Effects of uraemia and dialysis modality on polymorphonuclear cell apoptosis and function

Sardenberg, Camila; Suassuna, Paulo Giovanni de Albuquerque; Andreoli, Maria Cláudia Cruz; Watanabe, Renato; Dalboni, Maria Aparecida; Manfredi, Sílvia Regina; Santos, Oscar Pavão dos; Kallas, Esper G.; Draibe, Sérgio Antônio; Cendoroglo, Miguel

doi:10.1093/ndt/gfi095

Cited by 60 publications

(53 citation statements)

References 19 publications

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“…Chronic kidney disease is a state that induces apoptosis, but this is normalized with continuous and high-flux hemodialysis modalities (D'Intini et al 2004;Bordoni et al 2006). This is in accordance with studies showing that dialysis membrane characteristics affect leukocyte cell apoptosis (Martin-Malo et al 2000;Sela et al 2005;Sardenberg et al 2006). The degree of spontaneous apoptosis of leukocytes is higher when bioincompatible membranes are used for hemodialysis, than when biocompatible membranes are used (Martin-Malo et al 2000).…”

Section: Patients On Hemodialysissupporting

confidence: 52%

Leukocyte Function in High-Flux Hemodialysis

Olsson¹

2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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Section: Patients On Hemodialysissupporting

confidence: 52%

Leukocyte Function in High-Flux Hemodialysis

Olsson¹

2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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“…Instead a large number of studies using experimental models and human subjects have shown that uremia is associated with apoptosis in a wide range of cells and tissues such as skeletal muscle [19,20] , myocardium [21] , platelets [22,23] , monocytes [24] , neutro phils [25] , lymphocytes [26] , leukocytes [27] and vascular endothelial cells [28] . The kidney has also been shown as a target for apoptosis in uremia with both podocytes [29] and proximal tubular cells identified as having increased apoptotic cell death [30] .…”

Section: Uremia Induced Apoptosismentioning

confidence: 99%

Aging and uremia: Is there cellular and molecular crossover?

White

Yaqoob

Harwood

2015

WJN

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immune systems. However, whilst much has been documented about the shared physical characteristics of aging and uremia, the molecular and cellular similarities between the two have received less attention. In order to bridge this perceived gap we have reviewed published research concerning the common molecular processes seen in aging subjects and CKD patients, with specific attention to altered proteostasis, mitochondrial dysfunction, post-translational protein modification, and senescence and telomere attrition. We have also sought to illustrate how the cell death and survival pathways apoptosis, necroptosis and autophagy are closely interrelated, and how an understanding of these overlapping pathways is helpful in order to appreciate the shared molecular basis behind the pathophysiology of aging and uremia. This analysis revealed many common molecular characteristics and showed similar patterns of cellular dysfunction. We conclude that the accelerated aging seen in patients with CKD is underpinned at the molecular level, and that a greater understanding of these molecular processes might eventually lead to new much needed therapeutic strategies of benefit to patients with renal disease. AbstractMany observers have noted that the morphological changes that occur in chronic kidney disease (CKD) patients resemble those seen in the geriatric population, with strikingly similar morbidity and mortality profiles and rates of frailty in the two groups, and shared characteristics at a pathophysiological level especially in respect to the changes seen in their vascular and REVIEW

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“…PMNLs from patients with chronic kidney disease (CKD) are characterized by an increased basal activity and a diminished reactivity upon stimulation leading to an increased incidence of morbidity and mortality (1)(2)(3). The former feature contributes to the inflammatory state described in uremic patients and as a consequence to an increased risk for cardiovascular diseases.…”

Section: Resistin Inhibits Essential Functions Of Polymorphonuclear Lmentioning

confidence: 99%

Resistin Inhibits Essential Functions of Polymorphonuclear Leukocytes

Cohen

Ilić

Raupachova

et al. 2008

The Journal of Immunology

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The serum levels of resistin, a 12-kDa protein primarily expressed in inflammatory cells in humans, are increased in patients with chronic kidney disease and in those with diabetes mellitus. Both groups of patients have an increased risk of infections mainly as a result of disturbed polymorphonuclear leukocyte (PMNL) functions. Therefore, we investigated the influence of resistin on human PMNLs. Serum resistin concentrations were determined with a sandwich enzyme immunoassay. Using PMNLs from healthy subjects, chemotaxis was tested by the under-agarose method. Flow cytometric assays to measure oxidative burst and phagocytosis were conducted in whole blood. The uptake of deoxyglucose was determined as measure of the PMNL activation state. The activity of intracellular kinases was assessed by Western blotting and by in vitro kinase assays. Resistin inhibited PMNL chemotaxis and decreased the oxidative burst stimulated by Escherichia coli and by PMA, but did not influence PMNL phagocytosis of opsonized E. coli and PMNL glucose uptake. The inhibition of PMNLs by resistin was observed at concentrations found in serum samples of uremic patients, but not in concentrations measured in healthy subjects. Experiments with specific signal transduction inhibitors and measurements of intracellular kinases suggest that PI3K is a major target of resistin. In conclusion, resistin interferes with the chemotactic movement and the stimulation of the oxidative burst of PMNL, and therefore may contribute to the disturbed immune response in patients with increased resistin serum levels such as uremic and diabetic subjects.

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Effects of uraemia and dialysis modality on polymorphonuclear cell apoptosis and function

Abstract: Our results suggest that both uraemia and treatment modality may interfere with PMN apoptosis and function. Dialysis appears to normalize the increased PMN apoptosis rates observed in CKD patients.

Cited by 60 publications

References 19 publications

Leukocyte Function in High-Flux Hemodialysis

Leukocyte Function in High-Flux Hemodialysis

Aging and uremia: Is there cellular and molecular crossover?

Resistin Inhibits Essential Functions of Polymorphonuclear Leukocytes

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