Effects of variations in culture media and hormonal treatments upon callus induction potential in endosperm explant of Barringtonia racemosa L.

The Effect of Explant Type and 6-Benzyl Adenine (BAP) In Sapodilla (<i>Achras zapota</i>) Micropropagation

“…There are some nutrients only found in WPM medium while they are lacking in MS medium such as calcium nitrate, potassium sulfate and mangan sulfate (Saad and Elshahed, 2012). Those nutrients are likely to be the factor which influence the responses of explants towards the formation of callus (Osman et al, 2016). In the present study, the effects of using WPM medium, in bay leaf tree (Cinnamomum tamala) by Sharma and Nautiyal (2009) reported that almost all explants and plant growth regulator treatments produced compact callus and also by Avil´es et al (2009) in Juglans regia L even in endosperm explant of Barringtonia racemosa L by Osman et al (2016) Non embryogenic callus generally has lost regeneration capacity.…”

Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production

“…Thereafter, the explants were soaked in 25% of sodium hypochlorite solution added with 2-3 drops of tween 20 (Sigma, St. Louis, MO, USA) for 20 min. The explants were then thoroughly rinsed with sterile distilled water to remove any traces of remaining detergents [76].…”

“…At the end of four weeks, the formed callus was isolated from the explants. The data for callus induction in each treatment was recorded in which the morphology, fresh, and dry weights (g) [76]. The percentage of callus initiation was calculated [30].…”

Ethnobotanical Profiles and Phytochemical Constituents of Barringtonia racemosa L. for Potential Scrutiny of Bioactive Compounds through Plant Biotechnology

“…Upon callus induction percentage assessment, the highest record was identified in the culture of endosperm explant treated with 1.5 mg/l 2,4-D and 0.5 mg/l kinetin in Murashige and Skoog's (MS) medium whereby the calli formed from such treatment were found to be friable and grew intensively. In the most recent study of callus culture establishment which was published in 2016, the endosperm explant of B. racemosa was proven to be productive in yielding callus with desirable friable morphology 11 . However, the optimal combination of Plant Growth Regulators (PGR) was different as compared to the previously documented research whereby the latter found that 1.0 mg/l 2,4-D in the presence of 1.5 mg/l kinetin was the optimal treatment for callus induction.…”

“…In addition to that, the plant biotechnology approach had been incorporated in a very few studies which include the establishment of callus culture and cell suspension culture of the species [8][9][10][11] . Due to the scarcity of investigations in plant biotechnology-related areas conducted on B. racemosa, therefore, there is a great potential for the species to be further studied in such a field.…”