed by an increase in expression of all the genes during the recovery period but the increase was 2-5 folds more than control.…”

Section: Discussionsupporting

confidence: 54%

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Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Environ Sci Pollut Res

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Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous sh, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxi cation (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused signi cant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased signi cantly (except for a signi cant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined signi cantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionsupporting

confidence: 54%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Environ Sci Pollut Res

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“…But limonin treatment reduced the accumulation of ROS and partially restored GSH. Besides, gpx1a has been confirmed to have an antioxidant effect in NAFLD ( Wang et al, 2020 ). Our results showed that limonin could reverse the decrease in gpx1a caused by TAA.…”

Section: Discussionmentioning

confidence: 99%

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Yang

Lin

et al. 2022

Front. Pharmacol.

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Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and continues to rise in the worldwide. Limonin is a triterpenoid compound widely found in the fruits of citrus plants with a wide range of pharmacological effects, including anti-cancer, anti-inflammation, anti-viral, anti-oxidation and liver protection properties. However, the potential molecular mechanism of limonin on NAFLD in zebrafish remains unknown. In this study, zebrafish larvae were exposed to thioacetamide to establish an NAFLD model and the larvae were treated with limonin for 72 h simultaneously. The human liver cell line was stimulated with lipid mixture and meanwhile incubated with limonin for 24 h. The results showed that Limonin significantly reduced the accumulation of lipid droplets in the liver and down-regulated the levels of lipogenic transcription factors FASN and SREBP1 in NAFLD. Limonin suppressed macrophages infiltration and the down-regulated the relative expression levels of the pro-inflammatory factors IL-6, IL-1β and TNF-α secreted by macrophages. Besides, limonin could reversed the reduction of glutathione and the accumulation of reactive oxygen species through up-regulating NRF2/HO-1 signaling pathway in the liver. In conclusion, this study revealed that limonin has a protective effect on NAFLD due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions.

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“…The effects of TCS exposure on the expression of antioxidant (SOD, GPx1a, CAT, sMT-B, and MT-2) and apoptosis-related genes (Bax, p53) in zebrafish ovarian tissue were investigated [26], and it was shown that the antioxidant-related genes in the ovary were significantly downregulated and apoptotic-related genes were significantly upregulated in groups exposed to TCS. The authors stated that TCS exposure caused oxidative damage in the ovaries and induced reactive oxygen species (ROS)-dependent apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan

Akbulut

2021

ARCH BIOL SCI BELGRA

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Triclosan, produced as a broad-spectrum antibiotic in the early 1960s, is generally used as a preservative in personal care products, fabrics, plastic products such as kitchenware and toys. As a result of the high demand for triclosan, this chemical threatens the aquatic ecosystem by contaminating wastewater sources. Environmental pollutants affect the reproductive potential of fish, one of the most critical aquatic organisms. This study aimed to investigate the histopathological and apoptotic effects of triclosan in zebrafish gonads. Fish were exposed to sublethal concentrations of triclosan for 5 days, and general histological methods were applied. Histological sections were examined under a light microscope after staining with hematoxylin and eosin and toluidine blue. Triclosan exposure caused deterioration in ovarian tissue, such as shrinkage in the ooplasm, accumulation of proteinaceous fluid in the interstitial tissue, morphological changes of oocyte and the zona radiata. In testicular tissue, triclosan exposure caused fusion in seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, edema in seminiferous tubules, and karyorrhexis in spermatogenic cells. The TUNEL assay was used for the determination of apoptotic cells. Brown-colored apoptotic cells were visualized under the light microscope. TUNEL positive cells were observed in all exposure groups. Triclosan administration was found to cause apoptosis in zebrafish gonads. These findings indicate that triclosan potentially affects fish reproduction, and that its judicious disposal is essential for protecting the environment and maintaining the reproductive potential of fish.

show abstract

Effects of triclosan on antioxidant- and apoptosis-related genes expression in the gill and ovary of zebrafish

Cited by 28 publications

References 46 publications

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan

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