Effects of Vasoactive Intestinal Peptide on Steroid Secretion and Plasminogen Activator Activity in Granulosa Cells of the Hen1

Johnson, A. L.; Tilly, J L

doi:10.1095/biolreprod38.2.296

Cited by 43 publications

(24 citation statements)

References 29 publications

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“…Several studies have demonstrated that VIP influences important ovarian functions, such as regulation of steroidogenesis [Ahmed et al, 1986], cAMP accumulation [Tornell et al, 1988], plasminogen activator production [Johnson and Tilly, 1988] and oocyte maturation [Tornell et al, 1988].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, many of the effects of VIP on ovarian granulosa cells are similar to the action of gonadotropins [Ahmed et al, 1986;Johnson and Tilly, 1988;Johnson et al, 1994], suggesting that VIP may be important for regulating gonadotropinindependent development and ovarian follicle survival. These data confirm previous findings concerning the participation of sympathetic innervation in the control of follicular development.…”

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confidence: 99%

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Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture

Bruno

Celestino²,

Lima-Verde³

et al. 2009

Cells Tissues Organs

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The aim of this study was to evaluate the effect of vasoactive intestinal peptide (VIP)on the survival, activation and growth of goat preantral follicles after in vitro culture. The ovarian cortex was divided into small pieces and one fragment was immediately fixed (control). The remaining fragments were cultured in vitro for 1 or 7 days at 39°C and 5% CO₂, in supplemented minimum essential medium (MEM⁺) with or without different concentrations of VIP (1, 10, 50, 100 or 200 ng/ml). Noncultured (fresh control) and cultured ovarian fragments were processed for histological analysis and transmission electron microscopy. Follicles were classified as primordial or developing, and as normal or degenerated. Our findings indicate that when compared with control, addition of all concentrations of VIP except 200 ng/ml resulted in similar percentages of normal preantral follicles after 1 and 7 days of culture. Culture of ovarian cortex tissue for 1 and 7 days increased the percentage of follicular activation in all treatments when compared with control, except with 1 ng/ml of VIP after 1 day. However, no difference was observed between VIP-treated and MEM⁺-treated follicles. In addition, after 7 days of culture, the highest follicular and oocyte diameters were observed in follicles cultured with 10 ng/ml VIP relative to MEM⁺ alone. Transmission electron microscopy showed ultrastructural integrity of follicles after 7 days of culture in 10 ng/ml VIP. In conclusion, this study demonstrates that VIP maintains follicular integrity and stimulates caprine preantral follicle growth.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture

Bruno

Celestino²,

Lima-Verde³

et al. 2009

Cells Tissues Organs

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“…VIP, originally considered to be a gut hormone, has recently been found to increase tPA activity in cultured rat GCs and cumulusoocyte complexes [21,60]. The ovulatory effect of VIP was also studied using in-vitroperfused ovaries from immature rats primed with PMSG.…”

Section: Gnrh Fsh and Vip Stimulate Oocyte And Gc Tpa Activity And Imentioning

confidence: 99%

“…Rat GCs express tPA in response to treatment with gonadotropins, such as FSH and LH [101,102], GnRH [20] and VIP [21,60]. tPA can also be induced with growth factors [59,[102][103][104], such as EGF, FGF, TGF-· and bFGF.…”

Section: Hormonal Regulation Of Tpa Gene Expression In the Ovarymentioning

confidence: 99%

Regulation of the Plasminogen Activator System in the Ovary

Liu¹

1999

Neurosignals

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Extracellular matrix (ECM) not only provides a structural support for the organism, but also actively conducts cell-to-cell signal transduction and regulates cell proliferation, migration, development and metabolism. The targeted ECM degradation generated by plasminogen activator (PA) and regulated by plasminogen activator inhibitor (PAI) is, therefore, an event that affects a wide variety of physiological and pathological processes. The ovary is the best model to study the regulation and function of extracellular proteolysis mediated by multicomponents like the PA system. Studies carried out over the past 10 years in a number of laboratories have elucidated some of the biochemical events related to the function and regulation of the PA system in the ovary: hormone-induced proteolytic activity provided by tissue-type PA(tPA) and modulated by PAI-1 in the preovulatory follicles is responsible for a controlled and directed proteolysis leading to rupture of selected follicles during ovulation, whereas the coordinated expression of urokinase-type PA (uPA) and PAI-1 in the early growing follicle may be important in ECM degradation during cell proliferation and migration; the PA system may also play a role in the control of corpus luteum (CL) development through an autocrine or paracrine mechanism. Increase in tPA and PAI-1 expression in CL at a later stage is well correlated with a sharp decrease in CL progesterone production, while the increase in uPA mRNA levels and activity in the early stage of CL development is correlated with an increase in progesterone secretion.

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“…Our previous studies have demonstrated that GCs and oocyte of rat and rhesus monkey produced tPA, while theca cells produced PAI-1, the coordinated expression of tPA and PAI-1 regulated by gonadotrophins in the ovary-induced ovulation [6,7]. Evidence has also shown that rat GCs in vitro express tPA in response to FSH, LH, GnRH, VIP, and growth factors via different signaling pathways [4,[8][9][10][11][12]. GnRH is likely to stimulate tPA mRNA level through protein kinase C pathway [13], while growth factors EGF and TGF-alpha induced tPA mRNA and activity in GCs through a pathway independent of protein kinase A and C [14].…”

Section: Introductionmentioning

confidence: 99%

Mitogen-activated protein kinase regulates FSH-induced expression of tissue-type plasminogen activator through an activator protein 1 response element

Yang

et al. 2008

Endocr

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We have analyzed a possible role of mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) in the regulation of FSH-induced tissue type plasminogen activator (tPA) production in granulosa cells (GCs) prepared from DES-treated immature rats; Treatment of the cells in the presence of FSH with MAPK inhibitors, such as UO126 or SB203580, significantly decreased the FSH-induced tPA production, suggesting that multiple signaling pathways may be involved in FSH-regulated tPA expression. We further examined possible signaling action involved in FSH-activated ERK1/2 and p38 MAPK on tPA production, and observed that FSH receptor occupancy led to both ERK1/2 and p38 MAPK phosphorylation. Such action might be through a protein kinase A-dependent pathway because the observed activation was destroyed by the addition of its specific inhibitor H89 to the culture. The inhibition of ERK1/2 and p38 MAPK activation by their specific inhibitors remarkably reduced FSH-induced tPA mRNA and its protein production. We further examined whether AP-1 located in the tPA promoter is involved in FSH-regulated tPA production, and demonstrated that FSH significantly stimulated AP-1 expression, whereas inclusion of H89, UO126, or SB20358 in the culture significantly decreased FSH-induced AP-1 expression. In summary, FSH-induced ERK1/2 and p38 MAPK activation is capable of regulating tPA production in cultured primary GCs, and that the transcript factor AP-1 may be important in the regulation of FSH-induced tPA expression.

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Effects of Vasoactive Intestinal Peptide on Steroid Secretion and Plasminogen Activator Activity in Granulosa Cells of the Hen1

Cited by 43 publications

References 29 publications

Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture

Vasoactive Intestinal Peptide Improves the Survival and Development of Caprine Preantral Follicles after in vitro Tissue Culture

Regulation of the Plasminogen Activator System in the Ovary

Mitogen-activated protein kinase regulates FSH-induced expression of tissue-type plasminogen activator through an activator protein 1 response element

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