Effects of trypsin-induced limited hydrolysis on the structural, functional, and bioactive properties of sericin

Omar, Adil; Gao, Yanhua; Wubulikasimu, Atikan; Arken, Amina; Aisa, Haji Akber

doi:10.1039/d1ra03772b

Cited by 19 publications

(8 citation statements)

References 56 publications

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“…The results showed that the MW of SH-HA using ultrasonicassisted hydrolysis were similar to the SH produced by protease from Bacillus halodurans SE5 or Alcalase and SH hydrolysed by trypsin. Furthermore, sericin extracted using Bacillus halodurans SE5 or Alcalase and hydrolysed using trypsin had MW values of 50 kDa, <15 kDa and <10 kDa, respectively, 45,46 whereas SH-HA using ultrasonic-assisted hydrolysis in the current study had protein bands in the range 20-37 kDa and at 15 kDa, indicating that the hydrolysis process with HA using ultrasonic assistance was as effective as enzymatic hydrolysis. In addition, this hydrolysis was cheaper than using enzymatic hydrolysis.…”

Section: Resultsmentioning

confidence: 58%

Factors affecting sericin hydrolysis and application of sericin hydrolysate in sericin films

2022

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Section: Resultsmentioning

confidence: 58%

Factors affecting sericin hydrolysis and application of sericin hydrolysate in sericin films

2022

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“…Reports have shown that metal‐chelating peptides are usually composed of histidine, cysteine, serine, aspartic acid, or glutamic acid (Wang et al, 2012). The remarkably higher copper chelating activity of CSH, QSH, and RSH can be ascribed to the sericin unique amino acid composition which is rich in serine (32–34%) and aspartic acid (14–16%) (Omar et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Present work aimed to prepare bioactive and functionalized sericin conjugate by the introduction of flavonoids. In our previous study, we found that sericin hydrolysates exhibit better functional and bioactive properties than sericin (Omar et al, 2021). We selected quercetin, rutin, and sericin hydrolysate and studied the physicochemical, antioxidative, and anti-inflammatory activities of flavonoid-silk sericin hydrolysate conjugates.…”

mentioning

confidence: 99%

Covalent binding of flavonoids with silk sericin hydrolysate: Anti‐inflammatory, antioxidant, and physicochemical properties of flavonoid–sericin hydrolysate conjugates

Omar

Wali

Arken

et al. 2022

Journal of Food Biochemistry

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Flavonoids, structured by the skeleton of phenyl benzopyrone, are widely occurring polyphenolic compounds in plants (Maleki et al., 2019). As common substances in the human diet, flavonoids are associated with numerous health benefits. Studies prove that a moderate habitual intake of flavonoids can reduce all-cause, cancerand cardiovascular-related mortality (Bondonno et al., 2019). The flavonoid quercetin and rutin (quercetin-3-Orhamnosylglucoside) (Figure 1) exhibit multiple bioactivities, including antioxidative, anti-inflammatory, antiviral, antidiabetic, antitumor, antibacterial, and neuroprotective activities (Gullón et al., 2017). Due to their excellent beneficial effects on human health, rutin and quercetin are popular dietary supplements (Zhang et al., 2017). The application areas of quercetin and rutin are not only restricted to the food industry, they are also used in cosmetics, for example, as an ingredient in sunscreen, and pharmaceutical industries (Berlier et al., 2013). Nevertheless, the major drawback of quercetin and rutin is their constrained bioavailability, ascribed to their poor aqueous solubility, low stability, and limited membrane permeability (Gullón et al., 2017). Studies have shown that grafting flavonoids with interactive and functional compounds to improve

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“…Sericin, a natural protein present in the degumming water from the silk industry, is recognized for its therapeutic benefits, especially in cosmeceutical applications [ 27 ]. Nevertheless, a broad peptide composition (~10–250 kDa) hinders sericin from precise therapeutic targeting and lessens its potency [ 28 , 29 , 30 ]. Optimal size distribution and specific sequences of amino acids influence the biological activity of natural proteins [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Recycled Sericin Hydrolysates Modified by Alcalase® Suppress Melanogenesis in Human Melanin-Producing Cells via Modulating MITF

Joyjamras

Netcharoensirisuk

Roytrakul

et al. 2022

IJMS

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Because available depigmenting agents exhibit short efficacy and serious side effects, sericin, a waste protein from the silk industry, was hydrolyzed using Alcalase® to evaluate its anti-melanogenic activity in human melanin-producing cells. Sericin hydrolysates consisted of sericin-related peptides in differing amounts and smaller sizes compared with unhydrolyzed sericin, as respectively demonstrated by peptidomic and SDS-PAGE analysis. The lower half-maximum inhibitory concentration (9.05 ± 0.66 mg/mL) compared with unhydrolyzed sericin indicated a potent effect of sericin hydrolysates on the diminution of melanin content in human melanoma MNT1 cells. Not only inhibiting enzymatic activity but also a downregulated expression level of tyrosinase was evident in MNT1 cells incubated with 20 mg/mL sericin hydrolysates. Quantitative RT-PCR revealed the decreased mRNA level of microphthalmia-associated transcription factor (MITF), a tyrosinase transcription factor, which correlated with the reduction of pCREB/CREB, an upstream cascade, as assessed by Western blot analysis in MNT1 cells cultured with 20 mg/mL sericin hydrolysates for 12 h. Interestingly, treatment with sericin hydrolysates for 6–24 h also upregulated pERK, a molecule that triggers MITF degradation, in human melanin-producing cells. These results warrant the recycling of wastewater from the silk industry for further development as a safe and effective treatment of hyperpigmentation disorders.

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Effects of trypsin-induced limited hydrolysis on the structural, functional, and bioactive properties of sericin

Abstract: Sericin was hydrolyzed to a limited extent using trypsin, then the physicochemical, prebiotic, and antioxidative properties of the obtained hydrolysates were investigated.

Cited by 19 publications

References 56 publications

Factors affecting sericin hydrolysis and application of sericin hydrolysate in sericin films

Factors affecting sericin hydrolysis and application of sericin hydrolysate in sericin films

Covalent binding of flavonoids with silk sericin hydrolysate: Anti‐inflammatory, antioxidant, and physicochemical properties of flavonoid–sericin hydrolysate conjugates

Recycled Sericin Hydrolysates Modified by Alcalase® Suppress Melanogenesis in Human Melanin-Producing Cells via Modulating MITF

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