Effects of two in-office bleaching agents with different pH values on enamel surface structure and color: An in situ vs. in vitro study

Sa, Yue; Chen, Dongping; Liu, Yi; Wen, W; Xu, Meng; Jiang, Tao; Wang, Yining

doi:10.1016/j.jdent.2012.02.010

Cited by 85 publications

(76 citation statements)

References 34 publications

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“…We showed that application of this agent to enamel subsurface lesions did not aggravate the lesions 37) . Also, remineralization solution or natural human saliva could eliminate the demineralization effect caused by in-office bleaching agents 38,39) . Furthermore, high-concentration hydrogen peroxide generated preferential mineralization in the deeper region and subsequently overall enhanced mineralization 40) .…”

Section: Discussionmentioning

confidence: 99%

Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions —Experimental study with bovine lesion model systems—

et al. 2014

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Salivary macromolecules infiltrate white and brown spot enamel lesions and adsorb onto hydroxyapatite. Calcium-binding salivary proteins such as statherin hinder remineralization of these lesions. We assessed whether bleaching agents can remove salivary components that have infiltrated and bound to experimental subsurface lesions in bovine enamel prepared by immersing specimens in acid and then human saliva. Transversal microradiography showed that such demineralized lesions mimicked incipient carious lesions. Bound proteins to the experimental and untreated control specimens were eluted in a stepwise manner with phosphatebuffered saline, 0.4 M phosphate buffer, and 1 M HCl. SDS-PAGE of dialyzed extracts showed that specific salivary proteins bound to the lesions, while virtually no protein bands were detected if the specimens were bleached. Western blotting showed that even statherin, which was more firmly bound than other proteins, was removed. In-office bleaching agent may be useful in treating enamel lesions for removing proteins bound to these lesions.

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Section: Discussionmentioning

confidence: 99%

Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions —Experimental study with bovine lesion model systems—

et al. 2014

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“…Several studies suggest that saliva may influence the loss of mineral components caused by tooth whitening because salivary flow stimulation increases the presence of components, such as carbonic acid, hydrogen carbonate, hydrogen phosphate, and calcium fluoride, which are associated with increased buffering capacity and maintenance of the balance between demineralization and remineralization. 51,52 In situ studies about…”

Section: -44mentioning

confidence: 99%

In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching

Gomes¹,

Alexandrino²,

Alencar³

et al. 2017

The Journal of Contemporary Dental Practice

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Aim: Evaluate in situ the effect of nanohydroxyapatite paste (nano-HAP) before bleaching with hydrogen peroxide 35% (HP35%) by ion chromatography (IC) Knoop hardness number (KHN) and tristimulus colorimetry (TC). Materials and methods:A total of 60 fragments were obtained from third molars included (3 mm × 3 mm × 3 mm) and the specimens were divided into three groups (n = 20): Gas chromatography (CG) (negative control group) = no bleaching; HP35% (positive control group) = HP35% whitening (whiteness HP35%); nano-HAP = application for 10 minutes before bleaching treatment + HP35%. The specimens were fixed to the volunteers' molars. The KHN and TC were measured before and after bleaching. For IC, the dentin layer was removed, leaving the enamel that was crushed, and autoclaved for chemical quantification (calcium, fluorine, and phosphorus). The results of KHN and TC were analyzed statistically by analysis of variance (ANOVA) followed by Tukey test (p < 0.05). Results:The HP35% group showed reduction of the Ca, F, and P ions. The initial and final KHN mean of the CG and nano-HAP did not differ statistically; however, the group of HP35% did differ statistically. The mean ΔE of the HP35% and nano-HAP groups did not differ statistically from each other. However, they differed from the CG. Conclusion:The nano-HAP paste preserved the KHN, promoted the lower loss of Ca and P ions and an increase of F ions when compared with the CG, but did not influence the effectiveness of the bleaching treatment.Clinical significance: Nano-HA is a biomaterial that has shown positive results in the prevention of deleterious effects on the enamel by the action of the office bleaching treatment.

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“…Whitening toothpastes, which are used every day, should also have a neutral pH; it is reported that some toothpastes had a highly acidic pH as low as 3.67±0.06 (29). Acidic and neutral in-office bleaching agents have the same whitening efficiency in situ and in vitro (30). In this study DASH gel had pH values of 4.64 ± 0.08 while BOOST gel was close to neutral with pH 7.25 ± 0.01.…”

Section: šAntak I Surmentioning

confidence: 50%

“…Zubne paste za izbjeljivanje koje se rabe svaki dan, trebale bi imati neutralni pH, no istraživanje je pokazalo da neke imaju vrlo nizak pH -3,67 ± 0,06 (29). Kiseli ili neutralni gelovi za izbjeljivanje imaju isti učinak izbjeljivanja in situ i in vitro (30). U ovoj studiji bila je izmjerena pH vrijednost za DASH od 4,64 ± 0,08 a za BO-OST je pH bio blizu neutralnoga -7,25 ± 0,01.…”

Section: šAntak I Surunclassified