Effects of Underwater Lipus on the Morphohistology of the CF-1 Mice Growth Plate in Late Lactation Stage

Abstract

Morphol., 35(4):1351Morphol., 35(4): -1358Morphol., 35(4): , 2017. SUMMARY:Ultrasound is one of the most used tools in physiotherapy, its LIPUS modality allows to treat musculoskeletal injuries, but like the conventional ultrasound it is contraindicated its application in areas close to growth cartilage by a possible closure of this one. In the literature there is no conclusive evidence of this contraindication, so the objective of the present study is to evaluate the effects of doses equivalent to underwater LIPUS on the histomorphometry of the proximal tibial epiphysis growth plate in an animal model. LIPUS was applied in underwater mode in the right pelvic limb to 8 mice in p 15 for 4 days, 2 times a day. On completion of 8 sessions the histology of the stimulated limb growth plate was sacrificed and the histology. When evaluating the histomorphometry of the plaque, a significant increase in the thickness and area of the proliferative zone stimulated with LIPUS (p = 0.048) was observed. In the hypertrophic area were observed no significant differences in thickness or areas, but in the hypertrophic area there were significant changes in the histological organization, observing a significant increase of the entire columns in the stimulated plaque (p = 0.04). LIPUS in underwater modality modifies the histomorphometry of the proximal epiphyseal PC of the tibia of rodents in the late postnatal stage, promoting proliferation and columnar organization in the proliferative and hypertrophic zone, respectively.

Potential Application of Low-Intensity Pulsed Ultrasound in Delaying Aging for Mice

“…Materials with high air or interface reflectivity are not used because they cause significant ultrasonic energy loss. When water is the coupling medium of sound propagation, except that the sound attenuation of water to sound waves is small, because of the fast heat transfer rate in water, it can quickly take away heat and reduce the temperature on the surface of tissue, so as to ensure the safety of treatment [33]. Therefore, we further proposed a novel underwater LIPUS exposure setup (shown in Fig.…”

Effects of Therapeutic Ultrasound on Growth Plates: A Systematic Review

“…After multiple rounds of exclusion, 8 studies were included in the final analysis (Figure 1). [15][16][17][18][19][20][21][22]…”

“…Four studies used continuous ultrasound, 15,16,18,22 and 3 used pulsed ultrasound. 17,19,21 In the study by Wiltink et al, 20 both protocols were used. All studies used frequencies common in the day-to-day practice of therapeutic ultrasound in physical therapy, which ranges between 0.8 and 1.5 MHz.…”

“…All studies used frequencies common in the day-to-day practice of therapeutic ultrasound in physical therapy, which ranges between 0.8 and 1.5 MHz. A wide range of output power was used, from 0.03 W/cm 2 SATA (spatial average temporal average) in pulsed mode, 19,21 to 7 W/cm 2 SATA in continuous application. 22 The duration of treatment ranged from 3 to 20 minutes for each session in different studies.…”

“…Others treated participants for 8 to 28 sessions in total. Six studies were performed on growth plates around the stifle joint (equivalent to the knee in humans), 15,16,18,19,21,22 1 on the distal radius and ulna, 17 and 1 on metatarsal growth (Table 2). 20…”