Effects of trifluoperazine and verapamil on the hydro-osmotic response to antidiuretic hormone in the urinary bladder of the toad

Kipnowski, J.; Düsing, Rainer; Kramer, Herbert J.

doi:10.1007/bf01851916

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…It was reported recently that verapamil inhib ited ADH-stimulated water flow in the toad urinary bladder [15,16]. Considering the data, the increased CFDO in the present experiment may be conceivable.…”

Section: Discussionmentioning

confidence: 46%

Unilateral Renal Arterial Infusion and Renal Vein Catheterization in Rabbits

Cho¹,

Koh²,

Kim³

et al. 1987

Kidney Blood Press Res

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This study evaluates the direct effects of verapamil and furosemide infused into the unilateral renal artery on renal function and the renin secretion rate in renal vein-catheterized rabbits. Catheterization did not alter the renal function parameters of the kidney. Verapamil and furosemide increased renal blood flow, urine flow, and urinary sodium, potassium and chloride excretions confined to the infused kidney. Verapamil increased the glomerular filtration rate and free water clearance. The renin secretion rate was increased by furosemide but not by verapamil. The present study shows that the technique is applicable to renal function studies in which unilateral renal arterial infusion of the agents studied is required. The contralateral kidney can be a reliable control for the infused kidney. It also provides a useful technique for the study of renin release in rabbits.

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Section: Discussionmentioning

confidence: 46%

Unilateral Renal Arterial Infusion and Renal Vein Catheterization in Rabbits

Cho¹,

Koh²,

Kim³

et al. 1987

Kidney Blood Press Res

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show abstract

Effects of standard diuretics and RPH 2823 on transepithelial Na+ transport in isolated frog skin

et al. 1986

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Short-circuit current (SCC) techniques were used to monitor the effects of various diuretic agents on Na+ transport in isolated frog skin, a model for the late distal tubule and the collecting duct of the mammalian kidney. Acetazolamide, hydrochlorothiazide, torasemide, and ethacrynic acid did not affect sodium transport (as indicated by the SCC) or transepithelial electrical resistance when added either to the apical (outer) or to the inner (basolateral, corial) bathing solution of the tissue. However, Na+ transport was sensitive to amiloride, the triamterene derivate dimethylamino-hydroxypropoxytriamterene (RPH 2823), and to furosemide. Whereas apical amiloride, and RPH 2823 induced a dose-dependent decrease in SCC and increase in transepithelial electrical resistance, apical furosemide resulted in a dose-dependent increase in SCC and a decrease in electrical resistance. None of the three diuretic agents caused a significant change in SCC when applied to the inner bathing Ringer's solution. The small furosemide-induced decrease in resistance compared with the huge increase in SCC suggests that furosemide affects Cl- permeability as well as Na+ permeability. Evidence for this notion was achieved by the following findings: The decrease in resistance after furosemide was more pronounced in tissues bathed in Cl(-)-free solutions compared with Cl(-)-containing solutions. n contrast, SCC stimulation by apical furosemide is Cl(-)-ion independent, but strongly Na+-ion dependent. SCC stimulation by furosemide is amiloride-sensitive. With respect to the onset, locus, and reversibility of action, it seems reasonable to assume that amiloride, RPH 2823, and furosemide all influence transepithelial Na+ transport by interacting with the Na+ channel or a regulator site of it within the apical membrane. The stoichiometry of the amiloride (RPH 2823)-receptor site interaction revealed Hill-coefficient(s) of less than 1, indicating a negative cooperativity among the receptor sites. The interaction between Na+ ions and amiloride or RPH 2823 displayed mixed competitive-noncompetitive inhibition. Taken together, these results support the hypothesis that amiloride and Na+ as well as RPH 2823 and Na+ may act at different loci on the apical entry mechanism in Rana esculenta skin.

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Effects of trifluoperazine and verapamil on the hydro-osmotic response to antidiuretic hormone in the urinary bladder of the toad

Cited by 2 publications

References 19 publications

Unilateral Renal Arterial Infusion and Renal Vein Catheterization in Rabbits

Unilateral Renal Arterial Infusion and Renal Vein Catheterization in Rabbits

Effects of standard diuretics and RPH 2823 on transepithelial Na+ transport in isolated frog skin

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