Effects of Various Cleaning Agents on the Performance of Mice in Behavioral Assays of Anxiety

Hershey, John D.; Gifford, Janace J.; Zizza, Lauren J; Pavlenko, Darya; Wagner, George C.; Miller, Shoreh

doi:10.30802/aalas-jaalas-17-000161

Cited by 12 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We ultimately replaced this with a Kaytee CritterTrail 3" Elbow Tube which led to a standard mouse housing unit (from our animal facility) which was replaced for each trial. The entire maze surface and elbow tube were thoroughly cleaned with 10% bleach solution [ 14 ] after every run and a clean empty cage unit used for every trial to prevent odor mediated cues from disrupting the assessment of learning and memory.…”

Section: Methodsmentioning

confidence: 99%

Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

et al. 2019

View full text Add to dashboard Cite

Routine general anesthesia is considered to be safe in healthy individuals. However, pre-clinical studies in mice, rats, and monkeys have repeatedly demonstrated that exposure to anesthetic agents during early post-natal periods can lead to acute neurotoxicity. More concerning, later-life defects in cognition, assessed by behavioral assays for learning and memory, have been reported. Although the potential for anesthetics to damage the neonatal brain is well-documented, the clinical significance of the pre-clinical models in which damage is induced remains quite unclear. Here, we systematically evaluate critical physiological parameters in post-natal day 7 neonatal mice exposed to 1.5% isoflurane for 2–4 hours, the most common anesthesia induced neurotoxicity paradigm in this animal model. We find that 2 or more hours of anesthesia exposure results in dramatic respiratory and metabolic changes that may limit interpretation of this paradigm to the clinical situation. Our data indicate that neonatal mouse models of AIN are not necessarily appropriate representations of human exposures.

show abstract

Section: Methodsmentioning

confidence: 99%

Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For methods relying on visual tracking, marking the animals to make them stand out from the background and also distinguishable from each other, can improve performance (Ohayon et al, 2013;Lorbach et al, 2017). However, this comes at the cost of increased human intervention and the possibility of changing animal behavior (Walker et al, 2013;Burn et al, 2008;Hurst and West, 2010;Hershey et al, 2018). Markerless techniques rely on inherent differences in animal appearance (Dell et al, 2014;Branson et al, 2009;Hong et al, 2015;Burgos-Artizzu et al, 2012;Brose et al, 2006;Unger et al, 2017;Pérez-Escudero et al, 2014;Kühl and Burghardt, 2013;Berman et al, 2014;Matsumoto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…However, animals need to go through additional preparations for these methods, adding cost of time and potential confound factors such as discomfort or inflammation. There are also concerns that these methods could alter animal behaviour (Walker et al, 2013;Burn et al, 2008;Hurst and West, 2010;Hershey et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Markings may fade over time requiring reapplication under restraint 23 and handling by humans which is time-consuming and stressful for the animals (Burn et al, 2008;Hurst and West, 2010). Certain compounds in marking agents may also alter behavior in some assays (Burn et al, 2008;Hershey et al, 2018). Furthermore, marking procedures must be determined in advance, and implemented carefully and consistently.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Markerless mouse tracking for social experiments

Sterley

Cheng

et al. 2021

Preprint

View full text Add to dashboard Cite

Automated behavior quantification requires accurate tracking of animals. Simultaneous tracking of multiple animals, particularly those lacking visual identifiers, is particularly challenging. Here we propose a markerless video-based tool to simultaneously track two socially interacting mice of the same color. It incorporates conventional handcrafted tracking and deep learning based techniques, which are trained on a small number of labeled images from a very basic, uncluttered experimental setup. The output consists of body masks and coordinates of the snout and tail-base for each mouse. The method was tested on a series of cross-setup videos recorded under commonly used experimental conditions including bedding in the cage and fiberoptic or headstage implants on the mice. Results obtained without any human intervention showed the effectiveness of the proposed approach, evidenced by a near elimination of identities switches and a 10% improvement in tracking accuracy. This suggests that the hybrid approach could be valuable for studying group behaviors, such as social interaction. This novel approach addresses problems of mistaken identities and lost information on key anatomical features that are common in existing methods. Finally, we demonstrated an application of this approach in studies of social behaviour of mice, by using it to quantify and compare interactions between pairs of mice in which some are anosmic, i.e. unable to smell. Our results indicated loss of olfaction impaired typical snout-directed social recognition behaviors of mice, while non-snout-directed social behaviours were enhanced.

show abstract

“…Grooming frequency was taken as the number of body cleaning with paws while horizontal locomotion is the number of squares crossed with four paws (Falodun et al, 2015). The cage was cleansed with 70% ethanol after each assessment to remove olfactory cue from the previous animal (Hershey et al, 2018). The experiment was carried out between 9.00 am and 4.00 pm daily to avoid changes in biological rhythm.…”

Section: Novelty-induced Rearing Grooming and Horizontal Locomotionmentioning

confidence: 99%

Neurobehavioural effects of Vigna unguiculata spp Dekindtiana (L.) Walp in mice

Adegbuyi¹,

Akanmu²,

Olayiwola³

2019

J. Drugs Pharm. Sci.

View full text Add to dashboard Cite

The study investigated the pharmacological effects of the extract and fractions of Vigna unguiculata spp dekindtiana. The neurobehavioural parameters investigated in this study include: rearing, grooming and locomotion as well as pentobarbital-induced sleeping time model as a measure of the sedative effect of the plant. The neuropharmacological profile of the plant was determined in mice. The extract and fractions were administered orally at doses of 100, 200, 400 and 800 mg/kg for novelty-induced behavior while phenobarbital-induced sleeping time was assessed at the doses of 100, 200 and 400 mg/kg. The result obtained showed significant decrease in rearing, grooming and locomotion with increase in the dose of the extract and the fractions indicating the central inhibitory effect of the plant extract. The possible mechanism of action of the novelty-induced behaviours was investigated by using picrotoxin (GABAA antagonist), cyproheptadine (5HT2 antagonist), yohimbine (alpha2 adrenergic antagonist). The result of the study showed the reversal of the novelty-induced rearing and locomotion with picrotoxin while the reduction in novelty-induced behaviour (rearing and locomotion) was potentiated by both cyproheptadine and yohimbine. This shows the possibility of GABAergic, serotonergic and adrenergic involvement in the behavioural effects of the extract and its fractions. Also, the methanol extract and ethyl acetate fraction showed dose-dependent increase in sleeping time and dose-dependent decrease in sleep latency indicating the hypnotic effect of the extract of the plant. The possible mechanism of action of the phenobarbital-induced sleeping time was investigated using flumazenil (an antagonist of GABA), where a reversal was obtained suggesting the possible involvement of GABAergic receptor. In conclusion, the result showed that methanol extract and its fractions exhibited both central inhibitory effect and hypnotic effect in mice.

show abstract

Effects of Various Cleaning Agents on the Performance of Mice in Behavioral Assays of Anxiety

Cited by 12 publications

References 21 publications

Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse

Markerless mouse tracking for social experiments

Neurobehavioural effects of Vigna unguiculata spp Dekindtiana (L.) Walp in mice

Contact Info

Product

Resources

About