Effects of two copper compounds on Microcystis aeruginosa cell density, membrane integrity, and microcystin release

Tsai, Kuo-Pei

doi:10.1016/j.ecoenv.2015.06.024

Cited by 47 publications

(35 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CuSO 4 5 µg/mL significantly inhibited growth of M.aeruginosa with the IE of 81.7 %. This result agreed with those of many authors [2,9] in term of the use of plant extracts and CuSO 4 as algicidesto control growth of M.aeruginosa. The pH value increased in both treatments and the control except the CuSO 4 sample during ten-day experiment (Table 3).…”

Section: Effect Of Different Plant Extracts At Concentration Of 500 µsupporting

confidence: 82%

“…The results of previous researches [9,10] showed that pH of the investigated culture medium has not only shown great effect on the growth of M.aeruginosa but also on the toxicity of the species.The decrease in the cytoplasm, chloroplast and the PS II damage at pH of 5.5 and 6.0 led to M. aeruginosa death.The possible mechanism of inhibiting the growth of M.aeruginosaby using plant extracts is due to the presence of a number of biologically active compounds, including alkaloids, allelochemicals, and phenol compounds [4,11]. Effective doses of extracts from different plant species against M.aeruginosa range from milligrams to hundreds of milligrams per liter.…”

Section: Effect Of Different Plant Extracts and Cuso 4 5 µG/ml On Ph mentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory Effect of Different Eupatoriumfortunei Turcz Extracts on the Growth of Microcystis Aeruginosa

Nga¹

2018

JST

View full text Add to dashboard Cite

Blooms of freshwater cyanobacteria have caused increasing eco-environmental problems in recent years. Recently, plant extracts have been considered as attractive alternative algicides for algal bloom treatment. The present work presented the effect of three different extracts (ethanol, water and methanol) prepared from Eupatorium fortunei Turcz at concentrations of 500 and 200 µg/mL on the growth of Microcystisaeruginosain a 10-day experiment. By using optical density method, the ethanol extract at concentration of 500 µg/mL indicated the highest toxicity with the inhibition efficiency reaching 95.5 % after 10 days which was significantly greater than those of the water, methanol extracts at the same concentration and that of CuSO 4 at 5 µg/mL (59.7 %, 66.5 % and 81,7 %, respectively). In addition, the pH of the algal suspensions was fluctuated from 6.0 to 7.0 for the control and extract-treated samples. In contrast, the pH of the CuSO 4 -treated suspension decreased to 5.0 -5.5. These results proved that the E. fortunei Turcz ethanol extract had the best properties at 500 µg/mL in comparision with other extracts.

show abstract

Section: Effect Of Different Plant Extracts At Concentration Of 500 µsupporting

confidence: 82%

Section: Effect Of Different Plant Extracts and Cuso 4 5 µG/ml On Ph mentioning

confidence: 99%

Inhibitory Effect of Different Eupatoriumfortunei Turcz Extracts on the Growth of Microcystis Aeruginosa

Nga¹

2018

JST

View full text Add to dashboard Cite

show abstract

“…According to the WHO guidance, cyanobacterial cell densities of 2 × 10 4 and 10 7 cells/mL represent guidelines for low and high health risk level in recreational waters, respectively. Tsai (2015) showed that if no treatment is implemented to reduce cell density of microcystin-producing M. aeruginosa, total microcystin concentration would increase with growing of M. aeruginosa population and potentially enhance the risks associated with exposures of microcystin (Pilotto et al, 1997). Since the minimum amount of copper required for controlling problematic algae is density-dependent, early treatments for controlling toxin-producing cyanobacteria at relatively low cell density with copper-based algaecide can not only minimize the amount of copper applied, but it would also reduce the risks related to exposures of cyanotoxins.…”

Section: Sensitivity Of Algae To Exposures Of Copper Compounds At Difmentioning

confidence: 97%

“…Furthermore, an excessive amount of copper applied for control of noxious algae in the field could also exacerbate adverse effects on nontarget species. Tsai (2015) showed that applying an excessive amount copper for controlling microcystinproducing M. aeruginosa could result in the release of relatively more microcystin compared to applying a minimum amount of copper. Microcystin released from M. aeruginosa could have allelopathic effects on aquatic phytoplankton and creatures (Pflugmacher, 2002;LeBlanc et al, 2005;Ma et al, 2015).…”

