Effects of unprocessed versus vinegar-processed Schisandra chinensis on the activity and mRNA expression of CYP1A2, CYP2E1 and CYP3A4 enzymes in rats

Su, Tao; Mao, Chunqin; Yin, Fan; Yu, Zhi‐Ling; Lin, Ying-Hsuan; Song, Yu; Lu, Tulin

doi:10.1016/j.jep.2013.01.028

Cited by 37 publications

(21 citation statements)

References 20 publications

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“…Such induction of CYP2E1 was attenuated when rats supplemented with triterpenoid from Schisandra chinensis following alcohol exposure. Interestingly, Su et al (2013) reported that CYP2E1 enzyme activity was inhibited after treatment with multiple doses of vinegar-processed Schisandra chinensis [36]. Conversely, another group found that multiple dosing of the extracts from Schisandra chinensis significantly induces the activity of CYP2E1 [37].…”

Section: Discussionmentioning

confidence: 99%

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

Zhu

et al. 2014

Cell Biochem Biophys

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Alcohol-induced oxidative stress plays a crucial role in the pathological development of alcoholic liver disease. The aim of this study was to investigate the effects of triterpenoid from Schisandra chinensis on oxidative stress in alcohol-induced liver injury in rats. We found that the administration of triterpenoid attenuated alcohol-induced oxidative stress in multiple organs including liver. Moreover, the impaired liver function and histological changes resulted from alcohol consumption was improved by triterpenoid treatment. Finally, we found that pretreatment with triterpenoid from Schisandra chinensis to alcohol-fed rats increased the expression level of haem oxygenase-1 (HO-1) while inhibited the induction of cytochrome P-450 2E1 (CYP2E1) in liver microsomes. Further assays revealed that the microsomal activity of HO-1 was accordingly induced whereas CYP2E1 was suppressed in rats received triterpenoid intervention. Our findings suggest that triterpenoid from Schisandra chinensis may protect against alcohol-induced liver injury through ameliorating oxidative stress in rats.

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Section: Discussionmentioning

confidence: 99%

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

Zhu

et al. 2014

Cell Biochem Biophys

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“…Therefore, we speculated that flavonoids might be active compounds that can reduce the impact of liver-damaging substances by inhibiting the expression of CYP2E1, which might be one of the hepatoprotective mechanisms of flavonoids. Also, an investigation of the effects of flavonoids on CYP2E1 expression is necessary since some daily foods (purple potato, purple sweet potato, broccoli, bitter gourd, garlic, and tomato), beverages (coffee and cacao polyphenol), and drugs ( Schisandra chinensis , danshen, Gelsemium elegans , isoniazid, diallyl sulfide, sulforaphane, and tamoxifen) are metabolized by CYP2E1 (Vang et al, 1991; Gu et al, 1992; Wargovich, 2006; Wang et al, 2010, 2015; Konstandi et al, 2013; Su et al, 2013; Ahmad et al, 2014; Lu et al, 2014; Zhou et al, 2014, 2015; Shayakhmetova et al, 2015; Suzuki et al, 2015; Jiang et al, 2016a,b). …”

Section: Introductionmentioning

confidence: 99%

Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines

Jiang

et al. 2016

Front. Pharmacol.

