Effects of Various Preextraction Treatments of Crinum asiaticum Leaf on Its Anti-Inflammatory Activity and Chemical Properties

Abstract

Crinum asiaticum Linn. has been used in Thai traditional medicine to relieve inflammatory symptoms and treat osteoarthritis. There have been reports on its potent anti-inflammatory property but nothing on the effects of different pretreatments on its chemical properties and anti-inflammatory activity. Pretreatment of herbal raw materials is an important step which affects the overall quality of Thai traditional medicine. The objectives of this study were to investigate different treatments of C. asiaticum leaves prior to ethanolic extraction and to compare the extracts for their anti-inflammatory activity and chemical properties. The treatments included hot air drying in an oven, microwave drying, traditional grilling on a charcoal stove before drying in an oven, and temperature shock in hot and cold water before hot air drying. The anti-inflammatory activity and chemical properties of the extracts were analyzed using the established methods. Results showed that 95% ethanolic extract of hot air oven-dried leaves had the highest anti-inflammatory activity and total phenolic and lycorine contents. We recommend hot air drying as a preextraction treatment for C. asiaticum leaves for its simplicity, best retention of the herbal quality, and suitability for scaling up to an industrial process.

Formulation design and physicochemical evaluation of an anti-inflammatory hydrogel patch containing Crinum asiaticum L. extract

“…The HPLC system (Agilent ® 1200; Agilent Technologies, USA) and C18 reverse phase column (Zorbax ® C18, 4.6 × 250 mm, 5 µ) were used to analyze lycorine content according to the method of Kongkwamcharoen and co-workers ( 11 ) and detected by diode array detector at the wavelength of 290 nm.…”

“…Crinum asiaticum L. (Amaryllidaceae family) has long been used in Thailand and Asia to relieve pain in inflamed joints as an inflammatory disease treatment ( 8 9 ). Previous research has delineated the bioactivity of C. asiaticum L. which includes antioxidant and anti-inflammatory activity ( 10 11 12 ) and also showed a significant anti-inflammatory effect in vivo as measured by inhibition of paw edema 51.60 ± 2.50% at the first hour and 40.80 ± 0.52% at the fourth hour after administration ( 13 ). Lycorine ( Fig.…”

The Phytochemistry and Pharmacology of Tulbaghia, Allium, Crinum and Cyrtanthus: ‘Talented’ Taxa from the Amaryllidaceae

“…The extract was found to possess pro-healing effects when topically applied on animal models by influencing various stages of healing process [ 322 ]. C asiaticum extract and norgalanthamine potentially influenced hair growth via inhibition of 5α-reductase activity and TGF-β1-induced canonical pathway [ 39 , 314 ]. There is a report on the inhibitory effects of three C. asiaticum genotypes against key enzymes implicated in the pathogenesis of Alzheimer’s disease and diabetes [ 319 ].…”

“…Given the aforementioned biological activities, Allium , Tulbaghia , Cyrtanthus and Crinum are utilized in traditional medicinal therapy for varying diseases and conditions [ 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. For example, Allium is used as concoctions, decoctions, extracts, and herbal preparations to treat angina, amoebic dysentery, arthritis, cardiovascular diseases, cholera, catarrh, dysmenorrhea, fever, headaches, hepatitis, stomach disorders, throat infections, and prostatic hypertrophy [ 30 , 31 , 35 , 36 , 37 , 38 ].…”

Ethnomedicine of Medicinal Plants Used by Traditional Healers to Facilitate Bone Injury Healing in West Kalimantan, Indonesia

“…Some of the anti-inflammatory mechanisms of curcumin include suppression of COX-2 expression and nitric oxide generation, also by blocking the activation of inflammatory mediators of NF-ĸB through inhibition of AKT and IĸBα kinases (Boroumand et al, 2018;Jacob et al, 2007). Lycorine is the main phytochemical contents in C. asiaticum that responsible for anti-inflammatory activities (Kongkwamcharoen et al, 2021).…”