2012
DOI: 10.1007/s10295-011-1066-9
|View full text |Cite
|
Sign up to set email alerts
|

Effects of Tween 80 and pH on mycelial pellets and exopolysaccharide production in liquid culture of a medicinal fungus

Abstract: This study investigated the effects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS) production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l⁻¹ glucose and 6 g l⁻¹ peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter. The mycelial pellets became less uniform at pH (4.0-5.0) lower than the optimum (pH 6.0) or turned to filamentous form at … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
26
0

Year Published

2012
2012
2024
2024

Publication Types

Select...
7
1
1

Relationship

1
8

Authors

Journals

citations
Cited by 59 publications
(27 citation statements)
references
References 20 publications
1
26
0
Order By: Relevance
“…Tolerance was qualitatively assayed as either visible growth or no visible growth at day 25. The current assay increased the speed of the shake culture from [34] to account for the high surface tension of water at low temperatures [35] and increased the medium pH to reduce mycelial aggregation [36]. The surface tension assay was identical to the matric potential assay except that the 500 µl spore suspension contained 1% Tween 80 (average spore count  = 4.1×10 6 ±9.2×10 4 per 500 µl) which was demonstrated in a similar study [36] to reduce water surface tension.…”
Section: Methodsmentioning
confidence: 78%
“…Tolerance was qualitatively assayed as either visible growth or no visible growth at day 25. The current assay increased the speed of the shake culture from [34] to account for the high surface tension of water at low temperatures [35] and increased the medium pH to reduce mycelial aggregation [36]. The surface tension assay was identical to the matric potential assay except that the 500 µl spore suspension contained 1% Tween 80 (average spore count  = 4.1×10 6 ±9.2×10 4 per 500 µl) which was demonstrated in a similar study [36] to reduce water surface tension.…”
Section: Methodsmentioning
confidence: 78%
“…Tween 80 could increase the activity of amylase, carboxymethyl cellulose enzyme (CMCase) and xylanase of Penicillium simplicissimum isolated from compost [9]. EPS production in Cordyceps sinensis Cs-HK1 was markedly increased two-fold at 1.5% (w/v) Tween 80, which was probably related to the stimulation of EPS biosynthesis by Tween 80 [15]. It was reported that the EPS increase brought by Tween 80 was highly attributed to glucosyltransferase activity and fatty acid composition [14,16].…”
Section: Introductionmentioning
confidence: 97%
“…44 Over the past decades, emerging micropollutants such as endocrine-disrupting chemicals 45 (EDCs) including pharmaceuticals and personal care products (PPCPs) have become a matter 46 of concern in both drinking water and wastewater treatment plants (WWTPs) (Rodarte-Morales 47 et al, 2012a,b; Verlicchi et al, 2012;Li et al, 2014;Nam et al, 2015). The failure of WWTPs 48 to completely remove a wide range of PPCPs from wastewater results in the contamination of 188 (polysorbate 80) (1mL) was added to each aerated batch flask as surfactant for fungal pellet formation 189 as reported by Liu and Wu (2012). Tween ® 80 was also used for fungal extracellular enzymes protection 190 from mechanical degradation (Tien and Kirk, 1988).…”
Section: Introductionmentioning
confidence: 99%