Effects of tunicamycin on secretion and enzymatic activities of cellulase fromTrichoderma reesei

Merivuori, Hannele; Sands, Jeffrey A.; Montenecourt, Bland S.

doi:10.1007/bf02660119

Cited by 45 publications

(21 citation statements)

References 27 publications

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“…The glycosylation inhibitors, tunicamycin and 2-deoxy-D-glucose, have been useful in determining the importance of carbohydrate attached to cellulase and other enzymes in yeast (Sanchez et al 1982) and fungi (Hayashida and Yoshioka 1980;Merivouri et al 1985). These compounds specifically inhibited the glycosylation of asparagine in proteins (Sanchez et al 1982).…”

Section: Discussionmentioning

confidence: 99%

“…The secretion and stability of cellulases from Trichoderma has been related to the extent of enzyme glycosylation (Merivouri et al 1985;Kubicek et al 1987). Glycosylation of a variety of proteins from fungi and yeast have been reported (Merivouri et al 1985; SanOffprint requests to: R. E. Calza chez et al 1982).…”

Section: Introductionmentioning

confidence: 99%

“…Glycosylation of a variety of proteins from fungi and yeast have been reported (Merivouri et al 1985; SanOffprint requests to: R. E. Calza chez et al 1982). Protein glycosylation and its effect on cellulase secretion and activity have not been studied in ruminal fungi.…”

Section: Introductionmentioning

confidence: 99%

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Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of glycosylation inhibitors: measurement of pH and temperature optima, protease and ion sensitivities

Calza

1991

Appl Microbiol Biotechnol

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The effects of protein glycosylation inhibitors were studied in Neocallimastix frontalis EB188. Low concentrations of tunicamycin and 2-deoxy-o-glucose inhibited zoospore germination, rhizoidal elongation, carbon source utilization and the production and secretion of cellulases and proteins. The carbohydratetrimming inhibitors, deoxynojirimycin and glucono-t~-lactone, had no measurable effect on rhizoidal growth and carbon source utilization. Cellulases (intracellular or extracellular) synthesized in the presence of glycosylation inhibitors were sensitive to fl-endoglycosidase H digestion, periodate modification, certain salts, changes in incubation temperature and pH, and protease. Anthrone staining of extracellular proteins confirmed the presence of glycoproteins. In N.frontalis EB188, glycosylation of protein and cellulase occurred and was important for cellular development and the production, secretion and activity of cellulases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of glycosylation inhibitors: measurement of pH and temperature optima, protease and ion sensitivities

Calza

1991

Appl Microbiol Biotechnol

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“…Ãë³êîçèëþâàííÿ ñïðèÿº ñòàá³ë³çàö³¿ гë³êàíàç â åêñòðåìàëüíèõ óìîâàõ îòî÷óþ÷îгî ñåðåäîâèùà. Ðåêîìá³íàíòí³ êñèëàíàçè, åêñïðåñîâàí³ â Escherichia coli [39] ç àëêàë³ф³ëüíîгî, òåðìîф³ëüíîгî øòàìó Bacillus sp. [35] ïðîÿâëÿëè ìåíøó ñòàá³ëüí³ñòü ïðè âèñîê³é òåìïåðàòóð³ ³ ìåíøó çäàòí³ñòü äî çâ'ÿçóâàííÿ ç êñèëàíîì â ïîð³âíÿíí³ ç êñèëàíàçàìè âèõ³äíîгî ÊCиËÀНÀÇи ÌIÊÐÎÎÐÃÀНIÇÌIÂ øòàìó, äëÿ ÿêèõ âëàñòèâî â³äñóòí³ñòü гë³êîçèëþâàííÿ.…”

