Background: The transmission of vibration from a single-axis tractor to the human body is determined by its dynamic response; this, in turn, depends on the physical characteristics of a person's hand, the contact area, the strength of the grip, the push force of the tractor, and the position. The purpose of this work was to measure and evaluate handgrip strength and fatigue resistance for operators of a 15 horsepower single-axle tractor before and after vibration exposure. Methods: Grip strength of dominant and non-dominant hands before and after 30 minutes of tilling operations was measured and recorded. The operators performed tilling tasks with the tractor in third gear, while the vibration levels were measured at the tractor handle along with the vertical, forward, and transverse directions. Results: The average operator grip strength was 33.6 ± 2.7 and 26.3 ± 3.3 kg and the average overall grip strength declined from 39.7 to 29.31 kg, although the average fatigue strength was 27.6 and 26.5 seconds for the dominant hand before and after vibration exposure. For the non-dominant hand, the mean grip strength measured was 28.7±2.9 and 23.1±1.9 kg and the maximum grip strength was 32.79 and 26.25, while fatigue was 29.76 and 22 seconds before and after tilling respectively.Conclusion: The average reduction in grip strength suggest considerable differences in grip strength for dominant and non-dominant hands of single axle tractor operators and shows that vibration transmitted from the single axle tractor handle has a major effect on the operators.