Effects of Vitamin B2 Supplementation in Broilers Microbiota and Metabolome

Biagi, Elena; Mengucci, Carlo; Barone, Monica; Picone, Gianfranco; Lucchi, Alex; Celi, Pietro; Litta, Gilberto; Candela, Marco; Manfreda, Gerardo; Brigidi, Patrizia; Capozzi, Francesco; Cesare, Alessandra De

doi:10.3390/microorganisms8081134

Cited by 14 publications

(18 citation statements)

References 59 publications

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“…Total bacterial DNA was extracted from each stool sample using the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) with previously described modifications [38]. Briefly, 500 μl of the feces and storage medium mix were resuspended in a lysis buffer (500 mM NaCl, 50 mM Tris-HCl pH 8, 50 mM EDTA, 4 % (w/v) SDS).…”

Section: Dna Extraction From Fecesmentioning

confidence: 99%

Fecal Microbiota Monitoring in Elite Soccer Players Along the 2019–2020 Competitive Season

Viciani¹,

Barone

Bongiovanni

et al. 2022

Int J Sports Med

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Physical exercise affects the human gut microbiota that, in turn, influences athletes’ performance. The current understanding of how the microbiota of professional athletes changes along with different phases of training is sparse. We aim to characterize the fecal microbiota in elite soccer players along with different phases of a competitive season using 16S rRNA gene sequencing. Fecal samples were collected after the summer off-season period, the pre-season retreat, the first half of the competitive season, and the 8 weeks COVID-19 lockdown that interrupted the season 2019-2020. According to our results, the gut microbiota of professional athletes changes along with the phases of the season, characterized by different training, diet, nutritional surveillance, and environment sharing. Pre-season retreat, during which nutritional surveillance and exercise intensity were at their peak, caused a decrease in bacterial groups related to unhealthy lifestyle and an increase in health-promoting symbionts. The competitive season and forced interruption affected other features of the athletes’ microbiota, i.e. bacterial groups that respond to dietary fibers load and stress levels. Our longitudinal study, focusing on one of the most followed sports worldwide, provides baseline data for future comparisons and microbiome-targeting interventions aimed at developing personalized training and nutrition plans for performances maximization.

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Section: Dna Extraction From Fecesmentioning

confidence: 99%

Fecal Microbiota Monitoring in Elite Soccer Players Along the 2019–2020 Competitive Season

Viciani¹,

Barone

Bongiovanni

et al. 2022

Int J Sports Med

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“…Frontiers in Microbiology 15 frontiersin.org selectively increase the number of the bacteria, so that the probiotics effect is more significant and lasting. A study analyzed the cecal metabolome of broilers, fed diet supplemented with vitamin B2, found two significant different metabolites of interest, namely short-chain fatty acids (acetic acid, propionic acid, lactic acid, lactic acid, succinic acid and butyl Acid) and metabolites related to energy metabolism (aspartic acid, glutamic acid, niacin, formic acid and pyruvate; Biagi et al, 2020). Therefore, synbiotics has the metabolite characteristics of both probiotics and prebiotics, and is also related to energy metabolism.…”

Section: Metabolites Of Synbioticsmentioning

confidence: 99%

How to employ metabolomic analysis to research on functions of prebiotics and probiotics in poultry gut health?

Zuo

Maiorano

et al. 2022

Front. Microbiol.

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Gut health can be considered one of the major, manageable constituents of the animal immunity and performance. The fast spread of intestinal diseases, and increase of antimicrobial resistance have been observed, therefore the intestinal health has become not only economically relevant, but also highly important subject addressing the interest of public health. It is expected, that the strategies to control infections should be based on development of natural immunity in animals and producing resilient flocks using natural solutions, whilst eliminating antibiotics and veterinary medicinal products from action. Probiotics and prebiotics have been favored, because they have potential to directly or indirectly optimize intestinal health by manipulating the metabolism of the intestinal tract, including the microbiota. Studying the metabolome of probiotics and gut environment, both in vivo, or using the in vitro models, is required to attain the scientific understanding about the functions of bioactive compounds in development of gut health and life lasting immunity. There is a practical need to identify new metabolites being the key bioactive agents regulating biochemical pathways of systems associated with gut (gut-associated axes). Technological advancement in metabolomics studies, and increasing access to the powerful analytical platforms have paved a way to implement metabolomics in exploration of the effects of prebiotics and probiotics on the intestinal health of poultry. In this article, the basic principles of metabolomics in research involving probiotics and probiotics are introduced, together with the overview of existing strategies and suggestions of their use to study metabolome in poultry.

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“…High-quality reads were binned into operational taxonomic units (OTUs) according to the taxonomic threshold of 97% using UCLUST (Edgar, 2010). The 97%-similarity threshold allowed us to obtain groups of sequences, possibly ascribable to species or small group of species, that could play specific ecological roles in the ecosystem, as previously reported (Biagi et al, 2020). The taxonomy was assigned using the RDP classifier against the Greengenes database (release May 2013).…”

Section: Bioinformatics and Statisticsmentioning

confidence: 99%

Impact of Plastic Debris on the Gut Microbiota of Caretta caretta From Northwestern Adriatic Sea

et al. 2021

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Plastic pollution is nowadays a relevant threat for the ecological balance in marine ecosystems. Small plastic debris (PD) can enter food webs through various marine organisms, with possible consequences on their physiology and health. The loggerhead sea turtle (Caretta caretta), widespread across the whole Mediterranean Sea, is a “flagship species,” useful as indicator of the general pollution level of marine ecosystems. Ingested PD accumulate in the final section of turtles’ digestive tract before excretion. During their transit and accumulation, PD also interact with the residing microbial community, with possible feedback consequences on the host’s health. To explore the possible relationship between fecal microbial composition and PD ingestion, we collected fecal samples from 45 turtles rescued between 2017 and 2019 in the Northwestern Adriatic Sea (Italy), assessing occurrence and content of PD in the samples and in parallel the microbiome structure by 16S rRNA gene sequencing. According to our findings, almost all samples contained PD, mirroring the high level of plastic pollution in the area. We identified phylotypes associated to a high amount of PD, namely Cetobacterium somerae and other taxa, possibly responding to contamination by plastic-associated chemicals. Furthermore, putative marine pathogens were found associated to higher plastic contamination, supporting the hypothesis that PD can act as a carrier for environmental pathogenic bacteria into marine organisms. Besides confirming the role of the sea turtle as relevant flagship species for plastic pollution of the marine environment, our study paves the way to the exploration of the impact that PD ingestion can have on the microbial counterpart of large marine organisms, with potential feedback consequences on the animal and ecosystem health.

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Effects of Vitamin B2 Supplementation in Broilers Microbiota and Metabolome

Cited by 14 publications

References 59 publications

Fecal Microbiota Monitoring in Elite Soccer Players Along the 2019–2020 Competitive Season

Fecal Microbiota Monitoring in Elite Soccer Players Along the 2019–2020 Competitive Season

How to employ metabolomic analysis to research on functions of prebiotics and probiotics in poultry gut health?

Impact of Plastic Debris on the Gut Microbiota of Caretta caretta From Northwestern Adriatic Sea

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