Effects of visible light-irradiated camphorquinone and 9-fluorenone on murine oral mucosa

Okada, Naoya; Muraoka, Eitoku; Fujisawa, Seiichiro; Machino, Mitsuo

doi:10.4012/dmj.27.809

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…Second, the subsequent addition of the photocrosslinker CQ, as a radical initiator added to the derivatized gelatin solution, was subjected to irradiation to produce a transparent and stiff scaffold (tube and membrane) without degrading the incorporated growth factors or ECM (membrane only). (The photocrosslinker CQ has been used in the dental field to prepare composites for humans without toxic side effects [45]). For all these reasons, these tubes will be translatable to human SCI repair [46].…”

Section: Discussionmentioning

confidence: 99%

“…Neurotrophin was detected in the culture medium by ELISA upon release from the membranes in vitro up to 21 days. Higher levels of rhBDNF were found when SCs were present, suggesting that SCs themselves released rhBDNF as well as the membranes [45, 46] and providing, at least in part, an explanation for improved cell survival and proliferation.…”

Section: Discussionmentioning

confidence: 99%

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Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Sazo

Maenaka

et al. 2012

Biomaterials

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One of the most exciting new avenues of research to repair the injured spinal cord is to combine cells for implantation with scaffolds that protect the cells and release growth factors to improve their survival and promote host axonal regeneration. To realize this goal, we fabricated biodegradable, photocurable gelatin tubes and membranes for exploratory in vitro studies. Detailed methods are described for their fabrication with a high gelatin concentration. Gelatin membranes fabricated in the same way as tubes and photo-co-immobilized with rhBDNF or rhNT-3, with or without Schwann cells (SCs), showed an initial burst of neurotrophin release within 24h, with release diminishing progressively for 21 days thereafter. SCs attained their typical bipolar conformation on membranes without neurotrophins but adhesion, alignment and proliferation were improved with neurotrophins, particularly rhBDNF. When dorsal root ganglion explants were cultured on membranes containing laminin and fibronectin plus both neurotrophins, neurite outgrowth was lengthier compared to combining one neurotrophin with laminin and fibronectin. Thus, these gelatin membranes allow SC survival and effectively release growth factors and harbor extracellular matrix components to improve cell survival and neurite growth. These scaffolds, based on the combination of cross-linked gelatin technology and incorporation of neurotrophins and extracellular matrix components, are promising candidates for spinal cord repair.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Sazo

Maenaka

et al. 2012

Biomaterials

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“…It can be seen from our results (Figure 2) that the bonding agents (XP Bond, and Adper Scotchbond SE) containing three of the mentioned toxic components (TEGDMA, UDMA, HEMA) were also found to be the most cytotoxic of the 7 bonding agents. Furthermore, XP Bond, and Adper Scotchbond SE in addition also contained the photo-initiator camphorquinone which is reported to be cytotoxic [34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells

Olivier¹,

Grobler²,

Osman³

2012

OJST

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Today it is generally accepted that most bonding agents are cytotoxic. In this study the relative cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells were investigated. Materials and Methods. Near-confluent mouse 3T3 fibroblast cells were exposed to Dulbecco Modified Eagle’s Medium containing extractions from the seven different bonding agents. The cell survival rate was then determined using the standard MTT assay. Results. The cell survival rate ranking is: iBond (94%) < Gbond (78%) < Xeno V (71%) < Adper Easy Bond (63%) < Xeno V+ (61%) < Adper Scotchbond SE (33%) < XP Bond (32%). Part A of Adper Scotchbond SE had a survival rate of 35% and part B 38%. These two parts did not differ significantly. Adper Scotchbond SE and XP Bond do not differ significantly. While Xeno V+, Xeno V and Adper Easy Bond do not differ. (p < 5%; Tukey-Kramer Multiple-Comparison Test). Conclusion. All of the tested adhesive bonding agents were cytotoxic with survival rate of 3T3 cells between 94% to 31%. Of the 7 bonding agents tested iBond was found to be only slightly toxic and by far the least toxic. The two bonding agents (XP Bond and Adper Scotchbond SE) containing UDMA plus TEGDMA plus HEMA plus camphorquinone were found to be the most toxic

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“…It is used in the manufacture of antimalaria drugs and other pharmaceuticals. 9F may cause phototoxic reactions in vitro and in vivo , in particular in the oral mucosa (36, 37). Beyond that, little is known about adverse reactions to 9F, in particular regarding skin.…”

Section: Discussionmentioning

confidence: 99%

Black tattoo inks are a source of problematic substances such as dibutyl phthalate

et al. 2011

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The sensitizing agent dibutyl phthalate acts directly on keratinocytes and can drive Th2 responses following skin exposure via induction of thymic stromal lymphopoietin gene expression. Hexachloro-1,3-butadiene is genotoxic in vitro and 9-fluorenone is cytotoxic, generating reactive oxygen species under light exposure. The substances found in the inks might be partially responsible for adverse skin reactions to tattoos.

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Effects of visible light-irradiated camphorquinone and 9-fluorenone on murine oral mucosa

Cited by 7 publications

References 13 publications

Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells

Black tattoo inks are a source of problematic substances such as dibutyl phthalate

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