Effects of various salts and of mannitol on ion and proline accumulation in relation to osmotic adjustment in rice (Oryza Sativa L.) callus cultures

Lutts, S.; Kinet, Jean‐Marie; Bouharmont, J.

doi:10.1016/s0176-1617(96)80193-3

Cited by 106 publications

(77 citation statements)

References 31 publications

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“…The phenomena might be occurred due to reduction of water availability and lose of turgor pressure (TP) in the cells of the calli. Such physiological causes were reported in previous investigation for Oryza sativa, 47 Carthamus tinctorius, 46,48 Saccharum sp., 9 Tagetes minuta 49 and Triticum durum 50,51 . Errabi et al 9 mentioned that due to interference of Na C and Cl -ions on uptake and translocation processes, nutritional imbalance might be created and the growth of callus is declined.…”

Section: Discussionsupporting

confidence: 75%

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Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

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Section: Discussionsupporting

confidence: 75%

“…As a result an imbalance of essential ions be created and cell of salt sensitive varieties could not be survived. Such reports previously mentioned in rice, 47,54 sugarcane, 9,55 Carthamus tinctorius 46 and Cynara cardunculus.…”

Section: Discussionmentioning

confidence: 51%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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“…Bajji et al (1998) showed accumulation of proline with increase of NaCl concentration in Atriplex halimus L. callus, but with a concomitant decrease in growth rate. Others authors, such Lutts et al (1996), suggested that accumulation of proline in Oryza sativa L. was a symptom of damage, rather than an adaptative response as an intracellular osmotic adjustment.…”

Section: Resultsmentioning

confidence: 99%

Alleviation of Salt Stress Using Exogenous Proline on a Citrus Cell Line

Lima‐Costa¹,

Ferreira²,

Duarte³

et al. 2010

Acta Hortic.

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“…In vitro culture is a valid and rapid tool for studying the behavior of plants in response to salt stress, while other factors (nutrients, lighting, temperature) are held constant and controlled in an optimal manner. This approach has been successfully used for the evaluation of salt stress in brassica [8], tomato [9][10][11][12][13][14][15][16], mulberry [17,18], potato [19], rice [20][21][22], poplar [23,24], strawberry [25], eucalyptus [26][27][28], grapevine [29,30], citrus [31,32], and pistacia [33].…”

Section: Introductionmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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In order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

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Effects of various salts and of mannitol on ion and proline accumulation in relation to osmotic adjustment in rice (Oryza Sativa L.) callus cultures

Cited by 106 publications

References 31 publications

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Alleviation of Salt Stress Using Exogenous Proline on a Citrus Cell Line

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

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