Effects of UV/H2O2 degradation on Moringa oleifera Lam. leaves polysaccharides: Composition, in vitro fermentation and prebiotic properties on gut microorganisms

Yang, Min; Tao, Liang; Wang, Zi-Lin; Li, Ling-Fei; Zhao, Cun-Chao; Shi, Chong-Ying; Sheng, Jun; Tian, Yang

doi:10.1016/j.fochx.2024.101272

Cited by 4 publications

(1 citation statement)

References 61 publications

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“…At 120-180 min, the pH values tended to stabilize, indicating that the content of hydroxyl radicals (•OH) decreased and most of the main sites attacked by free radicals in polysaccharides had been broken after degradation for 120 min [13]. The above results were consistent with those of the study by Yang et al [14].…”

Section: Ph Values and Molecular Weightssupporting

confidence: 91%

Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan

He,

Zhu,

Deng

et al. 2024

Marine Drugs

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The applications of fucoidan in the food industry were limited due to its high molecular weight and low solubility. Moderate degradation was required to depolymerize fucoidan. A few studies have reported that fucoidan has potential antibacterial activity, but its antibacterial mechanism needs further investigation. In this study, the degraded fucoidans were obtained after ultraviolet/hydrogen peroxide treatment (UV/H2O2) at different times. Their physicochemical properties and antibacterial activities against Staphylococcus aureus and Escherichia coli were investigated. The results showed that the average molecular weights of degraded fucoidans were significantly decreased (up to 22.04 times). They were mainly composed of fucose, galactose, and some glucuronic acid. Fucoidan degraded for 90 min (DFuc-90) showed the strongest antibacterial activities against Staphylococcus aureus and Escherichia coli, with inhibition zones of 27.70 + 0.84 mm and 9.25 + 0.61 mm, respectively. The minimum inhibitory concentrations (MIC) were 8 mg/mL and 4 mg/mL, respectively. DFuc-90 could inhibit the bacteria by damaging the cell wall, accumulating intracellular reactive oxygen species, reducing adenosine triphosphate synthesis, and inhibiting bacterial metabolic activity. Therefore, UV/H2O2 treatment could effectively degrade fucoidan and enhance its antibacterial activity.

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Section: Ph Values and Molecular Weightssupporting

confidence: 91%

Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan

He,

Zhu,

Deng

et al. 2024

Marine Drugs

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Ultra-high pressure assisted extraction of water-soluble polysaccharides from Moringa oleifera seed husks and their antioxidant activities

Wang,

Liu,

Wang

et al. 2024

International Journal of Food Properties

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Calcium Transport Activity of UV/H2O2-Degraded Fucoidans and Their Structural Characterization

Zhu,

Wang,

You

et al. 2024

Marine Drugs

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Calcium-chelated polysaccharides have been increasingly considered as promising calcium supplements. In this study, degraded fucoidans (DFs) with different molecular weights (Mws) were prepared after UV/H2O2 treatment; their calcium-chelating capacities and intestinal absorption properties were also investigated. The results showed that the calcium-chelating capacities of DFs were improved with a decrease in Mw. This was mainly ascribed to the increased carboxyl content, which was caused by free-radical-mediated degradation. Meanwhile, the conformation of DF changed from a rod-like chain to a shorter and softer chain. The thermodynamic analysis demonstrated that DF binding to calcium was spontaneously driven by electrostatic interactions. Additionally, DF-Ca chelates with lower Mw showed favorable transport properties across a Caco-2 cell monolayer and could effectively accelerate the calcium influx through intestinal enterocytes. Furthermore, these chelates also exhibited a protective effect on the epithelial barrier by alleviating damage to tight junction proteins. These findings provide an effective free-radical-related approach for the development of polysaccharide-based calcium supplements with improved intestinal calcium transport ability.

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Effects of UV/H2O2 degradation on Moringa oleifera Lam. leaves polysaccharides: Composition, in vitro fermentation and prebiotic properties on gut microorganisms

Cited by 4 publications

References 61 publications

Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan

Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan

Ultra-high pressure assisted extraction of water-soluble polysaccharides from Moringa oleifera seed husks and their antioxidant activities

Calcium Transport Activity of UV/H2O2-Degraded Fucoidans and Their Structural Characterization

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