Effects of vascular endothelial growth factor on porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer

Biswas, Dibyendu; Jung, Eui‐Man; Jeung, Eui Bae; Hyun, Sang‐Hwan

doi:10.1016/j.theriogenology.2010.08.012

Cited by 27 publications

(20 citation statements)

References 41 publications

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“…Oocyte collection and in vitro maturation: Retrieval and IVM of porcine oocytes from local slaughterhouse-derived ovaries were performed as we described previously [4,26]. Briefly, cumulus oocyte complexes (COCs) were aspirated using an 18-gauge needle attached to a 10 ml disposable syringe from superficial follicles 3 to 6 mm in diameters, pooled in 15 ml conical tubes and allowed to settle down as sediment for 5 min at 37°C.…”

Section: Chemicalsmentioning

confidence: 99%

“…Donor cell preparation: Donor cell preparation was performed same as described previously [4]. Fibroblasts were isolated from fetuses at day 40 of gestation.…”

Section: Chemicalsmentioning

confidence: 99%

“…These molecules include leukemia inhibitory factor (LIF) [1], insulin-like growth factor-I (IGF-I) [46], epidermal growth factor (EGF) [45], transforming growth factor beta (TGF-β) [16] and vascular endothelial growth factor [4]. Most of these growth factors and regulatory molecules are derived from or produced by the endometrium or embryo itself.…”

mentioning

confidence: 99%

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Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Kwak

Cheong

Jeon

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNTderived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (P<0.05) increased blastocyst formation and total cell number in blastocysts compared with the control (12.3% and 41.4 vs. 9.0% and 34.7, respectively). However, there was no effect on cleavage rate. The numbers of cells in the inner cell mass and trophectoderm were significantly higher in the pGM-CSF treatment group (6.0 and 43.0, respectively) compared with the control (4.4 and 31.9, respectively). Treatment with 10 ng/ml pGM-CSF significantly increased POU5F1 and Cdx2 mRNA expression in blastocysts. In addition, Bcl-2, Dnmt1 and proliferating cell nuclear antigen (PCNA) mRNA expression were upregulated in blastocysts in the pGM-CSF supplemented group compared with the control. These results suggest that pGM-CSF improves the quality and developmental viability of porcine SCNT embryos by regulating transcription factor expression.

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Section: Chemicalsmentioning

confidence: 99%

“…Donor cell preparation: Donor cell preparation was performed same as described previously [4]. Fibroblasts were isolated from fetuses at day 40 of gestation.…”

Section: Chemicalsmentioning

confidence: 99%

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Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Kwak

Cheong

Jeon

et al. 2012

The Journal of Veterinary Medical Science

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“…The inclusion criteria were: [1] age ≤37 years; [2] the first time to undergo IVF cycle; [3] male-factor infertility; [4] basal FSH (day 3) level <10 mIU/mL; [5] both ovaries present. The exclusion criteria were: [1] any diseases that could affect ovary function, such as ovarian neoplasm, polycystic ovary syndrome, hyperprolactinemia, endometriosis and premature ovarian failure; [2] use of steroids within 3 months; [3] chronic diseases, thyroid and adrenal diseases. According to whether pregnant or not, the women were then subdivided into two groups: pregnant group and non-pregnant group.…”

Section: Subjectsmentioning

confidence: 99%

“…Many studies suggest it is important to acquire high-quality oocytes for the success of IVF. The mechanism of oocyte maturation is complicated, involving not only the hormone regulation of hypothalamuspituitary-ovary axis, but also modulation of clusters of growth factors in ovary microenvironment, such as epidermal growth factor (EGF), insulin-like growth factor (IGF-I), and vascular endothelial growth factor (VEGF) [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Brain-derived neurotrophic factor from follicular fluid is positively associated with rate of mature ooocytes collected and cleavage rate in intracytoplasmic sperm injection patients

Wang¹,

Sun²,

Zhen³

et al. 2011

J Assist Reprod Genet

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Purpose The aim of the study was to evaluate the correlation between BDNF and oocyte maturation and to verify whether BDNF could predict in vitro fertilization (IVF) outcome. Methods The follicle fluid (FF) for BDNF, E 2 and P assay were obtained from 59 patients undergoing intracytoplasmic sperm injection (ICSI). The women were divided into two groups by pregnancy outcome and their clinical and lab data were compared. And the correlation of BDNF with E 2 , P, age, and IVF data were analyzed. Results Positive correlation was observed between BDNF and E 2 concentration in FF. BDNF was positively correlated with the rate of mature oocytes collected and cleavage rate. Conclusions The BDNF in FF could not predict IVF outcome, but BDNF in FF might play an important role in the maturation of oocyte and development of oocyte into preimplantation embryo.

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Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

Anchordoquy

Testa

Sirini

et al. 2015

Cell Biology International

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The objective of this study was to investigate the effect of VEGF and Cysteamine during in vitro maturation (IVM) of bovine oocytes on GSH content and developmental competence. For this purpose, experiments were designed to evaluate the effect of 0, 100, 300, and 500 ng/mL VEGF in IVM medium on: GSH content in oocytes and cumulus cells (Exp. 1) and subsequent embryo development (Exp. 2). Also, influence of adding 500 ng/mL VEGF and 100 μM Cysteamine to IVM medium on GSH content in oocytes and cumulus cells (Exp. 3) and oocyte developmental capacity (Exp. 4) were evaluated. Oocytes were matured in: a) Control; b) VEGF 0-3 h; c) Cysteamine 4-24 h; d) VEGF 0-3 h + Cysteamine 4-24 h; and e) VEGF + Cysteamine 24 h. The results showed that: i) VEGF did not alter GSH content in oocytes and cumulus cells; (ii) supplementation of 300 and 500 ng/mL VEGF increased blastocyst yield; (iii) the presence of VEGF + Cysteamine simultaneously during 24 h improved GSH content but not embryo development; and (iv) the presence of VEGF during the first 3 h + Cysteamine from 4 to 24 h increased GSH concentrations and subsequent embryo development. In conclusion, the addition of VEGF and Cysteamine in two sequential steps to maturation medium result in an improvement of cytoplasmic maturation, with a positive impact on oocyte developmental capacity by increasing the efficiency of in vitro blastocyst production. However, the effect was detrimental when both VEGF and Cysteamine were present during 24 of IVM.

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Effects of vascular endothelial growth factor on porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer

Cited by 27 publications

References 41 publications

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Brain-derived neurotrophic factor from follicular fluid is positively associated with rate of mature ooocytes collected and cleavage rate in intracytoplasmic sperm injection patients

Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

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