Section: продукція ксиланазиunclassified

Xylanases of Microorganisms

Varbanets¹,

Borzova²

2007

Microbiology&Biotechnology

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ÊCèËàÍàçè ÌiÊÐÎÎÐÃàÍiçÌiâÍàÿâí³ñòü âåëèêèõ ðåñóðñ³â öåëþëîçè ³ гåì³öåëþëîçè â ðîñëèíí³é á³îìàñ³ ðîáèòü âñå á³ëüø àêòóàëüíîþ ïðîáëåìó çàëó÷åííÿ ðîñëèííèõ â³äõîä³â ñ³ëüñüêîгî гîñïîäàðñòâà ³ ïðîìèñëîâîñò³ â ïðîöåñè ì³êðîáíî¿ êîíâåðñ³¿ äëÿ îòðèìàííÿ õàð÷îâîгî ³ êîðìîâîгî á³ëêà ³ ф³ç³îëîг³÷íî àêòèâíèõ ðå÷îâèí. Â³ä 20 äî 40 % â³ä ñóõî¿ âàгè ñ³ëüñüêîгîñïîäàðñüêèõ â³äõîä³â ñêëàäàº êñèëàí, ÿêèé ðàçîì ç гåì³öåëþëîçîþ óòâîðþº äðóгèé íàéá³ëüø øèðîêî ðîçïîâñþäжåíèé â á³îñфåð³ ïîë³ñàõàðèä, çäàòíèé äî â³äíîâëåííÿ. Áóëî îö³íåíî, ùî 500 ìëí òîí òàêèõ ìàòåð³àë³â ìîжóòü áóòè ùîð³÷íî äîñòóïí³ ò³ëüêè â³ä çàëèøê³â гîëîâíèõ çåðíîâèõ êóëüòóð. Òîìó ðîçâèòîê ìàëîêîøòîâíèõ òåõíîëîг³é, ÿê³ îñíîâàí³ íà гåì³öåëþëîç³, âèêëèêàº ³íòåðåñ ó äîñë³äíèê³â. йìîâ³ðíî, â íåäàëåêîìó ìàéáóòíüîìó, êñèëàí â êîìá³íàö³¿ ç öåëþëîçîþ çàáåçïå÷èòü á³ëüø³ñòü ñâ³òîâèõ ïîòðåá â ñèðîâèí³. Ìîжëèâî ïåðåäáà÷èòè, ùî â íàñòóïí³ 50 ðîê³â âóг³ëëÿ òà íåî÷èùåíà íàфòà áóäóòü çàì³íåí³ á³îìàñîþ. Â áàгàòüîõ òåõíîëîг³÷íèõ ïðîöåñàõ, ïåðø çà âñå ïðè âèðîáíèöòâ³ ïàïåðó òà öåëþëîçè, êñèëàí º íåáàжàíèì äîì³øêîì, ³ ïîâñòàº ïðîáëåìà éîгî äåгðàäàö³¿. Нåîáõ³äíî г³äðîë³çóâàòè êñèëàí òàêîж â äåÿêèõ ïðîöåñàõ õàð÷îâî¿ ³ êîðìîâî¿ ïðîìèñëîâîñò³, ïðè êîìïëåêñí³é ïåðåðîáö³ ðîñëèííèõ â³äõîä³â. Ã³äðîë³ç êñèëàíó ìîжå áóòè çä³éñíåíèé çà äîïîìîгîþ õ³ì³÷íèõ ðåàгåíò³â àáî фåðìåíòàòèâíèì øëÿõîì. Ôåðìåíòàòèâíèé г³äðîë³ç º ïåðåâàжëèâèì, îñê³ëüêè ìåíø âïëèâàº íà ñòðóêòóðó ³íøèõ âîëîêîí ³ óòâîðþºòüñÿ ìåíøå õ³ì³÷íèõ â³äõîä³â. Â õàð÷îâ³é àáî êîðìîâ³é ïðîìèñëîâîñòÿõ õ³ì³÷íèé г³äðîë³ç âçàгàë³ íåïðèéíÿòíèé. 9 Ìікробіоëоãія і біотеõноëоãія ¹ 3/2008ÊCиËÀНÀÇи ÌIÊÐÎÎÐÃÀНIÇÌIÂ Äëÿ ì³êðîáíîгî г³äðîë³çó êñèëàíó íåîáõ³äíà ä³ÿ òàêèõ фåðìåíò³â, ÿê β 1,4 êñèëàíàçè ³ β êñèëîçèäàçè. еíäî 1,4 β d êñèëàíàçà àáî 1,4 β d êñèëàí êñèëàíîг³äðîëàçà (ÊÔ 3.2.1.8) -фåðìåíò, ÿêèé çä³éñíþº íåóïîðÿäêîâàíèé г³äðîë³ç âíóòð³øíüîìîëåêóëÿðíèõ êñèëîçèäíèõ çâ'ÿçê³â êñèëàí³â àáî êñèëîîë³гîñàõàðèä³â. Âèñîêîìîëåêóëÿðí³ êñèëàíè ìàþòü ïåðåâàгó äëÿ ä³¿ фåðìåíòó â ïîð³âíÿíí³ ç îë³гîìåðíèìè ëàíöþгàìè, øâèäê³ñòü г³äðîë³çó ÿêèõ çíèжóºòüñÿ ç³ çìåíøåííÿì ñòóïåíÿ ïîë³ìåð³çàö³¿. Äðóгèé фåðìåíò, ÿêèé áåðå ó÷àñòü â ðîçùåïëåíí³ êñèëàí³â, -åêçî 1,4 β d êñèëîçèäàçà (ÊÔ 3.2.1.37) àáî β êñèëîçèäàçà, ÿêà êàòàë³çóº г³äðîë³ç îë³гîñàõàðèä³â øëÿõîì ïîñë³äîâíîгî â³äùåïëåííÿ çàëèøê³â d êñèëîçè ç íåðåäóêóþ÷îгî ê³íöÿ. Âñòàíîâëåíî, ùî àêòèâí³ñòü êñèëîçèäàçè íàéá³ëüøà ïî â³äíîøåííþ äî êñèëîá³îçè ³ çìåíøóºòüñÿ ç³ çá³ëüøåííÿì äîâжèíè ëàíöþгà ñóáñòðàòà.Äжåðåëàìè êñèëàíàç ìîжóòü áóòè áåçõðåáåòí³ òâàðèíè, âèù³ ðîñëèíè, íàñ³ííÿ, ìîðñüê³ âîäîðîñò³, àëå íàéá³ëüø òåõíîëîг³÷íèìè º ì³êðîîðгàí³çìè -гðèáè, áàêòåð³¿, äð³жäж³ [1]. Ì³êðîáí³ êñèëàíàçè çàâäÿêè ¿õ âèñîê³é ñïåöèф³÷íîñò³, ì'ÿêèì óìîâàì ðåàêö³¿, íåçíà÷íèì âèòðàòàì ñóáñòðàòà ³ óòâîðåííþ ïîá³÷íèõ ïðîäóêò³â, º íàéá³ëüø ïåðñïåêòèâíèìè êàòàë³çàòîðàìè äëÿ г³äðîë³çó êñèëàíó. Àëå âàðò³ñòü фåðìåíòàòèâíîгî г³äðîë³çó á³îìàñè º îäíèì ç фàêòîð³â, ÿêèé ë³ì³òóº åêîíîì³÷íó äîö³ëüí³ñòü ïðîöåñó. Ïðîäóêö³ÿ êñèëàíàç òèì íå ìåíø ìîжå áóòè ïîêðàùåíà çíàõîäжåííÿì á³ëüø ïîòóжíèõ øòàì³â гðèá³â àáî áàêòåð³é, àáî îäåðжàííÿì ìóòàíòíèõ øòàì³â, çäàòíèõ ïðîäóêóâà...

