Effects of Vegetation Restoration on Soil Bacterial Communities, Enzyme Activities, and Nutrients of Reconstructed Soil in a Mining Area on the Loess Plateau, China

Abstract

Soil microbes are the main driving forces and influencing factors of biochemical reactions in the environment. Study of ecological recovery after mining activities has prompted wider recognition of the importance of microbial diversity to ecosystem recovery; however, the response of soil bacterial communities to vegetation restoration types and soil biochemical properties remains poorly understood. The purpose of this research was to explore the soil bacterial communities and soil biochemical properties at four sampling sites (brushland (BL), forestland (FL), grassland (GL) and unreclaimed land (UL)) on the Loess Plateau, China, to evaluate the effect of vegetation restoration on the reconstructed soil in mining areas. In August 2017, samples were collected at the Heidaigou coal mine dumps. Illumina MiSeq sequencing was used to identify the structure of the soil bacterial community and evaluate its relationships with soil biochemical properties. The results showed that soil biochemical properties (soil organic matter, available phosphorus, urease, sucrase, microbial biomass carbon and microbial biomass nitrogen) were significantly increased in BL, FL and GL relative to UL, indicating that the soil quality was significantly improved by vegetation restoration. In addition, the results showed that the vegetation restoration on the reconstructed soil in the mining area could significantly improve the operational taxonomic units (OTUs), abundance (ACE and Chao1) and diversity (Shannon and Simpson) indices of bacterial community and the dominant phyla were Proteobacteria, Actinobacteria and Acidobacteria. With vegetation restoration, the relative abundance of Proteobacteria and Acidobacteria showed an increasing trend, while that of Actinobacteria showed a decreasing trend, and the dominant phyla were only significantly correlated with a few biochemical properties. Moreover, there were no changes in soil bacterial community structures across the four sampling sites and the response of the bacterial community to biochemical properties was not obvious. This implies that, although the region has experienced about 20 years of vegetation restoration, the microbial community still maintains good stability and lagging response to soil biochemical properties. Since the BL soil had better biochemical properties and higher bacterial richness and diversity, it was recommended as the optimum vegetation restoration type for soil reclamation in this area.

Impact of Land Use Types and Seasonal Variations on Soil Physico-chemical Properties and Microbial Biomass Dynamics in a Tropical Climate, Ghana

“…The present study, land uses impacted on microbial biomass and generally increased in the order of degraded mine land < agricultural land < forest land, confirming our first and second hypothesis. This was consistent with other studies indicating that soil microbial biomass decreased when forested lands were converted to agricultural land or degraded by tillage implements [45,44].…”

“…The microbial biomass has been recognised as a key component of soil microbial activity influencing many soil processes [43]. They characteristically impact on soil quality thereby influencing the vegetation in a given area [44]. The microbial biomass is however, sensitive to environmental changes as well as land-use conversion.…”

Rhizosphere Soil Microbial Properties on Tetraena mongolica in the Arid and Semi-Arid Regions, China

“…Among the bacteria from various topsoil sources, 92% belong to the 9 dominant phyla: Proteobacteria, Acidobacteria, Actinobacteria, Verrucomicrobia, Bacteroidetes, Chloroflexi, Planctomycetes, Gemmatimonadetes, and Firmicutes [ 31 ]. Li studied the effects of vegetation restoration among different soil bacterial communities, and the dominant phyla were Proteobacteria, Actinobacteria, and Acidobacteria [ 7 ]. Li [ 32 ] also investigated the diversity and composition of bacterial communities in response to reclamation of a soil subsidence area affected by mining activities, and found that the predominant phyla in present in the soils were Proteobacteria, Actinobacteria, Acidobacteria, and Planctomycetes.…”

“…Coal mining activities affect the physical quality of the soil: high bulk density and low water holding capacity, porosity, moisture content, pH, and soil nutrients (total nitrogen and available phosphorus) [ 6 ]. Coal mining activities interfered with the soil bacterial community structure, which led to a significant reduction in soil enzyme activity, with the dominant bacteria comprising Proteobacteria, Actinomycetes, and Acidobacteria [ 7 ].…”

Erratum: Li, P.; Zhang, X.; et al. Effects of Vegetation Restoration on Soil Bacterial Communities, Enzyme Activities, and Nutrients of Reconstructed Soil in a Mining Area on the Loess Plateau, China. Sustainability 2019, 11, 2295

“…The authors would like to make the following corrections about the published paper [1]. The changes are as follows:…”