Many factors, most notably climate, soil, genotype and fertilising, can influence barley grain composition and its nutritive value. The aim of the research was to evaluate the impact of mineral N rate in different management systems in the static long-term experiment with three years field rotation of grain maize, wheat and barley on the chemical composition of winter barley and its nutritive value for ruminants. Crude protein content and metabolizable crude protein content were increasing with increased mineral N rates. Higher yield meant lower crude protein and metabolizable crude protein content in all mineral N rates. The average crude protein content of barley grain, presented in DLG tables (1997), which is 12.4%, was in MM and SM reached by fertilising rate 110 kg N.ha -1 . A significant impact of mineral N fertilising on crude ash, ether extract and crude fibre content could not be confirmed, nitrogen-free extract content was decreasing with increased mineral N rates. A significant impact of the management system on the Weende analysis parameters, net energy for lactation, metabolizable energy and metabolizable crude protein could not be confirmed. At both analyses a significant impact of the season was confirmed on crude protein content and nitrogen-free extract content. Although the protein content was increasing with increased mineral N rate from 9.2-14.2% (for 35%) ME content and NEL content did not significantly differ considering mineral N rate; ME.ha -1 and NEL.ha -1 increasing with increased mineral N rates was the result of the yield increasing with increased mineral N rates.