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“…Glycosylation of a variety of proteins from fungi and yeast have been reported (Merivouri et al 1985;Sanchez et al 1982). Protein modification and its effect on cellulase secretion and activity has not been studied in ruminal fungi.…”

Section: Introductionmentioning

confidence: 99%

Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of protein glycosylation inhibitors: measurement of protein molecular weights and isoelectric focusing values

Calza

1991

Appl Microbiol Biotechnol

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Protein and cellulase secreted in the presence of glycosylation inhibitors by Neocallimastixfrontalis EB188 were studied using gel electrophoresis. Tunicamycin and 2-deoxy-D-glucose added to established cultures inhibited the production and secretion of proteins and cellulases. Schiff reagent staining of proteins after denaturing polyacrylamide gel electrophoresis confirmed the presence of extracellular glycoproteins. Intracellular or extracellular cellulases from cultures treated with inhibitors possessed distinct isoelectric focusing values and native gel Rf values. In N. frontalis EB188, glycosylation of protein occurred and was important for the production, secretion and activity of cellulases.

show abstract

Effects of tunicamycin on secretion and enzymatic activities of cellulase fromTrichoderma reesei

Cited by 45 publications

References 27 publications

Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of glycosylation inhibitors: measurement of pH and temperature optima, protease and ion sensitivities

Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of glycosylation inhibitors: measurement of pH and temperature optima, protease and ion sensitivities

Xylanases of Microorganisms

Cellulases from Neocallimastix frontalis EB188 synthesized in the presence of protein glycosylation inhibitors: measurement of protein molecular weights and isoelectric focusing values

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