Effects of Unilateral Ovariectomy on Growth of Oocytes in the Ovaries of Adult Toads (Bufo bufo bufo L.)

Jørgensen, C. Barker; E, Billeter; Poulsen, Kirsten M.

doi:10.1095/biolreprod20.2.346

Biology of Reproduction

1979

DOI: 10.1095/biolreprod20.2.346

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Effects of Unilateral Ovariectomy on Growth of Oocytes in the Ovaries of Adult Toads (Bufo bufo bufo L.)

C. Barker Jørgensen¹,

Billeter E²,

Kirsten M. Poulsen³

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Cited by 10 publications

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Growth, differentiation, and function of the testes in the toad Bufo bufo bufo (L.), with special reference to regulatory capacities: Effects of unilateral castration, hypophysectomy, and excision of bidder's organs

Jøorgensen

1982

J. Exp. Zool.

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Function and compensatory growth of the testis have been studied in male toads of varying ages, ranging from postmetamorphosis to adults. All experiments lasted 2 months, starting about September 1. Spermatogenesis was normal in sexually immature, 1-year-old toads. The fastest growing young toads developed secondary sex characteristics, the thumbpads, during the experiment. The data suggest that size is more influential than age in determining the onset of sexual maturation. But body size is not the only factor which influences sexual maturation. The growth response of the remaining testis to unilateral castration decreased with increasing age and size of the toads. Two months after operation the remaining testis had reached the size of two testes in the young toads, whereas the testis showed no significant compensatory growth in the adult-sized toads, whether sexually immature or mature. Hypophysectomy reduced body growth in young toads and prevented a testicular growth response to unilateral castration. Young toads, but no adults, with excised Bidder's organs had larger testes than the control toads. In hypophysectomized, young toads spermatogenesis was still active 2 months after the operation.

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Growth, differentiation, and function of the testes in the toad Bufo bufo bufo (L.), with special reference to regulatory capacities: Effects of unilateral castration, hypophysectomy, and excision of bidder's organs

Jøorgensen

1982

J. Exp. Zool.

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Mechanisms regulating the ovarian cycle in the frog Rana cyanophlyctis: Effect of unilateral ovariectomy, season, feeding, human chorionic gonadotrophin, and estradiol‐17β

Saidapur

Prasadmurthy

1987

J. Exp. Zool.

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Effects of 30-day unilateral ovariectomy (ULO) on compensatory ovarian hypertrophy (COH) was studied in adult frog Rana cyanophlyctis in relation to reproductive phase/season, feeding, and treatment with human chorionic gonadotrophin (HCG) or estradiol-17 beta. Compensatory growth of the remaining ovary was assessed in terms of weight as well as changes in the dynamics of the pool sizes of different oocytes viz., first growth phase (FGP), medium-sized second growth phase (MSGP), large-sized second growth phase (LSGP), and atretic oocytes. The frogs were fed with live guppies 6 days a week. The effect of underfeeding on COH was studied in the frogs fed once a week. The ovaries removed at operation and those of the sham-operated frogs were used for comparison (controls). COH occurred in both pre- and postbreeding phases (February and November, respectively). In both instances follicular atresia was greatly reduced. In February, COH was due to recruitment of both MSGP and LSGP oocytes. The number of these oocytes increased significantly over controls, and the ovarian weight nearly doubled. However, in November COH occurred because of an increase in FGP oocytes and therefore total oocytes, but there were no changes in the ovarian weights. Administration of 20 IU HCG (6 days a week) had no influence on the basic pattern of COH-response exhibited by the remaining ovary in relation to reproductive phase/season, but it increased the recruitment of oocytes, i.e., FGP oocytes in November and SGP oocytes in February. Follicular atresia was drastically reduced with HCG. Underfeeding or treatment with estradiol-17 beta abolished the COH in both February and November.(ABSTRACT TRUNCATED AT 250 WORDS)

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Unilateral ovariectomy increases egg size and reduces follicular atresia in the semelparous coho salmon, Oncorhynchus kisutch

et al. 2008

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Unilateral ovariectomy (ULO, removal of one ovary) is a powerful technique for studying aspects of reproductive physiology, including follicular recruitment and growth. To examine effects of ULO for the first time in a semelparous species, coho salmon (Oncorhynchus kisutch) were unilaterally ovariectomized during mid-vitellogenesis approximately 3 months before spawning. At termination of the study (79 days post-surgery), single ovaries of ULO fish were gravimetrically equivalent to paired ovaries of sham surgery, control fish. There was no evidence of recruitment of new vitellogenic follicles. Instead, the dramatic increase in ovary mass was attributable to hypertrophy of existing vitellogenic follicles (33% increase in volume) and increased fecundity achieved through a greater than two-fold reduction in follicular atresia. The composition of whole ovaries on a dry weight basis from ULO fish was greater in protein, but lower in lipid than that of control fish. Expressing the data on a per follicle basis, however, showed that follicles of ULO fish contained more protein, ash, water, and lipid. The results indicate that ULO of coho salmon induces compensatory hypertrophy of existing vitellogenic follicles, while maximizing fecundity through reduction of atresia. Thus, 3 months before spawning, coho salmon exhibit the ability to adjust final egg size and number when faced with significant depletion of ovarian follicles. This in vivo system provides a platform for further study of physiological mechanisms regulating follicular growth and atresia, and the trade-off between egg size and egg number.

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Effects of Unilateral Ovariectomy on Growth of Oocytes in the Ovaries of Adult Toads (Bufo bufo bufo L.)

Cited by 10 publications

References 4 publications

Growth, differentiation, and function of the testes in the toad Bufo bufo bufo (L.), with special reference to regulatory capacities: Effects of unilateral castration, hypophysectomy, and excision of bidder's organs

Growth, differentiation, and function of the testes in the toad Bufo bufo bufo (L.), with special reference to regulatory capacities: Effects of unilateral castration, hypophysectomy, and excision of bidder's organs

Mechanisms regulating the ovarian cycle in the frog Rana cyanophlyctis: Effect of unilateral ovariectomy, season, feeding, human chorionic gonadotrophin, and estradiol‐17β

Unilateral ovariectomy increases egg size and reduces follicular atresia in the semelparous coho salmon, Oncorhynchus kisutch

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