Effects of type and state of co-culture cells on in-vitro development of porcine oocytes matured and fertilized in vitro

Qian, Yun; Shi, Wei; Ding, Jia Tong; Liu, Jia Yin; Sha, Jia Hao; Fan, Bi Qin

doi:10.1007/s10815-005-5145-6

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…In vivo , fertilization has been shown to occur during this period in equine or porcine species [ 63 , 64 ]. Moreover, porcine oviduct cells have a better competence to support porcine embryo development when they are collected at ovulation or corpus luteum stages than at other ovarian cyclic stages [ 65 ]. Thus, we used oviduct cells collected during the in vivo fertilization period to keep the favourable environment of fertilization.…”

Section: Discussionmentioning

confidence: 99%

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Mugnier

Kervella

Douet

et al. 2009

Reprod Biol Endocrinol

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BackgroundOviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse.Methods & resultsIn the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we co-incubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes.In order to identify the proteins responsible for the positive effect of OEC, we first searched for the presence of the genes encoding oviductin, osteopontin and atrial natriuretic peptide A (ANP A) in the equine genome. We showed that the genes coding for osteopontin and ANP A are present. But the one for oviductin either has become a pseudogene during evolution of horse genome or has been not well annotated in horse genome sequence. We then showed that osteopontin and ANP A proteins are present in the equine oviduct using a surface plasmon resonance biosensor, and we analyzed their expression during oestrus cycle by Western blot. Finally, we co-incubated equine gametes with or without purified osteopontin or synthesized ANP A. No significant effect of osteopontin or ANP A was observed, though osteopontin slightly increased the IVF rates.ConclusionOur study shows a beneficial effect of homologous and heterologous oviduct cells on equine IVF rates, though the rates remain low. Furthers studies are necessary to identify the proteins involved. We showed that the surface plasmon resonance technique is efficient and powerful to analyze molecular interactions during fertilization.

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Section: Discussionmentioning

confidence: 99%

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Mugnier

Kervella

Douet

et al. 2009

Reprod Biol Endocrinol

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show abstract

“…Even if this argument is correct, it should be noted that in suboptimal conditions, which are most often present in ART laboratories, coculture systems might eliminate the harmful effects of environmental factors on embryo development. Many studies have reported the beneficial effects of co-cultures on embryo development in vitro in humans [1,32,33]; domestic animals [34,35] and rodents [19,26,27]. However, it is not fully elucidated why co-culture of embryos with somatic cells improves embryo development.…”

Section: Discussionmentioning

confidence: 99%

Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

Nematollahi‐Mahani

Pahang

Moshkdanian

et al. 2009

J Assist Reprod Genet

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“…An alternative method to improve the in vitro development of oocytes and embryos is coculturing with somatic cells (17)(18)(19)(20). However, the use of a feeder layer for improving the IVM of isolated follicles has received less attention (21).…”

mentioning

confidence: 99%

The effect of leukemia inhibitory factor and coculture on the in vitro maturation and ultrastructure of vitrified and nonvitrified isolated mouse preantral follicles

Haidari

Salehnia

Valojerdi

2008

Fertility and Sterility

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Effects of type and state of co-culture cells on in-vitro development of porcine oocytes matured and fertilized in vitro

Abstract: As compared with GCs and pOECs at follicular stage, the pOECs at ovulation and CL stages had a better competence to support porcine embryo development under in vitro conditions.

Cited by 12 publications

References 21 publications

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

The effect of leukemia inhibitory factor and coculture on the in vitro maturation and ultrastructure of vitrified and nonvitrified isolated mouse preantral follicles

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