Effects of Ultraviolet‐B and PUVA on Ornithine Decarboxylase Activity, DNA Synthesis, and Protein Kinase C Activity in Mouse Skin

Mizuno, Nobuyuki; Kono, Toru; Taniguchi, Shoji; Fukuda, Michio; Maekawa, Naoki; Hisa, Tomoyuki; Otani, Shuzo; Hamada, Toshio

doi:10.1111/j.1346-8138.1993.tb03834.x

Cited by 3 publications

(2 citation statements)

References 30 publications

(16 reference statements)

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“…The partial inhibition by bisindolyl maleimide and the observation that TPA enhances reporter activity in MM96L-gal but not in HeLa-gal suggest a role for PKCmediated induction of MT (17,26,27). Solar radiation enhances expression of PKC (28,29). Further, hexamethylene bisacetamide, a less active analogue of ABHA, causes translocation of PKC (30).…”

Section: Discussionmentioning

confidence: 99%

Biphasic Response of the Metallothionein Promoter to Ultraviolet Radiation in Human Melanoma Cells

Hansen

Ablett

Green

et al. 1997

Photochem & Photobiology

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Because metallothionein (MT) is elevated and may be protective in UV-irradiated skin, we have studied the effects of UV and other agents on MT transcription using the sheep MT 1A promoter, linked to the beta-galactosidase gene and stably transfected into human cell lines. beta-galactosidase reporter activity was inducible by adding Zn2+ ions to the medium (100 microM for 2-4 h). Two differentiating agents, butyric acid and azelaic bishydroxamic acid (ABHA), significantly increased the response to Zn2+ in a melanoma cell line (MM96L-gal). UVB (280-315 nm) had two distinct, time-dependent effects. During the first 4 h after irradiation, high doses of UVB inhibited induction by Zn2+, an effect that was made more acute by simultaneous exposure to the differentiating agents. These changes in reporter activity were not due to alterations in Zn2+ transport into the cell. The UVB-depressed MT response subsequently recovered and by 24 h was double the control, yet remained sensitive to ABHA. Reporter activity in transfected HeLa cells differed from that in MM96L, being depressed 4 and 24 h after UVB and insensitive to ABHA at both times. Galactosidase reporter activity driven by non-MT promoters was not affected by these treatments. Dependence of MT transcriptional activity on UV-related DNA damage could be inferred because equitoxic UVC (254 nm) affected the response to Zn2+ in a similar fashion, whereas UVA, cisplatin and a methylating agent had no effect. The MT response was partly dependent on the PKC signal transduction pathway because it was inhibited by phorbol ester in HeLa, and by bisindolyl maleimide in HeLa and MM96L. The biphasic MT transcriptional response may model a signal transduction pathway that gives an early, depressed response to acute UV damage, with exacerbation by concurrent differentiation stimuli, but switches to a positive, cell-specific and potentially protective response at later times.

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Section: Discussionmentioning

confidence: 99%

Biphasic Response of the Metallothionein Promoter to Ultraviolet Radiation in Human Melanoma Cells

Hansen

Ablett

Green

et al. 1997

Photochem & Photobiology

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“…regarded as a stress protein [15] and is induced by UV irradiation [16][17][18][19] and by exposure to reactive oxygen species [20,21]. Since the products of ODC activity are polyamines, it is conceivable that the production of polyamines upon ODC induction serves to protect cells against DNA lesions and free radical damage.…”

Section: Introductionmentioning

confidence: 99%

Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation

Nilsson

Gritli-Linde

Heby

2000

Biochemical Journal

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Hemizygous gyro male (Gy/Y) mice are a model for X-linked hypophosphataemic rickets. As in humans, the disease is caused by deletions in the Phex gene, a phosphate-regulating gene having homologies with endopeptidases on the X chromosome. Some phenotypic abnormalities in Gy/Y mice have recently been attributed to the fact that the Gy deletion also includes the neighbouring spermine synthase gene, resulting in spermine deficiency. Spermine and its precursors spermidine and putrescine are essential for cell growth and differentiation. As a novel method for studying the function of spermine, we established primary cultures of skin fibroblasts from hemizygous Gy/Y mice. The Gy/Y cells contained no detectable spermine. In view of the fact that spermine is a free-radical scavenger in vitro, we were surprised to find that Gy/Y cells were more resistant to oxidative stress than their normal (X/Y) counterparts. However, our finding that spermidine accumulates markedly in the spermine-deficient Gy/Y cells can probably explain this increased resistance. It is the first indication that spermidine can serve as a free-radical scavenger in vivo and not only in vitro. When subjecting the Gy/Y cells to UV-C irradiation we made another interesting finding: the mutant cells were more sensitive than the normal X/Y cells. This finding indicates that spermine, probably because of its high-affinity binding to DNA, is important in protection against chromatin damage.

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Vieillissement cutané: aspects anatomophysiologiques

Berbis¹

2006

EMC - Dermatologie

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Effects of Ultraviolet‐B and PUVA on Ornithine Decarboxylase Activity, DNA Synthesis, and Protein Kinase C Activity in Mouse Skin

Cited by 3 publications

References 30 publications

Biphasic Response of the Metallothionein Promoter to Ultraviolet Radiation in Human Melanoma Cells

Biphasic Response of the Metallothionein Promoter to Ultraviolet Radiation in Human Melanoma Cells

Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation

Vieillissement cutané: aspects anatomophysiologiques

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