Effects of vipoxin and its components on HepG2 cells

Doumanov, Jordan; Mladenova, Kirilka; Topouzova-Hristova, Tanya; Stoitsova, Stoyanka; Petrova, Svetla

doi:10.1016/j.toxicon.2014.12.009

Cited by 12 publications

(5 citation statements)

References 41 publications

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“…Many toxins isolated from snake venoms have been used as tools for understanding different pathophysiological events, since these proteins present several biological effects such as antiparasitic, antimicrobial and antitumor activities [ 22 – 24 ]. In that context, snake venom PLA 2 s of both acidic and basic character, and also synthetic peptides derived from homologous PLA 2 s, have been extensively studied due to their wide variety of pharmacological effects, including their antitumor and antiangiogenic properties [ 25 – 27 ].…”

Section: Discussionmentioning

confidence: 99%

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Silva¹,

Costa²,

Paiva³

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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BackgroundPhospholipases A2 (PLA2s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA2 isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines.MethodsThe cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry technique was used for the analysis of cell cycle alterations and death mechanisms (apoptosis and/or necrosis) induced in tumor cells after treatment with BthTX-I.ResultsIt was observed that BthTX-I was cytotoxic to all evaluated tumor cell lines, reducing their viability in 40 to 50 %. The myotoxin showed modulating effects on the cell cycle of PC-12 and B16F10 cells, promoting delay in the G0/G1 phase. Additionally, flow cytometry analysis indicated cell death mainly by apoptosis. B16F10 was more susceptible to the effects of BthTX-I, with ~40 % of the cells analyzed in apoptosis, followed by HepG2 (~35 %), PC-12 (~25 %) and HL-60 (~4 %).ConclusionsThese results suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer.

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Section: Discussionmentioning

confidence: 99%

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Silva¹,

Costa²,

Paiva³

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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“…In recent years, the search for new antimicrobial agents from natural sources has intensified, such as those from microorganisms , algae , plants , along with anuran , scorpion , spider , wasp , bee and snake venoms that would constitute an important treatment alternative against innumerable emerging or non‐emerging infectious diseases that afflict society.…”

mentioning

confidence: 99%

Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX, a Lys49 Phospholipase A₂ Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)

Diniz-Sousa

Caldeira

Kayano

et al. 2017

Basic Clin Pharma Tox

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Snake venom phospholipases A (PLA s) are responsible for numerous pathophysiological effects in snakebites; however, their biochemical properties favour antimicrobial actions against different pathogens, thus constituting a true source of potential microbicidal agents. This study describes the isolation of a Lys49 PLA homologue from Lachesis muta muta venom using two chromatographic steps: size exclusion and reverse phase. The protein showed a molecular mass of 13,889 Da and was devoid of phospholipase activity on an artificial substrate. The primary structure made it possible to identify an unpublished protein from L. m. muta venom, named LmutTX, that presented high identity with other Lys49 PLA s from bothropic venoms. Synthetic peptides designed from LmutTX were evaluated for their cytotoxic and antimicrobial activities. LmutTX was cytotoxic against C2C12 myotubes at concentrations of at least 200 μg/mL, whereas the peptides showed a low cytolytic effect. LmutTX showed antibacterial activity against Gram-positive and Gram-negative bacteria; however, S. aureusATCC 29213 and MRSA strains were more sensitive to the toxin's action. Synthetic peptides were tested on S. aureus, MRSA and P. aeruginosaATCC 27853 strains, showing promising results. This study describes for the first time the isolation of a Lys49 PLA from Lachesis snake venom and shows that peptides from specific regions of the sequence may constitute new sources of molecules with biotechnological potential.

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“…The compounds were administered in doses ranging from 0.35 to 2.88 μM and the effect was dose dependent compared with the negative control. The phospholipase A 2 component was much less genotoxic, although it triggered apoptosis [ 48 ].…”

Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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Envenomation by animal venoms remains a serious medical and social problem, especially in tropical countries. On the other hand, animal venoms are widely used as a source of biologically active compounds for the development of novel drugs. Numerous derivatives of animal venoms are already used in clinical practice. When analysing the mechanisms of action of animal venoms, attention is usually focused on the main target of the venom’s enzymes and peptides such as neurotoxic, cytotoxic or haemorrhagic effects. In the present review, we would like to draw attention to the “hidden” effects of animal venoms and their derivatives in regard to DNA damage and/or protection against DNA damage. Alkaloids and terpenoids isolated from sponges such as avarol, ingenamine G or variolin B manifest the capability to bind DNA in vitro and produce DNA breaks. Trabectidin, isolated from a sea squirt, also binds and damages DNA. A similar action is possible for peptides isolated from bee and wasp venoms such as mastoparan, melectin and melittin. However, DNA lesions produced by the crude venoms of jellyfish, scorpions, spiders and snakes arise as a consequence of cell membrane damage and the subsequent oxidative stress, whereas certain animal venoms or their components produce a genoprotective effect. Current research data point to the possibility of using animal venoms and their components in the development of various potential therapeutic agents; however, before their possible clinical use the route of injection, molecular target, mechanism of action, exact dosage, possible side effects and other fundamental parameters should be further investigated.

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Effects of vipoxin and its components on HepG2 cells

Cited by 12 publications

References 41 publications

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX, a Lys49 Phospholipase A₂ Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

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Effects of vipoxin and its components on HepG2 cells

Cited by 12 publications

References 41 publications

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX, a Lys49 Phospholipase A2 Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

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Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX, a Lys49 Phospholipase A₂ Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)