Effects of Vibrio harveyi infection on serum biochemical parameters and expression profiles of interleukin-17 (IL-17) / interleukin-17 receptor (IL-17R) genes in spotted sea bass

Mao, Xuebin; Tian, Yuan; Wen, Haishen; Liu, Yang; Sun, Yalong; Yanglang, Arat; Li, Yun

doi:10.1016/j.dci.2020.103731

Cited by 19 publications

(9 citation statements)

References 43 publications

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“…Total protein (TP) in serum is synthesized by the liver and includes albumin (ALB) and globulin (GLB) ( Sepulveda et al., 2012 ). Significant reduction in TP, ALB, and GLB were observed in studies of Streptococcus lactis -free infected tilapia , and Vibrio harveyi infected sea bass ( Mao et al., 2020 ; Zhou et al., 2020 ). Herein, similar changes were observed in the serum proteins of hybrid sturgeon after D-59 infection, which might be closely related to the effect of S. sciuri infection on protein synthesis in the liver.…”

Section: Discussionmentioning

confidence: 93%

Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki

Zhang

Xue

Xiao

et al. 2022

Front. Cell. Infect. Microbiol.

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Hybrid sturgeon is the main species of sturgeon cultured in China, with the advantages of a fast growth rate, early sexual maturity, fertile offspring, and more stable genetic traits. In May 2021, a large number of deaths characterized by superficial hemorrhage and liver damage occurred in a sturgeon farm in Yichang, Hubei Province, which posed a significant risk to hybrid sturgeon captive breeding. We isolated a pathogenic bacterium named D-59 from the diseased sturgeon with apparent symptoms. The pathogen was identified as Staphylococcus sciuri using 16S rRNA gene phylogenetic analysis combined with biochemical identification. Regression experiments showed that D-59 exhibited clinical signs similar to those of diseased sturgeon in the farm after intraperitoneal injection into hybrid sturgeon. High-throughput sequencing of gut microbes in D-59-infected sturgeon showed that the number of gut microbial species decreased in infected sturgeon, the number of some intestinal commensal bacteria decreased, and the balance of the intestinal microorganisms was disrupted. Histopathological sections indicated many inflammatory cells, congestion, and even necrosis in the tissue of diseased sturgeon. Analysis of blood indexes revealed an increase in the proportion of mononuclear cells and a decrease in the proportion of lymphocytes in the peripheral blood of diseased sturgeon. Significantly elevated serum levels of aspartate aminotransferase and alanine aminotransferase, whereas alkaline phosphatase, total protein, albumin, and globulin were decreased in diseased sturgeon. Antimicrobial susceptibility tests demonstrated that D-59 is susceptible to florfenicol, enrofloxacin, and neomycin sulfate. This study aimed to highlight the dangers of Staphylococcus sciuri infection during hybrid sturgeon culture and to provide recommendations for diagnosis and treatment.

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Section: Discussionmentioning

confidence: 93%

Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki

Zhang

Xue

Xiao

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Furthermore, the composition of the gut microbiome of IL-17A/F1-KO medaka was different than that of wild-type (WT) medaka, indicating that IL-17A/F signaling controls the gut microbiome through the induction of various genes, apart from immunity-related genes 15 . Data from other studies also showed that bacterial infection can induce the expression of teleosts IL-17 ligands in the intestine 16,17 . Furthermore, teleost recombinant teleost IL-17A/F induced immune genes such as il1β, il6, and β-defensin (defb3) in vitro, as has been observed in mammals [18][19][20] .…”

Section: Introductionmentioning

confidence: 80%

Interleukin-17 Receptor A1 Gene Knockout Causes Weight Loss and Reduction of Intestinal Metabolism-Related Genes in The Japanese Medaka, Oryzias Latipes

