“… and ) are generated from the decomposition of water vapor trapped in the collapsing bubbles due to the extremely high temperature and pressure formed in collapsing bubbles [ [25] , [26] ], and their recombination or reaction with other gaseous species in the bubble leads to the formation of active species such as , and [ [18] , [32] ]. It has been reported that more hydroxyl radicals were generated at high frequencies than at low frequencies (1120 kHz > 740 kHz > 20 kHz) [ 29 ]. Besides, the amount of free radicals increases with the sonication time [ [25] , [29] , [33] ].…”