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Cytochrome P450 (CYP) 2E1 is an important enzyme involved in the metabolism of many endogenous and exogenous compounds. It is essential to evaluate the expression of CYP2E1 in the studies of drug–drug interactions and the screening of drugs, natural products, and foodstuffs. The present work is a feasibility study on the development of immunoassays using a specific and sensitive chicken-sourced anti-CYP2E1 IgY antibody. Cloning, expression, and purification of a recombinant CYP2E1 (mice origin) protein were carried out. Anti-CYP2E1 IgY antibodies were generated by immunizing white Leghorn chickens with purified recombinant CYP2E1 protein and were purified by immune affinity chromatography. The IgY titer attained a peak level (≥1:128,000) after the fifth booster injection. For evaluation of the expression of CYP2E1 in different herbal treatment samples, the mice were treated by oral gavage for 3 days with alcohol (50% 15 mL/kg), acetaminophen (APAP, 300 mg/kg), Cornus officinalis extract (100 mg/kg), Alhagi-honey extract (100 mg/kg), Apocynum venetum extract (100 mg/kg), hyperoside (50 mg/kg), isoquercetin (50 mg/kg), 4-hydroxyphenylacetic acid (50 mg/kg), 3-hydroxyphenylacetic acid (50 mg/kg), and 3,4-hydroxyphenylacetic acid (50 mg/kg). The expression of CYP2E1 was determined by Western blot analysis, immunohistochemistry, ELISA, and immunomagnetic beads (IMBs) using anti-CYP2E1 IgY in liver tissue. The results showed that C. officinalis extract, Alhagi-honey extract, A. venetum extract, hyperoside, isoquercetin, and their xenobiotics 4-hydroxyphenylacetic acid, 3-hydroxyphenylacetic acid, and 3,4-hydroxyphenylacetic acid significantly decreased CYP2E1 levels. Alcohol and APAP treatments significantly increased CYP2E1 levels as analyzed with Western blot analysis, immunohistochemistry, and ELISA. The IMB method is suitable for large-scale screening, and it is a rapid screening (20 min) that uses a portable magnet and has no professional requirements for the operator, which makes it useful for on-the-spot analysis. Considering these results, the anti-CYP2E1 IgY could be applied as a novel research tool in screening for the CYP2E1 inhibitor/enhancer.

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“…SC and VSC are widely used in TCM practice. Previous research has suggested that vinegar processing enhances the increase or decrease in CYP450 enzyme (CYP1A2, CYP2E1 and CYP3A4) levels elicited by SC and VSC [26]. No new lignan compounds were found after vinegar processing, but the amounts of lignan components were changed [37].…”

Section: Discussionmentioning

confidence: 99%

“…We have researched on the effects of SC and VSC on the activity of CYP1A2, CYP2E1 and CYP3A4 enzymes in rats in previous studies [26]. The 10 most significant drug metabolizing CYPs in the human liver include CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2E1, CYP2D6 and CYP3A4/5.…”

Section: Introductionmentioning

confidence: 99%

Effects of Unprocessed Versus Vinegar-Processed Schisandra chinensis on the Activity of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 Enzymes in Rats

Su¹,

Wang²,

Li³

et al. 2017

J Anal Bioanal Tech

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Schisandra chinensis (SC) is a well-known traditional Chinese herbal medicine that has been used in clinical practices for thousands of years. However, the differences between the effects of unprocessed and vinegarprocessed Schisandra chinensis (VSC) on cytochrome P450 (CYP450) activities are poorly understood. To evaluate the differences between processed and unprocessed SC on the metabolism of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 substrates in rats using a cocktail method based on a developed and validated high performance liquid chromatography-mass spectrometry (HPLC-MS) method. Six probe substrates (coumarin (CYP2A6), bupropion (CYP2B6), paclitaxel (CYP2C8), tolbutamide (CY2C9), omeprazole (CYP2C19) and metoprolol (CYP2D6)) were delivered simultaneously into rats treated with single or multiple doses of processed or crude SC extract. The plasma concentrations of the six probes were profiled by HPLC-MS, and their corresponding pharmacokinetic parameters were calculated. Treatment with single or multiple doses of either extract of SC or VSC induced CYP2A6, CYP2B6 and CYP2C9 enzyme activity and inhibited CYP2D6, CYP2C19 and CYP2C8 enzyme activity in rats. Furthermore, the inhibitory or induced effect of multiple doses of SC was more potent after vinegar processing than without vinegar processing. CYP2A6, 2B6 and 2C9 enzyme activity were induced significantly after treatment with multiple doses but not after a single dose. CYP2C19 enzyme activity were inhibited significantly after treatment with multiple doses but not after a single dose. These results provide useful scientific data for the safe clinical application of either extract of SC in combination with other drugs, which should lack the side effects induced by other herb-drug interactions.

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Effects of unprocessed versus vinegar-processed Schisandra chinensis on the activity and mRNA expression of CYP1A2, CYP2E1 and CYP3A4 enzymes in rats

Cited by 37 publications

References 20 publications

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines

Effects of Unprocessed Versus Vinegar-Processed Schisandra chinensis on the Activity of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 Enzymes in Rats

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