Okamura¹,

Miyanishi²,

Kinoshita³

et al. 2020

Preprint

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In the intestine, the host must be able to control the gut microbiota and efficiently absorb transiently supplied metabolites, at the risk of enormous infection. In mammals, the inflammatory cytokine interleukin (IL)-17A/F is one of the key mediators in the intestinal immune system. However, many functions of IL-17 in vertebrate intestines remain unclarified. In this study, we established a gene-knockout (KO) model of IL-17 receptor A1 (RA1), an IL-17A/F receptor, in Japanese medaka (Oryzias latipes) using genome editing technique and the phenotypes were compared to wild type (WT) based on transcriptome analyses. Upon hatching, homozygous IL-17RA1-KO medaka mutants showed no significant morphological abnormality. However, after 4 months, significant weight decreases and reduced survival rates were observed in IL-17RA1-KO medaka. Comparing gene-expression patterns in WT and IL-17RA1-KO medaka revealed that various metabolism- and immune-related genes were significantly down-regulated in IL-17RA1-KO medaka intestine, particularly genes related to mevalonate metabolism (mvda, acat2, hmgcs1, and hmgcra) and genes related to IL-17 signaling (such as il17c, il17a/f1, and rorc) were found to be decreased. These findings show that IL-17RA regulated immune- and various metabolism-related genes in the intestine for maintaining the health of Japanese medaka.

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“…These previous data revealed the importance of IL-17A/F1 in controlling microbiota and for the induction of not only AMPs, but also expression of digestive enzyme encoding genes in the medaka intestine 16 . Data from further studies have also shown that bacterial infection induces the expression of teleost IL-17 ligands in the intestine 17 , 18 . Furthermore, recombinant teleost IL-17A/F show induced expression of immune genes, such as IL1Β , IL6 , and DEFB3 (β-defensin) in vitro, as has been observed in mammals 19 – 21 .…”

Section: Introductionmentioning

confidence: 95%

A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

Okamura

Miyanishi

Kinoshita

et al. 2021

Sci Rep

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In the intestine, the host must be able to control the gut microbiota and efficiently absorb transiently supplied metabolites, at the risk of enormous infection. In mammals, the inflammatory cytokine interleukin (IL)-17A/F is one of the key mediators in the intestinal immune system. However, many functions of IL-17 in vertebrate intestines remain unclarified. In this study, we established a gene-knockout (KO) model of IL-17 receptor A1 (IL-17RA1, an IL-17A/F receptor) in Japanese medaka (Oryzias latipes) using genome editing technique, and the phenotypes were compared to wild type (WT) based on transcriptome analyses. Upon hatching, homozygous IL-17RA1-KO medaka mutants showed no significant morphological abnormality. However, after 4 months, significant weight decreases and reduced survival rates were observed in IL-17RA1-KO medaka. Comparison of gene-expression patterns in WT and IL-17RA1-KO medaka revealed that various metabolism- and immune-related genes were significantly down-regulated in IL-17RA1-KO medaka intestine, particularly genes related to mevalonate metabolism (mvda, acat2, hmgcs1, and hmgcra) and genes related to IL-17 signaling (such as il17c, il17a/f1, and rorc) were found to be decreased. Conversely, expression of genes related to cardiovascular system development, including fli1a, sox7, and notch1b in the anterior intestine, and that of genes related to oxidation–reduction processes including ugp2a, aoc1, and nos1 in posterior intestine was up-regulated in IL-17RA1-KO medaka. These findings show that IL-17RA regulated immune- and various metabolism-related genes in the intestine for maintaining the health of Japanese medaka.

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Effects of Vibrio harveyi infection on serum biochemical parameters and expression profiles of interleukin-17 (IL-17) / interleukin-17 receptor (IL-17R) genes in spotted sea bass

Cited by 19 publications

References 43 publications

Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki

Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki

Interleukin-17 Receptor A1 Gene Knockout Causes Weight Loss and Reduction of Intestinal Metabolism-Related Genes in The Japanese Medaka, Oryzias Latipes

A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

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