Silva, LM, Neiva, HP, Marques, MC, Izquierdo, M, and Marinho, DA. Short post-warm-up transition times are required for optimized explosive performance in team sports. J Strength Cond Res 36(4): 1134–1140, 2022—This study aimed to compare the effect of 3 different post-warm-up transition times (3, 10, and 17 minutes) on team sports performance. A randomized crossover research design was used. Fourteen university male basketball players completed the same 10-minute warm-up followed by a transition time of 3, 10, or 17 minutes until the performance assessment. In the control condition, no warm-up was performed. The performance was measured using the repeated sprint and jump ability test. Performance variables (time, jump height, and peak power) and physiological variables (lactate and tympanic temperature) were analyzed. Moderate effects were found between conditions for the best first 12.5-m sprint (F = 1.91, p = 0.17, normalηp2 = 0.13), with faster times after 3-minute transition (control: 2.51 ± 0.12 vs. 3 minutes: 2.41 ± 0.15, p = 0.02, effect size [ES] = 0.74). Jump heights were higher after transitions of 3 minutes (38.55 ± 5.07 cm, p < 0.01, ES = 0.58), 10 minutes (37.69 ± 4.92 cm, p < 0.01, ES = 0.40), and 17 minutes (37.87 ± 5.33 cm, p < 0.01, ES = 0.42) compared with the control condition (35.84 ± 4.18 cm). However, no significant differences were found between resting conditions. The warm-up caused a moderate increase in lactate and temperature compared with no activity (F = 11.90, p < 0.01, normalηp2 = 0.48; F = 2.56, p = 0.07, normalηp2 = 0.16, respectively), but changes from preperformance to postperformance evaluation showed no differences between experimental conditions. The results showed that the warm-up maximized the performance of explosive efforts. However, no significant differences were found between transition times. Despite a trend toward optimized explosive performances after a short post-warm-up transition time (3 minutes), further research is needed.