Effects of Vigorous Blending on Yield and Quality of Protein Isolates Extracted From Cottonseed and Soy Flours

He, Zhongqi; Cao, Heping; Cheng, H. N.; Zou, Haixuan; Hunt, James F.

doi:10.5539/mas.v7n10p79

Cited by 41 publications

(32 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein content of CSPw was 64.4% which was lower than the protein content of laboratory-made CSPw (He et al, 2013a;He et al, 2015). The product CSPa was basically protein-dominated, more like the total protein isolate CSPI, which is also observed with the laboratory-made CSPa and CSPI (He et al, 2013a;He et al, 2015). Oil was an impurity (2.5%) in the staring material CSM (Table 1).…”

Section: Major Components Of Pilot Produced Cottonseed Meal Productsmentioning

confidence: 77%

“…The portion of CSPw accounted only for 0.7% of CSM mass. The low yield of CSPw was partially due to the gyssopol effect as Berardi et al (1969) observed higher CSPw yield with glandless (without gossypol) cottonseed meal than glanded (with gossypol) meal, even though the CSPw could be extracted with more vigorous blending (He et al, 2013a). The solid content in uncollected soluble fractions WSF and ASF accounted for 15.8% and 12.3% of the initial sample mass, respectively, resulting in the 64.0% of total CSM mass recovered with the pilot-scale production trial.…”

Section: Mass Yield and Protein Recoverymentioning

confidence: 99%

“…CSPI was obtained by the procedure of 0.027 M NaOH extraction and 1 M HCl precipitation (pH 5.0) adapted from literature (Berardi et al, 1969;He et al, 2013a;Zhang et al, 2009). Duplicate experiments were performed.…”

Section: Pilot Workmentioning

confidence: 99%

See 2 more Smart Citations

Pilot-Scale Production of Washed Cottonseed Meal and Co-Products

He¹,

Klasson²,

Wang³

et al. 2015

MAS

Self Cite

View full text Add to dashboard Cite

Enhanced utilization of defatted cottonseed meal (CSM)-based products as biobased raw materials would increase the profitability of cotton growers and processors. In this work, a multiple-step procedure that can be used to produce water washed cottonseed meal (WCSM) and a co-product water soluble cottonseed protein (CSPw) from CSM was tested at pilot scale. Alternatively, the procedure can also be used to produce CSPw, alkali soluble protein (CSPa) and the protein extracted insoluble residue (CSIR). The chemical composition of these pilot-produced products was generally comparable to that of the corresponding laboratory-prepared products. The fiber content was higher in the pilot-produced WCSM than in laboratory-produced product mainly due to the higher fiber content in the mill-based starting meal material used in the pilot trial. The protein content in the pilot-produced CSPw was only 64.4% of the solid matter, indicating that additional rinse is needed before drying the HCl-precipitated CSPw fraction. Per the yield and composition data, it was concluded that it was feasible to produce the four products WCSM, CSPw, CSPa, and CSIR from CSM in a large scale. Thus, adoption of this procedure would provide the necessary quantities for exploring the practical utilization of these products in biomaterial industry.

show abstract

Section: Major Components Of Pilot Produced Cottonseed Meal Productsmentioning

confidence: 77%

Section: Mass Yield and Protein Recoverymentioning

confidence: 99%

See 1 more Smart Citation

Pilot-Scale Production of Washed Cottonseed Meal and Co-Products

He¹,

Klasson²,

Wang³

et al. 2015

MAS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Whereas seed protein isolates have been widely evaluated as potential wood adhesives, the isolation procedure involves corrosive alkaline and acidic reagents and it makes isolate-based adhesives relatively expensive and less environment-friendly 16 . Thus, some defatted seed meals (flours) with or without treatment have also been tested for the adhesive purpose, even though the adhesive properties of these meals do not perform as well as protein isolates [17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

“…2. Obtain the working meal by grinding the solid defatted meal in a cyclone sample mill to pass a 0.5 mm steel screen 16 . 3.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Testing of Plant Seed Meal-based Wood Adhesives

Chapital

2015

JoVE

Self Cite

View full text Add to dashboard Cite

Recently, the interest in plant seed meal-based products as wood adhesives has steadily increased, as these plant raw materials are considered renewable and environment-friendly. These natural products may serve as alternatives to petroleum-based adhesives to ease environmental and sustainability concerns. This work demonstrates the preparation and testing of the plant seed-based wood adhesives using cottonseed and soy meal as raw materials. In addition to untreated meals, water washed meals and protein isolates are prepared and tested. Adhesive slurries are prepared by mixing a freeze-dried meal product with deionized water (3:25 w/w) for 2 hr. Each adhesive preparation is applied to one end of 2 wood veneer strips using a brush. The tacky adhesive coated areas of the wood veneer strips are lapped and glued by hot-pressing. Adhesive strength is reported as the shear strength of the bonded wood specimen at break. Water resistance of the adhesives is measured by the change in shear strength of the bonded wood specimens at break after water soaking. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for substitution of synthetic-based wood adhesives. Adjustments to the adhesive formulation with or without additives and bonding conditions could optimize their adhesive properties for various practical applications. Video LinkThe video component of this article can be found at

show abstract

Quantitative comparison of the storage protein distribution in glandless and glanded cottonseeds

Zhang

Mattison

2022

Agricultural & Env Letters

Self Cite

View full text Add to dashboard Cite

Glanded (Gd) cottonseed (Gossypium hirsutum L.) contains scattered gossypol glands. Glandless (Gl) cottonseed is a new type of seed containing only trace levels of gossypol. This work quantitatively compared the content and migration pattern of Gd and Gl protein isolates. Both protein samples were subjected to sodium dodecyl sulfate (SDS)-gel electrophoresis, and the protein gel bands were separated into seven partitions for peptide mass spectroscopic analysis. While multiple peptide fragments (isoformers) of vicilin and legumin proteins were present in both samples, the percentage of vicilins in total seed protein was higher in Gd (74.9%) than in Gl (63.4%). In contrast, legumin proteins were more abundant in Gl (30.4%) than Gd (23.6%).Minor protein components such as lipid-related oleosins and vicilin-like antimicrobial peptides 2-2 were also observed at a relatively higher incidence in Gl compared with Gd, potentially reflecting a need for increased protein-related defense capability in the absence of gossypol against natural predators or adverse growth environment.

show abstract

Effects of Vigorous Blending on Yield and Quality of Protein Isolates Extracted From Cottonseed and Soy Flours

Cited by 41 publications

References 20 publications

Pilot-Scale Production of Washed Cottonseed Meal and Co-Products

Pilot-Scale Production of Washed Cottonseed Meal and Co-Products

Preparation and Testing of Plant Seed Meal-based Wood Adhesives

Quantitative comparison of the storage protein distribution in glandless and glanded cottonseeds

Contact Info

Product

Resources

About