Effects of various solvent concentration, liquid-solid ratio, temperatures and time values on the extraction yield of anthocyanin from Vietnam Hibiscus sabdariffa L. (Roselle)

Abstract

Nowadays, the trends in human health and fossil fuel dependent markets present opportunities for agricultural crops as renewable sources in several substitutes of synthetic components in clothing, fuels, and food. This research focused on anthocyanins along with protocatechuic acid and quercetin, which have been recognized as bioactive compounds in Hibiscus sabdariffa L. aqueous extracts. Laboratory extraction study was conducted on various solvent concentration, liquid-solid ratio, temperatures and time values on the extraction yield of anthocyanin from Vietnam Hibiscus sabdariffa L. (Roselle). On theother hand, total anthocyanins wereinvestigated in Hibiscus sabdariffa L. extract as μg/mL cyanidin-3-glucoside (cyd-3-glu). Extraction temperature of 60°C using solvent concentration 50%, extraction time of 30 min at liquid-solid ratio of 8:1 showed the highest anthocyanin recovery at 180,821 mg/L. Overall, Vietnam Hibiscus sabdariffa L. (Roselle) are responsive to anthocyanin extraction, making it an appropriate substrate for the development of industrial colorants and dyes.

Process kinetics optimization for extraction of essential oil from the peel of Citrus sinensis for the development of food packaging film

“…Thus, a high solid‐to‐liquid ratio could encourage a growing concentration gradient, resulting in diffusion rate increase and promoting more solvent extraction of solids. Once equilibrium is established, the likelihood of bio‐active components coming into touch with the extracting solvent will not increase any more (Nhut Pham et al, 2019). This assertion is in agreement with our findings.…”

Optimization of stirring-assisted extraction of anthocyanins from purple roselle (Hibiscus sabdariffa L.) calyces as pharmaceutical and food colorants

“…In this case, the compound will be kept inside the crude drug's vacuole. However, if the crude drug-solvent combination reaches a certain ratio, plant cells will absorb the solvent rapidly and swell to the point of bursting, where the compound is simultaneously released inside the vacuole [13]. Therefore, the S/L ratio of 1:15 g/mL was chosen for the next step of extraction.…”

“…A suitable extraction technology that is applicable on an industrial scale should, however, be developed first to utilize roselle calyces as a source of natural dyes. Several anthocyanin extraction methods from the calyces are heat-assisted extraction (HAE), ultrasoundassisted extraction (UAE), maceration, infusion, microwave-assisted extraction (MAE), and supercritical fluid extraction (SFE) [9,[11][12][13][14][15][16].…”

“…Although the extraction of anthocyanins from roselle calyx has been studied in previous researches [12][13][14]18], this is the first work to optimize the SAE process and evaluate its efficiency against UAE. Therefore, the objectives of this research were threefold: To optimize the type of extraction solvent (50%, 70%, 95% ethanol, 50% ethanol acidified with citric acid, or 50% ethanol acidified with tartaric acid), extraction time (10,15, or 20 min), and the S/L ratio (1:5, 1:10, or 1:15 g/mL) in the SAE of anthocyanins from roselle calyces.…”