Effects of various treatments of bovine complement on its lytic efficacy measured by two different tests

Nielsen, Klaus; Rosenbaum, B.; Ballinger, Royce Ε.; Stiller, Jenny

doi:10.1016/0165-2427(84)90053-9

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…It must be stressed however that our test predominantly detects the alternative complement pathway (9,11,15); in fact (AMADORI and AGNOLETTI, unpublished observations), we could verify that:…”

Section: Discussionmentioning

confidence: 62%

Characterization of some Parameters of Non‐Specific Immunity in Beef Cattle (II)

Ponti

Amadori

Agnoletti

et al. 1989

Journal of Veterinary Medicine, Series B

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Serum protein, immunoglobulins, complement, lysozyme, serum bactericidal activity and blast transformation of peripheral blood lymphocytes were tested in beef cattle in different herds and age groups.These parameters were evaluated in their time-kinetics, in comparison with age-matched groups of dairy cattle. Like dairy calves, beef calves up to 4 months of age were shown to have low levels of serum bactericidal activity and haemolytic complement; besides, a worse profile was detected in serum protein and y 2 globulin levels. AI(OH), and oil-adjuvanted vaccines had a favourable influence on immunoglobulin and lysozyme synthesis. An acute stressing event like transportation was shown to decrease mitogen-driven lymphocyte stimulation for at least 10 days after arrival.

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Section: Discussionmentioning

confidence: 62%

Characterization of some Parameters of Non‐Specific Immunity in Beef Cattle (II)

Ponti

Amadori

Agnoletti

et al. 1989

Journal of Veterinary Medicine, Series B

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“…The complement pathway involved in killing was determined by treating serum with EGTA-MgCl2. Since virtually no killing activity remained after EGTA-MgCl2 treatment, it is likely that classical pathway activation was responsible (47,48). This is consistent with data showing that B. abortus LPS does not activate the alternative pathway of complement (20).…”

Section: Discussionmentioning

confidence: 99%

Killing of Brucella abortus by bovine serum

et al. 1988

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Studies of the serum bactericidal system in bovine brucellosis were undertaken to investigate the role of the humoral immune response in protection of cattle against the facultative intracellular parasite Brucella abortus. Fresh sera from normal control cattle, infected cattle, and cattle immunized with B. abortus cell envelopes were collected before treatment and during the course of immunization or infection. Normal fresh bovine serum or fresh agammaglobulinemic serum from colostrum-deprived calves was effective in killing smooth virulent B. abortus 2308, but rough strains RB51 (a rough mutant of strain 2308) and 45/20 were much more senitive to serum. The difference in susceptibility to serum was shown to be correlated with differences in lipopolysaccharide chemotype, with the more resistant strain 2308 having 0 polysaccharide and the more susceptible strains 45/20 and RB51 lacking 0 side chains. By treatment of fresh serum with MgCl2 and EGTA [ethylene glycol-bis(IO-aminoethyl ether)-N,N,N',N'-tetraacetic acid] killing was shown to occur via the classical pathway of complement activation. When antibody to B. abortus was present, killing of strain RB51 increased but killing of smooth strain 2308 decreased. The earliest antibody response in serum from infected animals did not interfere with killing. When affinity-purified bovine immunoglobulins specific for B. abortus smooth lipopolysaccharide were added to fresh normal bovine serum, immunoglobulin Gl (IgGl) and IgG2 isotypes blocked killing but IgM and IgA isotypes did not. Thus, it appears that serum from previously unexposed animals or animals early during infection can kill smooth B. abortus, an appropriate defense mechanism before the organism becomes intracellular. At later stages of infection, blocking antibodies predominate.

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Defences of the bovine mammary gland against infection and prospects for their enhancement

Craven¹,

Williams²

1985

Veterinary Immunology and Immunopathology

141

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Effects of various treatments of bovine complement on its lytic efficacy measured by two different tests

Cited by 7 publications

References 8 publications

Characterization of some Parameters of Non‐Specific Immunity in Beef Cattle (II)

Characterization of some Parameters of Non‐Specific Immunity in Beef Cattle (II)

Killing of Brucella abortus by bovine serum

Defences of the bovine mammary gland against infection and prospects for their enhancement

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