Effects of Vitamin a Depletion in Young Adults

Brenner, Sadie

doi:10.1001/archinte.1943.00210040033004

Cited by 24 publications

(3 citation statements)

References 17 publications

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“…Clausen, Baum, McCoord, Rydeen & Breese (1940) claimed that ethanol mobilized vitamin A in dogs and later the same group of workers (Clausen, Breese, Baum, McCoord & Rydeen, 1941) stated that drinking liquor to the extent of 'the usual "social" evening common in this country' (U.S.A.) raised the vitamin A plasma levels of humans. Brenner & Roberts (1943) also found a rise in humans. However, not only have British investigators failed to observe any rise after administration of ethanol (Yudkin, 1941;Hume & Krebs, 1949), but the increase recorded by the American investigators was almost entirely due to esterified vitamin A (Clausen, Baum, McCoord, Rydeen & Breese, 1942), so that any such rise would apparently serve no useful therapeutic purpose.…”

Section: Resultsmentioning

confidence: 90%

Studies in vitamin A. 14. The alleged mobilization of vitamin A by adrenaline

Goodwin

Wilson

1949

Biochemical Journal

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Section: Resultsmentioning

confidence: 90%

Studies in vitamin A. 14. The alleged mobilization of vitamin A by adrenaline

Goodwin

Wilson

1949

Biochemical Journal

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“…months [6][7][8][9] to years [10]. Secondly, subjects that develop night blindness may be cured within hours or took months to return to normal.…”

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confidence: 99%

Night Blindness May Weaken Your Bones

Khan¹

2017

PPIJ

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“…They included housewives, office workers, factory hands of all types, and labourers, and came from all walks of life. They were tested only when free from infection, as Brenner and Roberts (1943) found that feverish colds reduced the blood level of vitamin A by one-half. All specimens of blood were collected at approximately three hours after 1500 DEC. 31, 1949 VITAMIN A AND TOTAL CAROTENOIDS IN PLASMA a meal, but no attempt was made to obtain fasting specimens, since Kimble (1939) found that ordinary meals do not cause a measurable increase in either vitamin A or carotene within two to six hours.…”

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confidence: 99%

Vitamin A, Total Carotenoids, and Thymol Turbidity Levels in Plasma

Campbell¹,

Tonks²

1949

BMJ

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In the course of a previous investigation into the cause of nystagmus in coal-miners we estimated the levels of vitamin A and total carotenoids in the plasma (Campbell and Tonks, 1948). Thymol turbidity tests were performed at the same time on a number of cases in order to exclude the possibility of liver dysfunction as a cause of fluctuation in these constituents. Since the diet of the majority of people had been rather drastically altered by war and postwar conditions in Great Britain, we were obliged to establish "normal" figures for comparison. It was thought that the values obtained might be of practical interest to other investigators engaged on similar problems. Methods Estination of Vitamin A and Total Carotenoids in PlasmaClausen's application of the Carr-Price colorimeter method was the means used in this procedure. The colour was measured in a Hilger absorptiometer, using filters specially designed for these estimations. The results were read off from standard curves prepared as suggested by Yudkin (1941).The following reagents were used: (1) From these readings graphs were constructed so that results could be ascertained with accuracy and speed.Two millilitres of plasma was placed in a stoppered centrifuge tube, 2 ml. of ethyl alcohol was added slowly, and the contents thoroughly mixed. To this mixture was added 4 ml. of petroleum ether. The stopper was inserted and the tube shaken at frequent intervals for half an hour. The mixture was then allowed to settle into its two layers or was centrifuged. An aliquot of the top layer (usually 3.6 ml.) was then transferred to an absorption cell, made up to 5 ml. with petroleum ether, and a reading taken using the carotenoid filter. The equivalent value in terms of a-carotene was then obtained from the standard curves and calculated for 100 ml. of plasma.The petroleum ether solution in the cell was evaporated to dryness by placing the cell in a bath of warm water (40-60°C.). A gentle current of dry air or nitrogen was used to assist evaporation. The residue was dissolved in 0.5 ml. of dry chloroform. With the use of the special vitamin-A filter the cell was placed in the rack of the absorptiometer and 5 ml. of antimony trichloride solution added. The reading was taken within 10 seconds of the addition of the reagent.The amount of vitamin A present in the cell was calculated by reading the value from the standard curve for vitamin A and subtracting the amount of blue colour due to the reaction of the antimony trichloride with the estimated amount of carotenoids present. From this value the amount in 100 ml. of plasma was calculated. Thymol Turbidity TestThis test was originally described by Maclagan (1944), but in order to get more precise comparable values the modification of Ley, Lewis, and Davidson (1946) Roberts (1943) found that feverish colds reduced the blood level of vitamin A by one-half. All specimens of blood were collected at approximately three hours after 1500 DEC. 31, 1949 VITAMIN A AND TOTAL CAROTENOIDS IN PLASMA a meal, but no attempt was m...

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Effects of Vitamin a Depletion in Young Adults

Cited by 24 publications

References 17 publications

Studies in vitamin A. 14. The alleged mobilization of vitamin A by adrenaline

Studies in vitamin A. 14. The alleged mobilization of vitamin A by adrenaline

Night Blindness May Weaken Your Bones

Vitamin A, Total Carotenoids, and Thymol Turbidity Levels in Plasma

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