Effects of trophic exposure to dexamethasone and diclofenac in freshwater fish

Guiloski, Izonete Cristina; Ribas, João Luiz Coelho; Pereira, Letícia da Silva; Neves, Ana; Assis, Helena C. Silva de

doi:10.1016/j.ecoenv.2014.11.020

Cited by 111 publications

(55 citation statements)

References 52 publications

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“…Doses of anti-inflammatory drugs were based on preliminary work with this fish model [9]. Fish were maintained at 24 AE 1 8C in glass aquaria (120-L capacity) in filtered and dechlorinated tap water with a 12:12-h light:dark photoperiod for 30 d. After acclimation to laboratory conditions, fish were randomly divided into 4 groups with n ¼ 20.…”

Section: Experimental Designmentioning

confidence: 99%

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Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish

Ribas

Zampronio

Assis

2015

Enviro Toxic and Chemistry

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The aim of the present study was to evaluate the effects of diclofenac and dexamethasone on hematological parameters and immune response in the fish species Hoplias malabaricus after trophic exposure. Fish were fed twice every week with Astyanax sp., which were given an intraperitoneal inoculation with diclofenac (0 μg/kg, 0.2 μg/kg, 2.0 μg/kg, or 20.0 μg/kg) or dexamethasone (0.03 μg/kg, 0.3 μg/kg, or 3.0 μg/kg). After 12 doses, the hematological parameters and lipopolysaccharide-induced nitric oxide production by head kidney monocytic lineage were evaluated. Exposed fish also received 1 mg/kg of carrageenan intraperitoneal, and cell migration to the peritoneal cavity was evaluated after 4 h. Diclofenac and dexamethasone altered the red blood cell count, as well as hematocrit and hemoglobin levels. The total blood leukocyte count decreased in all groups. A significantly reduced carrageenan-induced leukocyte migration to the peritoneal cavity, particularly of polymorphonuclear cells, was observed at all tested doses, suggesting a possible immunosuppressive effect. The basal nitric oxide synthesis of head kidney cell cultures was reduced at the highest dose of diclofenac and was increased at the highest dose of dexamethasone. The lipopolysaccharide-stimulated nitric oxide production was reduced in all treatments, thus corroborating the immunosuppressive effect. Although some fish responses were variable for different drugs, the results suggested that trophic exposure to diclofenac and dexamethasone can lead to hematological changes and immunotoxic effects, causing negative impacts in aquatic organisms.

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Section: Experimental Designmentioning

confidence: 99%

“…This fish species has the advantage that it is easily fed under laboratory conditions and has been explored in several trophic exposure biological studies [8,9]. This fish species has the advantage that it is easily fed under laboratory conditions and has been explored in several trophic exposure biological studies [8,9].…”

Section: Introductionmentioning

confidence: 99%

Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish

Ribas

Zampronio

Assis

2015

Enviro Toxic and Chemistry

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“…Rainbow trout injected with DEX at a dose of 100 mg/kg body weight showed significant induction in 3-cyano-7-ethoxycoumarin metabolism (mainly metabolised by CYP1A enzymes), while EROD and 7-methoxyresorufin O-demethylation activities were not altered (Smith and Wilson, 2010). Similarly, injection of DEX at 0.03-3 lg/kg into male H. malabaricus did not alter EROD activity (Guiloski et al, 2015). CYP3A activity was induced in grass carp after DEX treatment (Li et al, 2008).…”

Section: Discussionmentioning

confidence: 91%

“…Dexamethasone was found to affect reproduction, growth and development in fathead minnows after chronic exposure at 500 lg L À1 (LaLone et al, 2012). It also significantly reduced resting plasma cortisol levels and induced interrenal cell atrophy following treatment with 50 mg g À1 DEX for 7 d in Chinook salmon (McQuillan et al, 2011) and was found to potentially cause oxidative stress in liver tissue at 0.3 and 3.0 lg kg À1 doses, disturbing the antioxidant system in the gonads of male Hoplias malabaricus after trophic DEX exposure at 0.03-3.0 lg kg À1 doses (Guiloski et al, 2015). Currently, there is limited information about its toxicity in the aquatic environment and the eventual effects on physiological processes in fish.…”

Section: Introductionmentioning

confidence: 93%

Does dexamethasone affect hepatic CYP450 system of fish? Semi-static in-vivo experiment on juvenile rainbow trout

et al. 2015

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“…Twice per week, the fish were fed Astyanax sp. that had been given intraperitoneal injections of either diclofenac (0, 0.2, 2.0, or 20.0 µg/kg) or dexamethasone (0, 0.03, 0.3, or 3.0 µg/kg; Guiloski et al 2015). This exposure to diclofenac decreased the glutathione- s -transferase (GST) activity in the liver, while decreased levels of testosterone were seen with both the diclofenac and dexamethasone exposures (Guiloski et al 2015).…”

Section: Toxicitymentioning

confidence: 99%

Pharmaceuticals and personal care products in waters: occurrence, toxicity, and risk

et al. 2015

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Pharmaceuticals and personal care products (PPCP) are compounds with special physical and chemical properties that address the care of animal and human health. PPCP have been detected in surface water and wastewater in the ng/L to µg/L concentration range worldwide. PPCP ecotoxicity has been studied in a variety of organisms, and multiple methods have been used to assess the risk of PPCP in the environment to ecological health. Here we review the occurrence, effects, and risk assessment of PPCP in aquatic systems, as well as the sustainability of current methods for managing PPCP contamination in aquatic systems. The major points are the following: (1) a number of PPCP present potential concerns at environmentally relevant concentrations. PPCP mixtures may produce synergistic toxicity. (2) Various methods have been used for the ecological risk assessment of PPCP in aquatic systems. There are similarities in these methods, but no consensus has emerged regarding best practices for the ecological risk assessment of these compounds. (3) Human health risk assessments of PPCP contamination in aquatic systems have generally indicated little cause for concern. However, there is a lack of information regarding whether antibiotic contamination in wastewater and aquatic systems could lead to an increase in clinically relevant antibiotic-resistant bacteria and antibiotic-resistant genes. (4) Over the next century, the combination of increasing global population size and potential droughts may result in reduced water availability, increased need for water reuse, and increasing concentrations of PPCP in wastewaters. The current wastewater treatment methods do not remove all PPCP effectively. This, coupled with the possibility that antibiotics may promote the development of antibiotic-resistant bacteria and antibiotic-resistant genes, leads to concerns about the sustainability of global water supplies.

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Effects of trophic exposure to dexamethasone and diclofenac in freshwater fish

Cited by 111 publications

References 52 publications

Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish

Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish

Does dexamethasone affect hepatic CYP450 system of fish? Semi-static in-vivo experiment on juvenile rainbow trout

Pharmaceuticals and personal care products in waters: occurrence, toxicity, and risk

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