Effects of various diluents on the quality and shelf life of Donggala bull semen

Abstract

This study aimed to determine the effects of different semen diluent on the quality and storage time of liquid semen of Donggala bull. Semen was obtained from four selected bulls which collected using artificial vagina. The semen diluent is based on Tris aminomethane-citric acid with different concentration of glucose namely P1=0.00g, P2=0.25g, P3=0.50g, and P4=0.75g. Another group of treatment also prepared based on Tris aminomethane-glucose with different concentration of egg yolk namely P5=0%, P6=15%, P7=20%, and P8=25%. The data obtained were then analyzed descriptively. Results showed tris aminomethane-citric acid-glucose diluents had sperm progressive motile at >40% until the day 4 of storage, and tris aminomethane-glucose-egg yolk only able to support sperm life for 2 days, and in P8 group shows 41.02% motile on the day 3. In this study, we found the pattern of the increasing of shelf life affect to the decrease rate of sperm viability and normality.

Pengaruh Penambahan Glukosa pada Pengencer Masa Kriopreservasi

“…Perlakuan yang menghasilkan persentase motilitas paling tinggi setelah pengenceran yaitu kontrol dan glukosa 1%. Hal ini tidak sesuai dengan penelian sebelumnya yang mengatakan (Mirajuddin et al, 2021) bahwa penambahan glukosa dalam pengencer cenderung menurunkan motilitas progresif spermatozoa sapi Donggala yang disimpan selama 3 hari.…”

The Effect of Sugarcane Water Subtitution as a Candidate Substitute for Fructose in CEP Diluent on Spermatozoa Quality of Friesian Holstein Bull during Frozen Storage

“…Glukosa dan fruktosa merupakan salah satu bahan sintetis yang harganya relatif mahal. Untuk itu diperlukan bahan alternatif yang lebih murah, mudah didapat, memenuhi syarat sebagai pengencer dan tidak mengandung zat toksik bagi spermatozoa (Duma et al, 2021). Salah satu bahan organik alternatif yang mudah didapat serta terjangkau adalah air tebu.…”