Effects of two proteinase inhibitors on the digestive enzymes and survival of honey bees (Apis mellifera)

Burgess, Elisabeth P. J.; Malone, Louise A.; Christeller, John T.

doi:10.1016/0022-1910(96)00045-5

Cited by 90 publications

(74 citation statements)

References 28 publications

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“…A reduction in some of the proteolytic activity in the midgut of the honey bee, after administering a 1% dose of SBTI in the diet, was also noted by Sagili et al (2005). A confirmation of these results can be found in the earlier studies by Burgess, Malone, and Christeller (1996). These researchers observed that a 1% dose of SBTI in the diet reduced protein biosynthesis in the HPGs of bees and considerably decreased the level of three endopeptidases, such as chymotrypsin, elastase, and trypsin.…”

Section: Discussionsupporting

confidence: 60%

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Wilde

Frączek

Siuda

et al. 2016

Journal of Apicultural Research

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The effect of sublethal doses of imidacloprid on protein content and activity of proteases on honey bees was analyzed. The study was conducted in three experimental groups: colonies from groups BE-5 and BE-200 were contaminated with 5 and 200 ppb of imidacloprid, respectively, via their food supply (syrup and pollen), while group BE was used as control (untreated). Bee samples were collected 3 and 10 weeks after feeding started. Protein concentration in bee tissue extracts was analyzed with reference: (a) to the dose of imidacloprid; and (b) duration of exposure to the chemical. The average quantity of protein content was significantly higher at the 3-week interval than in the 10-week interval and the bees from control colonies (BE) had significantly higher protein contents than contaminated bees (BE-5 and BE-200), even 3 weeks after feeding with imidacloprid started. Similarly, the activity of proteolytic enzymes (proteases) was found to be dependent on the dose of imidacloprid used, compared to bees from control colonies showing significantly higher activity.Influencia de las dosis subletales de Imidacloprid en el contenido de proteína y la actividad proteolítica en las abejas melíferas (Apis mellifera L.)Se ha analizado el efecto de las dosis subletales de imidacloprid en el contenido de proteínas y la actividad de las proteasas en las abejas melíferas. El estudio se realizó en tres grupos experimentales: las colonias de los grupos de BE-5 y BE-200 se contaminaron con 5 y 200 ppb de imidacloprid, respectivamente, a través del alimento suministrado (jarabe y polen), mientras que el grupo SER se utilizó como control (sin tratar). Se recogieron muestras de abejas en las semanas 3 y 10 después del comienzo de la alimentació n. La concentració n de proteína en extractos de tejido de abejas se analizó en relació n con: a) la dosis de imidacloprid; y b) la duració n de la exposició n a la sustancia química. La cantidad promedio del contenido de proteína fue significativamente mayor en el intervalo de 3 semanas que en el intervalo de 10 semanas y las abejas de las colonias control (BE) tuvieron un contenido de proteína significativamente más alto que las abejas contaminadas (BE-5 y BE-200), incluso 3 semanas después de que comenzara la alimentació n con imidacloprid. Del mismo modo se encontró que la actividad de las enzimas proteolíticas (proteasas) depende de la dosis de imidacloprid usada, en comparació n con las abejas de colonias de control que muestran una actividad significativamente más alta.

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Section: Discussionsupporting

confidence: 60%

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Wilde

Frączek

Siuda

et al. 2016

Journal of Apicultural Research

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“…When plants grow with their full complement of natural ecological interactions, these costs are likely to be balanced by the fitness benefits resulting from the defensive utility of TPI expression (24). However, ecological costs might also be incurred that result from the complicated interactions with other species (2,16), such as the decrease the attractiveness of the pollinators (2,41). Because TPI expression frequently slows the grow rate of insect herbivores by making their digestive processes less efficient (40,42), the fitness benefits of TPI expression may result from extending the period during which larvae can be successfully attacked by natural enemies.…”

Section: Discussionmentioning

confidence: 99%

Constitutive and inducible trypsin proteinase inhibitor production incurs large fitness costs in Nicotiana attenuata

Zavala

Patankar

Gase

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

197

198

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Plant trypsin proteinase inhibitors (TPIs) are potent herbivore-and jasmonate (JA)-induced defenses, but support for the commonly invoked explanation for their inducible expression, namely their associated fitness costs, has been elusive. To determine whether the expression of TPIs incurs fitness costs, we expressed 175 bp of the seven-domain pi from Nicotiana attenuata in an antisense orientation in a TPI-producing genotype (WT) of N. attenuata to reduce TPI expression. Moreover, we expressed the full-length seven-domain pi in a sense orientation under control of a constitutive promoter to restore TPI activity in a natural genotype unable to produce TPIs because of a mutation in its endogenous pi gene. Lifetime reproductive output was determined from high and low TPI-producing plants of the same genetic background with and without JA elicitation and grown in the same pot to simulate natural competitive and nutrient regimes. Transformants with either low or no TPI activity grew faster and taller, flowered earlier, and produced more seed capsules (25-53%) than did neighboring TPI-producing genotypes, and JA elicitation increased TPI production and decreased seed capsule production further. Growth under high light levels only marginally reduced these fitness costs. Results were similar regardless of whether TPI activity was suppressed or restored by transformation: the larger the difference in TPI activity between neighbors, the larger the difference in seed capsule production (R 2 ‫؍‬ 0.57). TPI production is costly for a plant's components of fitness when grown under realistic competitive regimes and is consistent with the hypothesis that inducibility evolved as a cost-saving mechanism.

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“…proteinase inhibitors (PIs) (Brødsgaard et al, 2003;Burgess et al, 1996;Sagili et al, 2005), Bacillus thuringiensis (Bt) toxins (Arpaia, 1996;Malone et al, 2004) and biotinbinding proteins (Malone et al, 2002). Some have been conducted using transgenic plant material on adults (Babendreier et al, 2005;Liu et al, 2005), but few have considered the effects of transgenic plants on honey bee larval stages (Hanley et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

Lehrman

2007

Environ. Biosafety Res.

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The European honey bee (Apis mellifera) is important both for pollination and for honey production. Pollen is the major protein source for bees, which exposes them directly to changes in pollen quality e.g. through genetic engineering. In order to create a worst case scenario regarding pea lectin (PSL) expressed transgenically in oilseed rape anthers and pollen, the maximum amount of dried pollen that could be mixed in an artificial diet without negatively affecting larval performance (1.5% w/w) was fed to bee larvae. Pollen from two transgenic plant lines expressing PSL up to 1.2% of total soluble protein and pollen from one non-transgenic line was added to the same diet and used as a pollen control. When these three pollen diets and the control diet (without added pollen) were compared, no negative effect from the pollen of the transgenic plants could be detected on larval mortality, weight, or development time. An increased weight and a reduced developmental time were recorded for larvae on all diets containing pollen when compared to the diet without pollen.

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Effects of two proteinase inhibitors on the digestive enzymes and survival of honey bees (Apis mellifera)

Cited by 90 publications

References 28 publications

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Constitutive and inducible trypsin proteinase inhibitor production incurs large fitness costs in Nicotiana attenuata

Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

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