Effects of two-dimensional materials on human mesenchymal stem cell behaviors

Suhito, Intan Rosalina; Han, Yoojoong; Kim, Da-Seul; Son, Hyungbin; Kim, Tae-Hyung

doi:10.1016/j.bbrc.2017.08.149

Cited by 32 publications

(33 citation statements)

References 35 publications

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“…No loss of cell viability was observed for any of the materials up to concentrations of 100 μg mL −1 . Furthermore, cells seeded onto the 2D material thin films showed strong interactions with the substrates, similar to what was observed in the previous study by Suhito et al 152 This favorable cell attachment and high biocompatibility have prompted further studies on the biological interactions of nanostructured films of 2D materials with different cell types, such as differentiation of stem cells. 154 Similar biocompatibility has also been reported for a number of polymer composites containing 2D materials.…”

Section: D Materials Hazard Assessmentsupporting

confidence: 83%

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Assessing and Mitigating the Hazard Potential of Two-Dimensional Materials

Guiney¹,

Wang

Xia

et al. 2018

ACS Nano

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The family of two-dimensional (2D) materials is comprised of a continually expanding palette of unique compositions and properties with potential applications in electronics, optoelectronics, energy capture and storage, catalysis, and nanomedicine. To accelerate the implementation of 2D materials in widely disseminated technologies, human health and environmental implications need to be addressed. While extensive research has focused on assessing the toxicity and environmental fate of graphene and related carbon nanomaterials, the potential hazards of other 2D materials have only recently begun to be explored. Herein, the toxicity and environmental fate of post-carbon 2D materials, such as transition metal dichalcogenides, hexagonal boron nitride, and black phosphorus, are reviewed as a function of their preparation methods and surface functionalization. Specifically, we delineate how the hazard potential of 2D materials is directly related to structural parameters and physicochemical properties, and how experimental design is critical to the accurate elucidation of the underlying toxicological mechanisms. Finally, a multidisciplinary approach for streamlining the hazard assessment of emerging 2D materials is outlined, thereby providing a pathway for accelerating their safe use in a range of technologically relevant contexts.

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Section: D Materials Hazard Assessmentsupporting

confidence: 83%

“…(B-D) Cell viability of the modified substrates after 2 days and 7 days as determined by growth rate using a CCK-8 assay. Figure adapted from reference 152 …”

Section: Figurementioning

confidence: 99%

Assessing and Mitigating the Hazard Potential of Two-Dimensional Materials

Guiney¹,

Wang

Xia

et al. 2018

ACS Nano

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“…The immunoblot assay and immunofluorescence analysis indicated the Akt-mTOR-p70S6K signaling pathway played a critical role in the induction of cellular proliferation and differentiation by single-layer MoS 2 in HELFs (Zou et al, 2017). Based on the observation of cell morphology, human mesenchymal stem cells on MoS 2 film exhibited more active filopodial interaction with substrate compared to the control group, indicating that MoS 2 highly enhanced cell adhesion and spreading (Suhito et al, 2017). In human hepatoma HepG2 cells, MoS 2 nanosheets induced mitochondrial depolarization and elevated reactive oxygen species level (Liu et al, 2017).…”

Section: Biosafety Evaluation Of 2d Tmdcsmentioning

confidence: 97%

“…However, the interaction between 2D TMDCs and cells certainly will disturb cell homeostasis. Further biosafety evaluation indicated that 2D TMDCs affected cell behaviors, such as cell proliferation, differentiation (Zou et al, 2017), cell adhesion, spreading (Suhito et al, 2017), oxidative stress (Yang et al, 2014), cell metabolism (Yu Y. et al, 2017), and cell autophagy . Singlelayer MoS 2 nanosheets promoted proliferation and accelerated myogenic differentiation in human embryonic lung fibroblasts (HELFs).…”

Section: Biosafety Evaluation Of 2d Tmdcsmentioning

confidence: 99%

Two-Dimensional Transition Metal Dichalcogenides: Synthesis, Biomedical Applications and Biosafety Evaluation

Zhou

Sun

Bai

2020

Front. Bioeng. Biotechnol.

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Recently, two-dimensional transition metal dichalcogenides (2D TMDCs) have drawn certain attentions in many fields. The unique and diversified electronic structure and ultrathin sheet structure of 2D TMDCs offer opportunities for moving ahead of other 2D nanomaterials such as graphene and expanding the wide application of inorganic 2D nanomaterials in many fields. For a better understanding of 2D TMDCs, one needs to know methods for their synthesis and modification, as well as their potential applications and possible biological toxicity. Herein, we summarized the recent research progress of 2D TMDCs with particular focus on their biomedical applications and potential health risks. Firstly, two kinds of synthesis methods of 2D TMDCs, top-down and bottom-up, and methods for their surface functionalization are reviewed. Secondly, the applications of 2D TMDCs in the field of biomedicine, including drug loading, photothermal therapy, biological imaging and biosensor were summarized. After that, we presented the existing researches on biosafety evaluation of 2D TMDCs. At last, we discussed major research gap in current researches and challenges and coping strategies in future studies.

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“…Various nanobiomaterials (e.g., nanoparticles, porous polymers, and nanopatterning) have proven to be favorable in increasing cell spreading and focal adhesion through supportive topographical cues [21][22][23][24][25][26][27]. In this regard, the enhancement of the specific differentiation of various types of stem cells could also be influenced by the advantageous properties of biocompatible nanomaterials [28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Enhancing Neurogenesis of Neural Stem Cells Using Homogeneous Nanohole Pattern-Modified Conductive Platform

Cho

Kim

Suhito

et al. 2019

IJMS

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Biocompatible platforms, wherein cells attach and grow, are important for controlling cytoskeletal dynamics and steering stem cell functions, including differentiation. Among various components, membrane integrins play a key role in focal adhesion of cells (18–20 nm in size) and are, thus, highly sensitive to the nanotopographical features of underlying substrates. Hence, it is necessary to develop a platform/technique that can provide high flexibility in controlling nanostructure sizes. We report a platform modified with homogeneous nanohole patterns, effective in guiding neurogenesis of mouse neural stem cells (mNSCs). Sizes of nanoholes were easily generated and varied using laser interference lithography (LIL), by changing the incident angles of light interference on substrates. Among three different nanohole patterns fabricated on conductive transparent electrodes, 500 nm-sized nanoholes showed the best performance for cell adhesion and spreading, based on F-actin and lamellipodia/filopodia expression. Enhanced biocompatibility and cell adhesion of these nanohole patterns ultimately resulted in the enhanced neurogenesis of mNSCs, based on the mRNAs expression level of the mNSCs marker and several neuronal markers. Therefore, platforms modified with homogeneous nanohole patterns fabricated by LIL are promising for the precise tuning of nanostructures in tissue culture platforms and useful for controlling various differentiation lineages of stem cells.

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Effects of two-dimensional materials on human mesenchymal stem cell behaviors

Cited by 32 publications

References 35 publications

Assessing and Mitigating the Hazard Potential of Two-Dimensional Materials

Assessing and Mitigating the Hazard Potential of Two-Dimensional Materials

Two-Dimensional Transition Metal Dichalcogenides: Synthesis, Biomedical Applications and Biosafety Evaluation

Enhancing Neurogenesis of Neural Stem Cells Using Homogeneous Nanohole Pattern-Modified Conductive Platform

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