Effects of vanadate, N₂ and light on the membrane potential of motor cells and the lateral leaflet movements of Desmodium motorium

Abstract

The lateral leaflets of Desmodium motorium (Houtt.) Merr. exhibit ultradian up‐ and down movements, which are paralleled by oscillations of the membrane potential of motor cells in the pulvinus. By different treatments we have tested the hypothesis that both that both oscillation‐types are causally related. The reactions of the leaflet movement and the membrane potential were evaluated by the following approaches. (1) Application of vanadate. an inhibitor of the proton pump in the plasmalemma. and N2 suppressed leaflet movements and finally arrested the leaflet in the lower position. Before the oscillations damped out, a strong lengthening in period was found. This indicates that the pump is part of the ultradian clock. A period lenthening and a final suppression of the rhythm by vanadate was also seen in the extracellular electric potential of the pulvinus. Intracellular recordings in situ showed that vanadate application depolarized the motor cells. (2) Light of high fluence rates diminished the amplitude of the oscillations of the membrane potential of single motor cells and shortened the period. The same effects were observed when monitoring the lateral leaflet movement. The leaflet always moved towards the direction of the light. whether it was applied from the abaxial or from the adaxial part of the pulvinus. (3) When light was applied to the pulvinus of lateral leaflets. which had spontancously stopped moving in an upper position. oscillations were induced transiently. This effect was also found for the membrane potential of motor cells in the pulvinus. ‐ Our results thus provide further evidence that the membrane potential controls the volume state of the motor cells in the pulvinus of lateral leaflets of Desmodium motorium.

Nyctinastic movement in legumes: Developmental mechanisms, factors and biological significance

“…The role of K + channels in leaf movement is discussed in detail later. More direct evidence came from experiments with Codariocalyx motorius , where white light applied to the adaxial side of the pulvinus caused upward lateral leaf movement, whereas light applied to the abaxial side caused downward movement (Antkowiak et al, 1992). In Phaseolus , the volume of pulvinus motor cells decreases by 50% upon light stimulation, whereas the surface area is reduced by 33% (Koller & Zamski, 2002).…”

Insights Into the Inside – A Quantitative Histological Study of the Explosively Moving Style in Marantaceae

“…(Oxalidaceae) (Umrath, 1928), Desmodium gyrans (L.f.) DC. (Fabaceae) (Antkowiak et al, 1992), Mimosa pudica L. (Fabaceae) (Weintraub, 1952), or Sparrmannia africana L.f. (Tiliaceae) (Bünning, 1934).…”