2008
DOI: 10.4162/nrp.2008.2.4.317
|View full text |Cite
|
Sign up to set email alerts
|

Effects of various metal ions on the gene expression of iron exporter ferroportin-1 in J774 macrophages

Abstract: Macrophages play a key role in iron metabolism by recycling iron through erythrophagocytosis. Ferroportin-1 (FPN1) is a transporter protein that is known to mediate iron export from macrophages. Since divalent metals often interact with iron metabolism, we examined if divalent metals could regulate the expression of FPN1 in macrophages. J774 macrophage cells were treated with copper, manganese, zinc, or cobalt at 10, 50, or 100 µM for 16 to 24 h. Then, FPN1 mRNA and protein levels were determined by quantitati… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
7
0
1

Year Published

2010
2010
2022
2022

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 13 publications
(8 citation statements)
references
References 28 publications
0
7
0
1
Order By: Relevance
“…FPN1 mRNA is increased in J774 cells in response to copper but not zinc or manganese. 26 In Caco-2 cells and as shown here in cultured mouse bone marrow macrophages, FPN1 mRNA levels increase in response to a wide variety of transition metals including zinc, copper, manganese, cobalt, and cadmium.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…FPN1 mRNA is increased in J774 cells in response to copper but not zinc or manganese. 26 In Caco-2 cells and as shown here in cultured mouse bone marrow macrophages, FPN1 mRNA levels increase in response to a wide variety of transition metals including zinc, copper, manganese, cobalt, and cadmium.…”
Section: Discussionmentioning
confidence: 99%
“…FPN1 mRNA is increased in J774 cells in response to copper but not zinc or manganese. 26 In Caco-2 cells and as shown here in cultured mouse bone marrow macrophages, FPN1 mRNA levels increase in response to a wide variety of transition metals including zinc, copper, manganese, cobalt, and cadmium.Cells change FPN1 mRNA levels in response to these metals, however, there is a clear difference in the mechanisms that underlie this response. Our data indicate that zinc and cadmium induce FPN1 transcription through the action of MFT-1.…”
mentioning
confidence: 99%
“…However, whether or not Fpn exports Mn and plays a role in Mn homeostasis remain controversial. While some studies report the induction of Fpn protein by Mn exposure (134,135), other reports indicate treatment with Mn has no effect on gene expression (136). Xenopus laevis oocytes expressing human Fpn showed lower intracellular Mn and higher extracellular Mn (135).…”
Section: Mn Efflux: Ferroportin Slc30a10 Ncxmentioning
confidence: 99%
“…39 Treatment with manganese 40,41 and other metals 42 has been shown to regulate Fpn expression. In macrophages, Fpn expression is altered by exposure to manganese, 43 Hepcidin is also present in alveolar macrophages, 38,44 but the possible role for Fpn in manganese transport, its speculative function in the respiratory uptake of the metal and/or its detoxification, and the potential role for hepcidin in the regulation of these processes remain to be fully explored.…”
Section: Ferroportin (Slc40a1)mentioning
confidence: 99%