Effects of Vitamin E on the Aggregation and the Lipid Peroxidation of Platelets Exposed to Hydrogen Peroxide

Higashi, Ototaka; Kikuchi, Yoko

doi:10.1620/tjem.112.271

Cited by 48 publications

(20 citation statements)

References 13 publications

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“…1200 1800 2400 DL-a-tocophkrol (I U /day) FIGURE 6 a-tocopherol content of platelets from five subjects on oral vitamin E supplementation. Each (25). Also, in vitamin E-deficient rats, platelet aggregation induced by collagen was found to be increased compared to that of animals on a normal diet (26).…”

Section: Resultsmentioning

confidence: 92%

Vitamin E. An inhibitor of the platelet release reaction.

Steiner¹,

Anastasi²

1976

J. Clin. Invest.

207

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A B S T R A C T Formation of lipid peroxides rises sharply when platelets undergo the release reaction. In this study the in vitro effect of vitamin E on platelet aggregation was investigated. a-Tocopherol, an antioxidant of known inhibitory action on lipid peroxidation, was added to platelet suspensions in concentrations up to 1.5 mM. A dose-dependent reduction in platelet aggregation was observed, with complete inhibition of the secondary wave of aggregation at > 0.9 mM atocopherol. The inhibitory effect of a-tocopherol on the platelet release reaction was further documented by the decrease in aggregation-induced release of [14C]5-hydroxytryptamine from prelabeled platelets and by the reduction of N-acetylglucosaminidase activity released into the medium. The sharp rise in lipid peroxides normally associated with platelet aggregation was markedly reduced by a-tocopherol and also by acetylsalicylic acid, a known inhibitor of the platelet release reaction.In vivo studies examined the effect of oral vitamin E administration (1,200-2,400 IU daily) on plasma and platelet levels of a-tocopherol. Up to 1,800 IU daily, increasing dosages of vitamin E resulted in increasing concentrations of a-tocopherol in plasma and platelets, but intake of vitamin E in excess of this dosage failed to show any further increase in plasma or platelet levels.

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Section: Resultsmentioning

confidence: 92%

Vitamin E. An inhibitor of the platelet release reaction.

Steiner¹,

Anastasi²

1976

J. Clin. Invest.

207

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show abstract

“…Higashi & Kikuchi (1974) were the first to demonstrate that vitamin E inhibits the aggregation of platelets using hydrogen peroxide as the aggregating stimulus. Subsequent studies by Steiner & Anastasi, (1976) demonstrated that vitamin E also inhibited platelet aggregation response to epinephrine, collagen and ATP.…”

Section: Resultsmentioning

confidence: 99%

Myocardial Ischemia-Reperfusion/Injury

Saleh¹,

Youssef²

2012

Novel Strategies in Ischemic Heart Disease

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“…Mickel and Horbar (1974) reported that the platelet aggregation resulting from exposure to peroxidized arachidonic acid was abolished by prior treatment of the lipid peroxide with tocopherol and butylated hydroxytoluene . Previously we have demonstrated that vitamin E-nicotinate could inhibit lipid peroxide formation of platelets exposed to 11,0, (Higashi and Kikuchi 1974). Therefore the inhibitory effect of vitamin E on the platelet aggregation may be due to the antioxidant activity of vitamin E. But it may also be, at least partly, due to the structural role for vitamin E in the control of functions of membrane (Lucy 1972).…”

Section: Methodsmentioning

confidence: 94%