Effects of uridine, isomatitol and 4-thiouridine on in vitro cell adhesion and in vivo effects of 4-thiouridine in a lung inflammation model

Uppugunduri, Srinivas; Gautam, Chamilly

doi:10.1016/j.intimp.2004.04.016

Cited by 12 publications

(17 citation statements)

References 24 publications

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“…This was also seen in our previous study where a uridine analogue, 4-thiouridine, was found to reduce the TNF release with nearly 80% (see Ref. [3]), but this time we found a large variation in the TNF levels within the SDX group. None of the samples showed such high levels as those seen in the previous study.…”

Section: Discussionsupporting

confidence: 88%

“…Our previous results [3] with uridine in an in vitro adhesion assay showed that exogenous uridine affected TNF-induced static adhesion between granulocytes and cultured human umbilical cord endothelial cells (HUVEC). The in vitro model was designed primarily to mimic the up-regulation of adhesion molecules on the endothelium and subsequent granulocyte adhesionboth pivotal steps in leukocyte migration.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown [3] that uridine seems to affect in vitro adhesion between granulocytes and cultured endothelial cells without a clear dose-response relationship. Leukocyte influx plays an important role in the inflammatory response.…”

Section: Introductionmentioning

confidence: 98%

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Anti-inflammatory effects of exogenous uridine in an animal model of lung inflammation

Evaldsson

Rydén

Uppugunduri

2007

International Immunopharmacology

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory effects of exogenous uridine in an animal model of lung inflammation

Evaldsson

Rydén

Uppugunduri

2007

International Immunopharmacology

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“…Isomaltitol was therefore included in a screening model of static adhesion between isolated granulocytes and cultivated human umbilical cord endothelial vein cells. Results from this previous study [2] showed that the presence of isomaltitol at concentrations between 1 m M to 1 n M reduced the number of adhering neutrophils to TNF-stimulated endothelial cells. It was speculated that isomaltitol might interact with adhesion molecules.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously described isomaltitol in an in vitro model of static adhesion between polymorphonuclear cells and TNF-stimulated endothelial cells [2]. In that setting, isomaltitol reduced neutrophil adhesion by roughly 40% at concentrations between 1 m M and 1 n M .…”

Section: Introductionmentioning

confidence: 99%

Isomaltitol Exacerbates Neutrophilia but Reduces Eosinophilia: New Insights into the Sephadex Model of Lung Inflammation

Evaldsson¹,

Rydén

Uppugunduri

2010

Int Arch Allergy Immunol

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Background: We have previously examined isomaltitol in an in vitro static adhesion assay and were interested in investigating whether the potentially anti-inflammatory effects observed there could be relevant in vivo. The Sephadex-induced lung inflammation model was considered a suitablemodel due to the significant changes in global inflammatory endpoints seen upon provocation with Sephadex. Methods: Male Sprague-Dawley rats were instilled intratracheally with Sephadex (5 mg/ml), vehicle (0.9% NaCl), isomaltitol (50 mg/ml) or a combination of isomaltitol and Sephadex. After 24 h, the lungs were weighed to measure edema and preserved for histology. Bronchoalveolar lavage fluid was used for analysis of tumor necrosis factor, cysteinyl leukotrienes, and differential and total leukocyte counts. Results: Differential counts showed that isomaltitol increased the neutrophil component while decreasing the eosinophilia, thus asserting a modulatory role on the usually eosinophil-dominated Sephadex-induced cell profile. Isomaltitol alone also increased edema and cysteinyl leukotrienes, and generally aggravated total inflammation in combination with Sephadex. The mechanisms were not investigated in this study, but effects could relate to a combination of isomaltitol’s osmotic and structure-specific properties. Conclusion: Our results show that isomaltitol can modulate the inflammatory response induced by Sephadex instillation in addition to having proinflammatory effects on its own, and may therefore provide new insights into the mechanisms of this widely used animal model. Sugar alcohols similar to isomaltitol have already been used to aid mucus clearance in cystic fibrosis patients, and it is possible that isomaltitol could also be used for this purpose.

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Modulatory effects of inosine, guanosine and uridine on lipopolysaccharide-evoked increase in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats

Kovács¹,

Kékesi²,

Juhász³

et al. 2015

Brain Research Bulletin

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Effects of uridine, isomatitol and 4-thiouridine on in vitro cell adhesion and in vivo effects of 4-thiouridine in a lung inflammation model

Cited by 12 publications

References 24 publications

Anti-inflammatory effects of exogenous uridine in an animal model of lung inflammation

Anti-inflammatory effects of exogenous uridine in an animal model of lung inflammation

Isomaltitol Exacerbates Neutrophilia but Reduces Eosinophilia: New Insights into the Sephadex Model of Lung Inflammation

Modulatory effects of inosine, guanosine and uridine on lipopolysaccharide-evoked increase in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats

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