Effects of validamycin in controlling Fusarium head blight caused by Fusarium graminearum: Inhibition of DON biosynthesis and induction of host resistance

Li, Jing; Duan, Yabing; Bian, Chuanhong; Pan, Xiayan; Yao, Chengjie; Wang, Jianxin; Zhou, Mingguo

doi:10.1016/j.pestbp.2018.11.012

Cited by 54 publications

(58 citation statements)

References 51 publications

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“…After the incubation of an additive for 6 days, the culture liquid was collected and the mycelia were dried and weighed. DON production in the culture liquid was measured using the DON ELISA Kit (Wise, Zhenjiang, China) according to a previous study [7]. The DON ELISA Kit uses an indirect competitive ELISA method to detect the DON content in strains and cereals.…”

Section: Methodsmentioning

confidence: 99%

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Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat

Zhou

et al. 2019

Toxins

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Deoxynivalenol (DON) is a class of mycotoxin produced in cereal crops infected with Fusarium graminearum species complex (FGSC). In China, FGSC mainly includes Fusarium asiaticum and F. graminearum. DON belongs to the trichothecenes and poses a serious threat to the safety and health of humans and animals. Succinate dehydrogenase inhibitors (SDHIs) are a class of fungicides that act on succinate dehydrogenase and inhibit the respiration of pathogenic fungi. In this study, the fungicidal activities of five SDHIs, including fluopyram, flutolanil, boscalid, benzovindiflupyr, and fluxapyroxad, against FGSC were determined based on mycelial growth and spore germination inhibition methods. The five SDHIs exhibited better inhibitory activities in spore germination than mycelial growth. Fluopyram exhibited a higher inhibitory effect in mycelial growth and spore germination in comparison to the other four SDHIs. In addition, the biological characteristics of F. asiaticum as affected by the five SDHIs were determined. We found that these five SDHIs decreased DON, pyruvic acid and acetyl-CoA production, isocitrate dehydrogenase mitochondrial (ICDHm) and SDH activities, and NADH and ATP content of F. asiaticum but increased the citric acid content. In addition, TRI5 gene expression was inhibited, and the formation of toxisomes was disrupted by the five SDHIs, further confirming that SDHIs can decrease DON biosynthesis of F. asiaticum. Thus, we concluded that SDHIs may decrease DON biosynthesis of F. asiaticum by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle. Overall, the findings from the study will provide important references for managing Fusarium head blight (FHB) caused by FGSC and reducing DON contamination in F. asiaticum-infected wheat grains.

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Section: Methodsmentioning

confidence: 99%

“…DON has been demonstrated to be the most common contamination associated with FGSC-infected wheat grains and can cause hematic and anorexic syndromes as well as neurotoxic and immunotoxic effects in mammals. Additionally, DON has also been reported as an important virulence factor of FGSC [6,7].…”

Section: Introductionmentioning

confidence: 99%

Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat

Zhou

et al. 2019

Toxins

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“…The inhibition of the deoxynivalenol (DON) production of F. graminearum strain PH−1 was tested according to the method of Li et al [25] with slight modifications. In brief, a 1 mL of spore suspension (1 × 10 6 spores/mL) of F. graminearum strain PH−1 was inoculated with 100 mL of GYEP that contained the indicated concentrations of AgNPs.…”

Section: Effect Of Agnps On Deoxynivalenol Productionmentioning

confidence: 99%

Green-Synthesization of Silver Nanoparticles Using Endophytic Bacteria Isolated from Garlic and Its Antifungal Activity against Wheat Fusarium Head Blight Pathogen Fusarium graminearum

et al. 2020

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Nanoparticles are expected to play a vital role in the management of future plant diseases, and they are expected to provide an environmentally friendly alternative to traditional synthetic fungicides. In the present study, silver nanoparticles (AgNPs) were green synthesized through the mediation by using the endophytic bacterium Pseudomonas poae strain CO, which was isolated from garlic plants (Allium sativum). Following a confirmation analysis that used UV–Vis, we examined the in vitro antifungal activity of the biosynthesized AgNPs with the size of 19.8–44.9 nm, which showed strong inhibition in the mycelium growth, spore germination, the length of the germ tubes, and the mycotoxin production of the wheat Fusarium head blight pathogen Fusarium graminearum. Furthermore, the microscopic examination showed that the morphological of mycelia had deformities and collapsed when treated with AgNPs, causing DNA and proteins to leak outside cells. The biosynthesized AgNPs with strong antifungal activity were further characterized based on analyses of X-ray diffraction, transmission electron microscopy, scanning electron microscopy, EDS profiles, and Fourier transform infrared spectroscopy. Overall, the results from this study clearly indicate that the biosynthesized AgNPs may have a great potential in protecting wheat from fungal infection.

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“…According to a new study, validamycin, a type of fungicide for the control of crop diseases caused by Rhizoctonia species, had a remarkable effect on reducing DON synthesis by F. graminearum [153,154]. Further research on the molecular mechanism revealed that validamycin decreased glucose production by targeting trehalase and blocking the glycolytic pathway, thereby reducing pyruvate and DON production [153]. This finding provides us with important reference values and guidance in the screening of appropriate fungicides against harmful metabolites, as no fungicidal activity of validamycin against F. graminearum was discovered in vitro.…”

Section: Fusarium Toxin Managementmentioning

confidence: 99%

Fusarium Toxins in Chinese Wheat since the 1980s

Qiu

Shi

2019

Toxins

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Wheat Fusarium head blight (FHB), caused by Fusarium species, is a widespread and destructive fungal disease. In addition to the substantial yield and revenue losses, diseased grains are often contaminated with Fusarium mycotoxins, making them unsuitable for human consumption or use as animal feed. As a vital food and feed ingredient in China, the quality and safety of wheat and its products have gained growing attention from consumers, producers, scientists, and policymakers. This review supplies detailed data about the occurrence of Fusarium toxins and related intoxications from the 1980s to the present. Despite the serious situation of toxin contamination in wheat, the concentration of toxins in flour is usually lower than that in raw materials, and food-poisoning incidents have been considerably reduced. Much work has been conducted on every phase of toxin production and wheat circulation by scientific researchers. Regulations for maximum contamination limits have been established in recent years and play a substantial role in ensuring the stability of the national economy and people's livelihoods.

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Effects of validamycin in controlling Fusarium head blight caused by Fusarium graminearum: Inhibition of DON biosynthesis and induction of host resistance

Cited by 54 publications

References 51 publications

Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat

Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat

Green-Synthesization of Silver Nanoparticles Using Endophytic Bacteria Isolated from Garlic and Its Antifungal Activity against Wheat Fusarium Head Blight Pathogen Fusarium graminearum

Fusarium Toxins in Chinese Wheat since the 1980s

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