Section: Margins Of Safety For P Subcapitata At Different Cell Densimentioning

confidence: 98%

Management of Target Algae by Using Copper-Based Algaecides: Effects of Algal Cell Density and Sensitivity to Copper

Tsai

2016

Water Air Soil Pollut

Self Cite

View full text Add to dashboard Cite

Public concerns regarding the use of copperbased algaecide for controlling problematic algae may arise due to the risks it creates to non-target algae. To examine this concern, a series of comparative algal toxicity experiments were conducted to study effects of prokaryotic and eukaryotic algal cell densities on their responses to exposures of copper sulfate and copper-ethanolamine (Cu-EA). Microcystis aeruginosa and Pseudokirchneriella subcapitata were cultured separately in BG 11 medium to three initial cell densities (5 × 10 4 , 5 × 10 5 , and 5 × 10 6 cells/mL). The 96-h EC 50 values of copper sulfate for M. aeruginosa at the three cell densities were 9, 63, and 112 μg Cu/L, respectively; and were 192, 1873, and 4619 μg Cu/L for P. subcapitata. The 96-h EC 50 values of Cu-EA were 101 and 2579 μg Cu/L for M. aeruginosa and P. subcapitata at 10 6 cells/mL. The margin of safety (MOS) for P. subcapitata at 10 4 cells/mL was 1.3, 0.9, and 0.8 when M. aeruginosa cell density was 10 4 , 10 5 , and 10 6 cells/mL. This laboratory study suggests that applying copper-based algaecides to control problematic algae at a relatively low cell density would inhibit their growth with minimum impacts on non-target algae; risks to non-target algae would increase with increases of problematic algal cell density.

show abstract

“…reported that the 24-h-EC 50 of Microcystis is 0.064 mg L −1 Cu 2+ . Tsai18 reported that the minimum Cu 2+ concentration required to inhibit M. aeruginosa growth is 0.160 mg L −1 . The 96-h-EC 50 of M. aeruginosa was reported as 1.02 mg L −1 Cu 2+ by Zhang et al 19…”

mentioning

confidence: 99%

Species-dependent variation in sensitivity of Microcystis species to copper sulfate: implication in algal toxicity of copper and controls of blooms

Wei

Tan

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Copper sulfate is a frequently used reagent for Microcystis blooms control but almost all the previous works have used Microcystis aeruginosa as the target organism to determine dosages. The aim of this study was to evaluate interspecific differences in the responses of various Microcystis species to varying Cu2+ concentrations (0, 0.05, 0.10, 0.25, and 0.50 mg L−1). The half maximal effective concentration values for M. aeruginosa, M. wesenbergii, M. flos-aquae, and M. viridis were 0.16, 0.09, 0.49, and 0.45 mg L−1 Cu2+, respectively. This showed a species-dependent variation in the sensitivity of Microcystis species to copper sulfate. Malonaldehyde content did not decrease with increasing superoxide dismutase content induced by increasing Cu2+, suggesting that superoxide dismutase failed to reduce Cu2+ damage in Microcystis. Considering the risk of microcystin release when Microcystis membranes are destroyed as a result of Cu2+ treatment and the stimulation effects of a low level of Cu2+ on growth in various species, our results suggest that copper sulfate treatment for Microcystis control could be applied before midsummer when M. aeruginosa and M. viridis are not the dominant species and actual amount of Cu2+ used to control M. wesenbergii should be much greater than 0.10 mg L−1.

show abstract

Effects of two copper compounds on Microcystis aeruginosa cell density, membrane integrity, and microcystin release

Cited by 47 publications

References 67 publications

Inhibitory Effect of Different Eupatoriumfortunei Turcz Extracts on the Growth of Microcystis Aeruginosa

Inhibitory Effect of Different Eupatoriumfortunei Turcz Extracts on the Growth of Microcystis Aeruginosa

Management of Target Algae by Using Copper-Based Algaecides: Effects of Algal Cell Density and Sensitivity to Copper

Species-dependent variation in sensitivity of Microcystis species to copper sulfate: implication in algal toxicity of copper and controls of blooms

Contact Info

Product

Resources

About