Effects of Varying the Chemical Composition of the Stationary Phase on the Separation of Certain C₁₉,and C₂₁,and C₂₇ Steroids by Gas Chromatography

Gas-Liquid Chromatography of Sterol Methyl Ethers and Some Correlations Between Molecular Structure and Retention Data*

“…While this work was in progress it was reported (Vanden Heuvel et al, 1961) that sterol trifluoroacetates, chromatographed on neopentyl glycol succinate, had retention times comparable with those of the methyl ethers (an observation which has been confirmed in these laboratories: Lasser and Clayton, unpublished work). Several reports have also appeared of the use of various types of polar liquid phases for the gas-liquid chromatography of sterols and steroid hormones (Haahti et al, 1961a,b;Vanden Heuvel et al, 1961a,c; Lipsky and Landowne, 1961). In agreement with the findings described here, these reports all indicate the superiority of the polar phases as compared with the less polar silicone and hydrocarbon phases for effecting separations based on the more subtle structural differences between molecular species.…”

The correlation of gas-liquid chromatographic behaviour and structure of steroids

“…These observations again illustrate the different character of the two stationary phases, SE-30 responding essentially to molecular skeletal size and shape, and QF-1 interacting selectively with substituent groups. The elution of 3ac-hydroxy-5oc-androstan-17-one acetate before its 5k-isomer on a polyester column (Lipsky & Landowne, 1961) may now be construed as a result of selective retention of the equatorial acetoxyl group in the latter compound.…”

Analysis of drugs and related compounds by capillary column GLC

“…The earliest reported attempts to chromatograph steroids on open tubular columns were those of Chen and Lantz [ l ] and Landowne and Lipsky [2] in 1961. The results these workers obtained with stainless steel columns were not satisfactory.…”